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公开(公告)号：US09587256B2
公开(公告)日：2017-03-07
申请号：US14426290
申请日：2013-09-06
发明人： Michael W. W. Adams , Robert M. Kelly , Aaron B. Hawkins , Angeli Lal Menon , Gina Lynette Pries Lipscomb , Gerrit Jan Schut
IPC分类号： C12N1/20 , C12N1/00 , C12P1/00 , C12P7/40 , C12P7/42 , C07H21/04 , C12N15/52 , C12N9/02 , C12N9/00 , C12N9/88 , C12N9/90
CPC分类号： C12P7/42 , C12N9/0008 , C12N9/001 , C12N9/0036 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12Y102/01018 , C12Y102/01075 , C12Y102/01076 , C12Y103/01084 , C12Y106/99001 , C12Y402/01116 , C12Y501/99001 , C12Y504/99002 , C12Y602/01036 , C12Y604/01002 , C12Y604/01003 , Y02P20/52
摘要： Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions.
摘要翻译： 本文提供了包括至少一部分碳固定通道的遗传工程微生物，并且在一个实施方案中，使用分子氢来驱动二氧化碳固定。 在一个实施方案中，修饰基因工程微生物以将水平大于对照微生物的乙酰辅酶A，分子氢和二氧化碳转化为3-羟基丙酸酯，4-羟基丁酸酯，乙酰辅酶A或其组合。 也可以生产其他产品。 本文还提供了将乙酰辅酶A，分子氢和二氧化碳转化为3-羟基丙酸酯，4-羟基丁酸酯，乙酰辅酶A或其组合的无细胞组合物。 本文还提供了使用遗传工程微生物和无细胞组合物的方法。
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公开(公告)号：US09121045B2
公开(公告)日：2015-09-01
申请号：US14504558
申请日：2014-10-02
申请人： Codexis, Inc.
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
摘要翻译： 本公开涉及具有改进性质的工程化烯酮还原酶多肽，编码工程改造多肽的多核苷酸，相关载体，宿主细胞和用于制备工程化烯酮还原酶多肽的方法。 本公开还提供了使用工程化烯酮还原酶多肽进行化学转化的方法。
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公开(公告)号：US20170175087A1
公开(公告)日：2017-06-22
申请号：US15443148
申请日：2017-02-27
申请人： Codexis, Inc.
IPC分类号： C12N9/02
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
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公开(公告)号：US20160273007A1
公开(公告)日：2016-09-22
申请号：US15176851
申请日：2016-06-08
申请人： Codexis, Inc.
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
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公开(公告)号：US09388438B2
公开(公告)日：2016-07-12
申请号：US14800306
申请日：2015-07-15
申请人： Codexis, Inc.
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
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公开(公告)号：US20150315613A1
公开(公告)日：2015-11-05
申请号：US14800306
申请日：2015-07-15
申请人： Codexis, Inc.
IPC分类号： C12P7/26
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
摘要翻译： 本公开涉及具有改进性质的工程化烯酮还原酶多肽，编码工程改造多肽的多核苷酸，相关载体，宿主细胞和用于制备工程化烯酮还原酶多肽的方法。 本公开还提供了使用工程化烯酮还原酶多肽进行化学转化的方法。
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公开(公告)号：US20130115663A1
公开(公告)日：2013-05-09
申请号：US13658582
申请日：2012-10-23
申请人： Codexis, Inc.
发明人： Christopher Savile , Vesna Mitchell , Xiyun Zhang , Gjalt Huisman
IPC分类号： C12N9/00
CPC分类号： C12N9/001 , C07K2319/00 , C12N9/0036 , C12N15/00 , C12P7/24 , C12P7/26 , C12P7/52 , C12P7/62 , C12P13/002 , C12Y103/01031 , C12Y106/99001 , Y02P20/52
摘要： The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
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