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公开(公告)号：US20230141130A1
公开(公告)日：2023-05-11
申请号：US17577117
申请日：2022-01-17
Inventor： Zhiqiang Hou , Ziqi Chai , Xipan Shu , Luqin Luan , Shiguo Chen , Xingqian Ye
Abstract: The present disclosure belongs to the technical field of food processing and provides a phenolic stabilized fruit juice and a preparation method thereof. In the present disclosure, plant RG-I pectin polysaccharide is extracted through an ultra-high pressure technology, and then ultra-high pressure technology is used to promote the interaction of pectin polysaccharides and phenols in fruit juice, improve the stability of phenolic substances in fruit juice, and can improve the bioavailability of phenolic substances in fruit juice. The method of the present disclosure improves the added value of products and solves the problem of low bioavailability of phenolic substances in juice.
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公开(公告)号：US11202797B2
公开(公告)日：2021-12-21
申请号：US16762147
申请日：2018-07-11
Applicant: ZHEJIANG UNIVERSITY
Inventor： Shiguo Chen , Lufeng Yan , Danli Wang , Junhui Li , Xingqian Ye , Donghong Liu
IPC: A61K31/737 , B01D61/14 , C08B37/00 , A61P7/02 , A61P9/10
Abstract: The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc., achieving rapid preparation and high yield.
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3.发明公开公开(公告)号：US20230210934A1
公开(公告)日：2023-07-06
申请号：US17652210
申请日：2022-02-23
Applicant: Zhejiang University
Inventor： Shiguo Chen , Chengxiao Yu , Xinxin Hu , Jianle Chen , Haibo Pan , Xingqian Ye , Donghong Liu , Tian Ding
IPC: A61K36/815 , C08B37/00
CPC classification number: A61K36/815 , C08B37/0003
Abstract: Provided are a lycium barbarum leaf polysaccharide rich in galacturonic acid and a preparation method and use thereof. The method includes: mixing a lycium barbarum leaf and an acetone aqueous solution to obtain a mixture, and performing a fading treatment on the mixture to obtain a faded lycium barbarum leaf; extracting the faded lycium barbarum leaf with a chelating agent solution to obtain an extract solution; subjecting the extract solution to an alcohol precipitation with ethanol to obtain an alcohol precipitate; and subjecting the alcohol precipitate to an alcohol washing, a water redissolution, a dialysis and a drying in sequence to obtain the lycium barbarum leaf polysaccharide rich in galacturonic acid.
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公开(公告)号：US11485799B2
公开(公告)日：2022-11-01
申请号：US17267489
申请日：2019-12-03
Applicant: ZHEJIANG UNIVERSITY
Inventor： Xingqian Ye , Weiwei Hu , Shiguo Chen , Huan Cheng , Donghong Liu
IPC: C08B37/00
Abstract: The present disclosure discloses an ultrasonic-assisted method of extracting a pectin rich in RG-I. The method includes: (1) dispersing citrus peel powders in an alkaline solution containing sodium borohydride; (2) placing a solution obtained in step (1) in a water bath of 25 to 40° C., and performing an ultrasonic treatment on the solution obtained in step (1); (3) centrifuging the solution subjected to step (2) at a speed of 6000-8000 rpm to remove residues, retaining a supernatant, and precipitating a pectic polysaccharide with absolute ethanol; and (4) adding water to the pectic polysaccharide precipitant subjected to step (3) to obtain a redissolved solution, transferring the solution to a dialysis bag with a molecular weight cut-off of 3000 to 3500 Da, dialyzing for 48 hours, and freeze-drying in a vacuum freezer to obtain the pectin rich in RG-I.
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公开(公告)号：US20220056159A1
公开(公告)日：2022-02-24
申请号：US17299116
申请日：2020-06-09
Applicant: Zhejiang University
Inventor： Jianle Chen , Xingqian Ye , Huan Cheng , Shiguo Chen , Donghong Liu
IPC: C08B37/00
Abstract: Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin.
