公开(公告)号：US20230210934A1

公开(公告)日：2023-07-06

申请号：US17652210

申请日：2022-02-23

Applicant: Zhejiang University

Inventor： Shiguo Chen ,  Chengxiao Yu ,  Xinxin Hu ,  Jianle Chen ,  Haibo Pan ,  Xingqian Ye ,  Donghong Liu ,  Tian Ding

IPC: A61K36/815 ,  C08B37/00

CPC classification number: A61K36/815 ,  C08B37/0003

Abstract: Provided are a lycium barbarum leaf polysaccharide rich in galacturonic acid and a preparation method and use thereof. The method includes: mixing a lycium barbarum leaf and an acetone aqueous solution to obtain a mixture, and performing a fading treatment on the mixture to obtain a faded lycium barbarum leaf; extracting the faded lycium barbarum leaf with a chelating agent solution to obtain an extract solution; subjecting the extract solution to an alcohol precipitation with ethanol to obtain an alcohol precipitate; and subjecting the alcohol precipitate to an alcohol washing, a water redissolution, a dialysis and a drying in sequence to obtain the lycium barbarum leaf polysaccharide rich in galacturonic acid.

公开(公告)号：US12275830B2

公开(公告)日：2025-04-15

申请号：US17909285

申请日：2022-03-11

Applicant: ZHEJIANG UNIVERSITY ,  JIANGNAN UNIVERSITY

Inventor： Tian Ding ,  Mofei Shen ,  Donghong Liu ,  Wei Zhao ,  Jinsong Feng

IPC: C08J5/18 ,  C08K3/08 ,  C08K5/13

Abstract: A method for preparing a sustained-release antibacterial film includes: (1) mixing a cyclodextrin metal-organic framework (CD-MOF) material with a silver nitrate short-chain alcohol solution to prepare an intermediate material A; (2) mixing the intermediate material A with a caffeic acid short-chain alcohol solution to prepare an intermediate material B; (3) dispersing the intermediate material B in a solvent, adding a polydimethylsiloxane main agent and a PDMS auxiliary agent in sequence, and mixing well by stirring to obtain a casting solution; and (4) coating the casting solution on a film support material, conducting vacuum drying, and peeling off a film from the film support material to obtain the sustained-release antibacterial film. A mixed matrix-based film material in which the CD-MOF and the PDMS are physically blended is prepared based on CD-MOF and PDMS for the first time, and may be used in application researches of food, environment and other fields.

公开(公告)号：US11952436B2

公开(公告)日：2024-04-09

申请号：US17267487

申请日：2019-12-03

Applicant: ZHEJIANG UNIVERSITY

Inventor： Shiguo Chen ,  Weiwei Hu ,  Xingqian Ye ,  Huan Cheng ,  Donghong Liu ,  Chantapakul Thunthacha

IPC: C08B37/00

CPC classification number: C08B37/0048

Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.

公开(公告)号：US11292852B2

公开(公告)日：2022-04-05

申请号：US16648227

申请日：2017-09-26

Applicant: ZHEJIANG UNIVERSITY

Inventor： Shiguo Chen ,  Hua Zhang ,  Xingqian Ye ,  Donghong Liu ,  Jiaqi Zheng

IPC: C08B37/00

Abstract: The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature. The extracted pectic polysaccharide can be potentially used as functional food ingredients.

公开(公告)号：US12152044B2

公开(公告)日：2024-11-26

申请号：US17617266

申请日：2020-06-11

Applicant: ZHEJIANG UNIVERSITY

Inventor： Tian Ding ,  Dan Wu ,  Mofei Shen ,  Donghong Liu ,  Enbo Xu

IPC: C07F7/00 ,  C01B37/00 ,  C25B1/04 ,  C25B9/19 ,  C25B13/00

Abstract: Disclosed are a zirconium-based metal-organic framework material and a preparation method thereof. Plasma-activated water or strong acid electrolyzed water is added to a raw material system for preparing a zirconium-based metal-organic framework material UiO-66, and they are then subjected to a reaction to obtain a crude product. The crude product is subjected to a post-treatment to obtain the zirconium-based metal-organic framework material UiO-66.

公开(公告)号：US12115515B2

公开(公告)日：2024-10-15

申请号：US17427922

申请日：2019-10-22

Applicant: ZHEJIANG UNIVERSITY

Inventor： Enbo Xu ,  Donghong Liu ,  Jianwei Zhou ,  Huan Cheng ,  Tian Ding ,  Xingqian Ye

IPC: B01J20/24 ,  B01J20/06 ,  B01J20/28 ,  B01J20/30 ,  C02F1/28 ,  C02F101/30

CPC classification number: B01J20/24 ,  B01J20/06 ,  B01J20/2808 ,  B01J20/3085 ,  B01J2220/46 ,  C02F1/286 ,  C02F2101/308

Abstract: A preparation method for a nanopore starch-based adsorbent. The method mainly comprises: constructing a large number of lamellar crystals on surfaces of starch granules, and inducing the formation of a nano-scale pore channel structure by means of the lamellar crystals. A large number of control tests have proved that the flaky crystals have direct influence on the formation of the nano-scale pore channel structure. The construction of a pore channel structure in a starch matrix material greatly increases the specific surface area, and improves the adsorption properties. In addition, the method is beneficial to the introduction of functional particles (magnetic particles), and avoids the problem of pore channel blockage due to first forming pores and then introducing functional particles.

