公开(公告)号：US06740517B2

公开(公告)日：2004-05-25

申请号：US10023362

申请日：2001-12-14

申请人： Ming-Huang Lan ,  Li-Yu Wu

发明人： Ming-Huang Lan ,  Li-Yu Wu

IPC分类号： C12N114

CPC分类号： C12N1/14 ,  A01G18/00 ,  A23L31/00 ,  A61K36/07 ,  Y10S435/915

摘要： A solid culture of Zang Zhi (Antrodia camphorata) having triterpenoid compounds, water-soluble polysaccharides and chitin. The solid culture is prepared by a method including the steps of: incubating a stock strain of Zang Zhi in a bag for growth of mycelia, followed by an incubation in the air for fruiting the Zang Zhi body.

摘要翻译： 具有三萜化合物，水溶性多糖和几丁质的ang i（Antrodia camphorata）的固体培养物。 通过包括以下步骤的方法制备固体培养物：将Zang Zhi的原种菌株在菌丝体生长的袋子中孵育，随后在空气中孵育以使枣子体结实。

公开(公告)号：US06709849B2

公开(公告)日：2004-03-23

申请号：US10187113

申请日：2002-06-28

申请人： Ling Yuk Cheung

发明人： Ling Yuk Cheung

IPC分类号： C12N114

CPC分类号： A61K36/062 ,  A23L33/14 ,  A61K2300/00

摘要： Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to treat male hormone deficiency in a subject as a result of having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making such compositions.

摘要翻译： 包含多个酵母细胞的组合物，其中所述多个酵母细胞的特征在于其作为在具有特定频率和特定场的交变电场存在下培养的受试者中的雄激素缺乏的能力 强度。 还包括制备这种组合物的方法。

公开(公告)号：US06645755B1

公开(公告)日：2003-11-11

申请号：US08422612

申请日：1995-04-13

申请人： Stefano Marullo ,  Colette Delavier ,  Laurent Emorine ,  Donny Strosberg

发明人： Stefano Marullo ,  Colette Delavier ,  Laurent Emorine ,  Donny Strosberg

IPC分类号： C12N114

CPC分类号： C07K14/705 ,  C12Q1/6876

摘要： The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eukaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein—called the G protein—able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

摘要翻译： 本发明涉及能够在单细胞生物培养物中复制的载体，该载体含有编码具有膜受体生物学活性的真核蛋白的基因并与调节蛋白相互作用（称为G蛋白 - 能够结合分子 的三磷酸鸟苷（GTP）。 本发明还涉及通过上述载体转化的细胞生物体。 它还涉及用于检测分子表现为受体配体的能力的程序和用于研究受体对配体的亲和力的方法以及用于检测配体对于配体的可能亲和力的试剂盒 受体。

公开(公告)号：US06562614B2

公开(公告)日：2003-05-13

申请号：US09848813

申请日：2001-05-04

申请人： Fang-Jen S. Lee ,  Chun-Fang Huang

发明人： Fang-Jen S. Lee ,  Chun-Fang Huang

IPC分类号： C12N114

CPC分类号： C07K16/40 ,  C07K2317/77 ,  C12N9/14

摘要： The invention relates to a transgenic knockout yeast which has a disruption in the gene encoding for a yeast ADP-ribosylation factor-like protein.

公开(公告)号：US06423530B1

公开(公告)日：2002-07-23

申请号：US09705898

申请日：2000-11-06

申请人： Masaru Tsuboi ,  Shiho Shimizu ,  Michio Yamashita ,  Yasuhisa Tsurumi ,  Seiji Hashimoto

发明人： Masaru Tsuboi ,  Shiho Shimizu ,  Michio Yamashita ,  Yasuhisa Tsurumi ,  Seiji Hashimoto

IPC分类号： C12N114

CPC分类号： C12P17/188 ,  Y10S435/929

摘要： A process for producing a compound of the general formula (I): (wherein R1 represents hydrogen, a lower alkanoyl group or a lower alkyl group; R2 and R3 each represents hydrogen or a lower alkanoyl group) characterized in that a compound of the general formula (II): (wherein R1, R2 and R3 are respectively as defined above) is reacted with a culture of a strain of microorganism belonging to the genus Fusarium or its cells harvested by filtering the broth.