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Patent Agency Ranking
公开(公告)号：US11110143B2
公开(公告)日：2021-09-07
申请号：US16754137
申请日：2018-09-27
Applicant: ZHEJIANG UNIVERSITY
Inventor： Shiguo Chen , Jiaqi Zheng , Xingqian Ye , Donghong Liu , Hua Zhang
IPC: A61K36/00 , A61K36/752 , A23L33/00 , A23L33/105 , A23L33/135 , A61K9/08 , A61K9/14
Abstract: The present disclosure discloses a method for preparing a citrus-derived complex prebiotic agent. The method comprises the following steps: removing external citrus peels after blanching citrus fruits with hot water, soaking citrus segments after splitting with an acid solution and an alkali solution to remove membranes of the citrus segments, and adjusting the pH of the obtained alkali soaking water with acid soaking water or acid to be weakly acidic or neutral to obtain the complex prebiotic agent, which has the function of reversing intestinal flora disorders. Compared with the traditional prebiotic product preparing method, the product prepared by the method contains prebiotic functional components with different targets such as pectin, flavonoids, oligosaccharides, and other fibers at the same time, and has the efficacies of regulating intestinal flora and reversing intestinal flora disorders caused by antibiotics. At the same time, the preparing method has the characteristics of simple operation and low cost, perfectly supplements a comprehensive utilization process of citrus fruit processing, solves the discharge problem of citrus fruit processing water, and generates very good social benefits.
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公开(公告)号：US20240302336A1
公开(公告)日：2024-09-12
申请号：US18373398
申请日：2023-09-27
Applicant: ZHEJIANG UNIVERSITY
Inventor： Shiguo Chen , Kai Zhu , Xingqian Ye , Zhiqiang Hou , Jinghua Wu
IPC: G01N30/72
CPC classification number: G01N30/7233 , G01N2030/027 , G01N2030/067
Abstract: The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg/mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.
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公开(公告)号：US11952436B2
公开(公告)日：2024-04-09
申请号：US17267487
申请日：2019-12-03
Applicant: ZHEJIANG UNIVERSITY
Inventor： Shiguo Chen , Weiwei Hu , Xingqian Ye , Huan Cheng , Donghong Liu , Chantapakul Thunthacha
IPC: C08B37/00
CPC classification number: C08B37/0048
Abstract: The present disclosure discloses a method for extracting pectin rich in RG-I, in particular to an ultrasound and pressure assisted method for extracting pectin rich in RG-I. The main process includes: dispersing sieved citrus peel powers in alkaline solution containing sodium borohydride (20 to 50 mM); performing an ultrasound and pressure combined treatment on the above solution at 25 to 40° C., the treatment conditions include: static pressure of 0.2 to 0.4 MPa, ultrasonic intensity of 0.5 to 3.5 W/mL, and treatment time of 20 to 40 min; centrifuging the ultrasonically processed solution to remove residues, and sequentially carrying out precipitation with ethanol (50% to 80% v/v), precipitant dialysis (3000 to 3500 Da), and vacuum freeze drying on the extracted solution to obtain pectic polysaccharide.
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公开(公告)号：US11292852B2
公开(公告)日：2022-04-05
申请号：US16648227
申请日：2017-09-26
Applicant: ZHEJIANG UNIVERSITY
Inventor： Shiguo Chen , Hua Zhang , Xingqian Ye , Donghong Liu , Jiaqi Zheng
IPC: C08B37/00
Abstract: The present disclosure discloses a method for extracting a RG-I-rich pectic polysaccharide. The method comprises the following steps: mixing and stirring a citrus fruit peel powder and a dilute hydrochloric acid solution at a low temperature to destroy cell walls of citrus peels, filtering, and then mixing the retentate with a sodium hydroxide solution for low temperature extraction, adding ethanol to the solution obtained by filtering to precipitate, and washing the obtained polysaccharide with ethanol and drying to obtain the above pectic polysaccharide. Compared with the traditional extraction method, this method reduces the hydrolysis of pectin side chains by controlling the extraction temperature, and the extracted pectic polysaccharide is rich in RG-I domain and has higher biological activity such as cancer and cardiovascular disease prevention. Energy can be greatly saved because of the low extraction temperature. The extracted pectic polysaccharide can be potentially used as functional food ingredients.
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10.发明申请公开(公告)号：US20210106030A1
公开(公告)日：2021-04-15
申请号：US16958668
申请日：2020-01-13
Applicant: ZHEJIANG UNIVERSITY
Inventor： Xingqian Ye , Jianle Chen , Shiguo Chen
Abstract: A process for processing mandarin segments with both acidic and alkaline process water being directly utilized uses an organic acid or an inorganic polyacid instead of a monobasic inorganic acid. Mandarin segments, obtained by separating peeled mandarin into the segments, are treated by the organic acid or inorganic polyacid solution, and then subjected to removal of segment membranes in an alkali solution. The acidic and alkaline water after treatment is collected directly. The collected water can then be filtered and mixed for making an electrolyte beverage or a beverage base, containing multiple functional ingredients such as pectin, flavonoid, pigment, and limonin. Also, the collected water can be used to make dietary fiber having an activity of improving intestinal function.
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