公开(公告)号：US11202797B2

公开(公告)日：2021-12-21

申请号：US16762147

申请日：2018-07-11

Applicant: ZHEJIANG UNIVERSITY

Inventor： Shiguo Chen ,  Lufeng Yan ,  Danli Wang ,  Junhui Li ,  Xingqian Ye ,  Donghong Liu

IPC: A61K31/737 ,  B01D61/14 ,  C08B37/00 ,  A61P7/02 ,  A61P9/10

Abstract: The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc., achieving rapid preparation and high yield.

公开(公告)号：US20240358654A1

公开(公告)日：2024-10-31

申请号：US18641418

申请日：2024-04-21

Applicant: Zhejiang University

Inventor： Enbo Xu ,  Qingqing Zhu ,  Jingsong Feng ,  Haibo Pan ,  Siyu Yao ,  Tian Ding ,  Donghong Liu ,  Xingqian Ye

IPC: A61K9/51 ,  A61K35/00 ,  A61K35/741

CPC classification number: A61K9/5192 ,  A61K9/5161 ,  A61K35/741 ,  A61K2035/115

Abstract: The present disclosure discloses a preparation method of porous starch for encapsulating probiotic, and belongs to the field of food processing. The present disclosure provides porous starch with controllable pore size and morphology by regulating the ratio of amylose to amylopectin (quantitative compounding), keeping starch hydroxyl sites exposed (concentration cultivation), and building interfacial tension to stretch starch chains (convection drying), and uses the porous starch to encapsulate probiotics, which can improve the retention rate of probiotics, reduce the loss of probiotics during food processing and transportation, and thus retain the biological functions of probiotics to a maximum extent.

公开(公告)号：US11985993B2

公开(公告)日：2024-05-21

申请号：US17272654

申请日：2020-05-20

Applicant: ZHEJIANG UNIVERSITY

Inventor： Donghong Liu ,  Enbo Xu ,  Jianwei Zhou ,  Hao Wang ,  Tian Ding ,  Huan Cheng ,  Xingqian Ye

IPC: A23L2/54 ,  A23C3/07 ,  A23C3/08 ,  A23C9/152 ,  A23L2/44 ,  A23L2/50 ,  A61L2/025 ,  B01J19/10 ,  C02F1/36 ,  C25B1/04 ,  C25B9/00

CPC classification number: A23L2/54 ,  A23C3/073 ,  A23C3/085 ,  A23C9/1522 ,  A23L2/44 ,  A23L2/50 ,  A61L2/025 ,  B01J19/10 ,  C02F1/36 ,  C25B1/04 ,  C25B9/00 ,  A23V2002/00

Abstract: The present invention discloses an ultrasound-electrode-nano-porous membrane coupling hydrogen production and sterilization system, which comprises a vessel and a plurality of hydrogen production units arranged in the vessel. The hydrogen production unit comprises a cavity, and a broadband ultrasonic generator and a circular hydrogen production electrode located in the cavity. The circular hydrogen production electrode wraps around the broadband ultrasonic generator. A bottom membrane at the bottom of the cavity is hydrophobic at the inner side and hydrophilic at the outer side, and a side membrane of the cavity is hydrophilic at the inner side and hydrophobic at the outer side and is mounted vertically or obliquely upwards by an angle of 0-45 degrees. The system has the functions of continuously and efficiently facilitating self-circulation micro-flow of a liquid system, hydrogen dissolution (as nano-bubbles), conversion from the interface to the bulk phase and dispersion of bubbles, sterilization and others.

公开(公告)号：US20240027428A1

公开(公告)日：2024-01-25

申请号：US18182377

申请日：2023-03-13

Applicant: ZHEJIANG UNIVERSITY OF TECHNOLOGY

Inventor： Rongfa Guan ,  Mengyu Yang ,  Hao Zhong ,  Xiaofeng Liu ,  Donghong Liu ,  Guozhou Cao ,  Yong Cheng ,  Haitao Shen ,  Jun Huang ,  Kai Yang ,  Yanbin Wang ,  Haizhi Huang

IPC: G01N33/50 ,  C12N5/09

CPC classification number: G01N33/5011 ,  C12N5/0693 ,  C12N2500/36 ,  C12N2500/34 ,  C12N2502/30

Abstract: The present invention discloses a method for building a co-culture model for Caco-2/RAW-264.7 cells induced by lipopolysaccharides and an application of the co-culture model. The method includes: 1) culturing Caco-2 cells and RAW-264.7 cells; 2) inoculating the 5 Caco-2 cells onto an AP side of an upper chamber of a Transwell plate, and incubating; 3) inoculating the RAW-264.7 cells onto a 24-well plate, and incubating; 4) transferring the upper chamber of the Transwell plate into the 24-well plate; 5) dissolving lipopolysaccharides in PBS to prepare a stock solution, filtering, and diluting for later use; and 6) adding an LPS-10% DMEM medium on a BL side, incubating, and forming an intestinal inflammation model.