摘要翻译： 一种制备通式（I）的化合物的方法（其中R 1表示氢，低级烷酰基或低级烷基; R 2和R 3各自表示氢或低级烷酰基），其特征在于通式 式（II）:(其中R1，R2和R3分别如上定义）与属于镰刀菌属的微生物菌株或通过过滤肉汤收获的细胞的培养物反应。

公开(公告)号：US06403362B1

公开(公告)日：2002-06-11

申请号：US09230225

申请日：1999-03-03

申请人： Tatsuki Moriya ,  Kouichirou Murashima ,  Kaoru Aoyagi ,  Naomi Sumida ,  Manabu Watanabe ,  Toru Hamaya ,  Jinichiro Koga ,  Toshiaki Kono ,  Takeshi Murakami

发明人： Tatsuki Moriya ,  Kouichirou Murashima ,  Kaoru Aoyagi ,  Naomi Sumida ,  Manabu Watanabe ,  Toru Hamaya ,  Jinichiro Koga ,  Toshiaki Kono ,  Takeshi Murakami

IPC分类号： C12N114

CPC分类号： C12N9/2437 ,  C12N15/80 ,  C12Y302/01004 ,  Y10S530/82 ,  Y10S530/823

摘要： An expressing system which enables a large amount of production of a protein in Humicola, in particular, Humicola insolens, and particularly host-vector systems and a process for producing a protein using the systems, wherein an expression vector comprising the regulator sequences, i.e., the promoter, the signal sequence, and the terminator, of the cellulase NCE1 gene or NCE2 gene derived from Humicola insolens is constructed and used. The expression vector enables highly efficient production of cellulase NCE4, for example, in Humicola insolens at a rate as high as about 4.5 g or more per one liter of culture.

摘要翻译： 一种表达系统，其能够在Humicola中大量生产蛋白质，特别是Humicola insolens，特别是宿主 - 载体系统，以及使用该系统产生蛋白质的方法，其中包含调节序列的表达载体， 构建并使用来自Humicola insolens的纤维素酶NCE1基因或NCE2基因的启动子，信号序列和终止子。 表达载体能够高效率地生产纤维素酶NCE4，例如在Humicola insolens中以每升培养物高达约4.5g或更高的速率。

公开(公告)号：US06391617B1

公开(公告)日：2002-05-21

申请号：US09797371

申请日：2001-03-01

申请人： Ling Yuk Cheung

发明人： Ling Yuk Cheung

IPC分类号： C12N114

CPC分类号： C12N13/00 ,  C12N1/16

摘要： Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to convert bio-available nitrogen in a culture medium into their own biomass. Also included are methods of making such compositions.

摘要翻译： 包含多个酵母细胞的组合物，其中所述多个酵母细胞已经在具有特定频率和比电场强度的交变电场的存在下培养足够长的时间，以足以显着增加所述多种酵母的能力 将培养基中的生物可利用氮转化成其自身的生物质。 还包括制备这种组合物的方法。

公开(公告)号：US06383799B1

公开(公告)日：2002-05-07

申请号：US09419207

申请日：1999-10-15

申请人： Solomon P. Wasser ,  Sergey V. Reshetnikov

发明人： Solomon P. Wasser ,  Sergey V. Reshetnikov

IPC分类号： C12N114

CPC分类号： C12N1/14 ,  A61K36/07 ,  C12P19/04

摘要： The present invention describes new and distinct strains of higher Basidiomycetes mushrooms grown in submerged cultures. Specifically, the new strain of species of the genus Tremella offer superior yields of one-cell biomass and exocellular heteropolysaccharide glucuronoxylomannan, niacin and essential amino acids.

公开(公告)号：US06355473B1

公开(公告)日：2002-03-12

申请号：US09305923

申请日：1999-05-05

申请人： Kirill Ostanin ,  Lauren Silverman

发明人： Kirill Ostanin ,  Lauren Silverman

IPC分类号： C12N114

CPC分类号： G01N33/566 ,  C07K14/39 ,  C07K14/723 ,  G01N2500/10

摘要： The invention provides yeast cells which comprise a mutation in an endogenous yeast stp22 gene and which express a heterologous G protein coupled receptor. The instant yeast cells display enhanced sensitivity to ligand induced stimulation of heterologous G protein coupled receptors and, therefore, show improved properties in drug screening assays.

摘要翻译： 本发明提供了酵母细胞，其包含内源酵母stp22基因中的突变并表达异源G蛋白偶联受体。 即时酵母细胞对配体诱导的异源G蛋白偶联受体的刺激显示增强的灵敏度，因此在药物筛选测定中显示出改进的性质。

公开(公告)号：US06204049B1

公开(公告)日：2001-03-20

申请号：US08599260

申请日：1996-02-09

申请人： Joan Wennstrom Bennett ,  Adele Marie Childress ,  Kenneth George Wunch ,  William Joseph Connick, Jr.

发明人： Joan Wennstrom Bennett ,  Adele Marie Childress ,  Kenneth George Wunch ,  William Joseph Connick, Jr.

IPC分类号： C12N114

CPC分类号： C12N1/14

摘要： Compositions and methods for bioremediation of sites contaminated with chemical pollutants, wherein survival and colonization of a polluted medium by a fungal microorganism having the capacity to degrade a chemical pollutant is enhanced; and microorganisms having the capacity to degrade one or more priority pollutants.

摘要翻译： 对化学污染物污染地点进行生物修复的组合物和方法，其中具有降解化学污染物质的真菌微生物的污染培养基的存活和定植得到增强; 以及具有降解一种或多种优先污染物的能力的微生物。