公开(公告)号：US06746862B1

公开(公告)日：2004-06-08

申请号：US09611992

申请日：2000-07-07

申请人： Wen-Teng Wu ,  Pei-Ming Wang ,  Ting-Kuo Huang ,  Gwo-Fang Yuan

发明人： Wen-Teng Wu ,  Pei-Ming Wang ,  Ting-Kuo Huang ,  Gwo-Fang Yuan

IPC分类号： C12N114

CPC分类号： C12N1/14

摘要： The invention discloses a method for cultivation of filamentous fungi using a suspended nutritionally solid substrate, the method including the steps of: (a) preparing a medium comprising a nutritionally solid substrate; and (b) inoculating an inoculum into the medium comprising the nutritionally solid substrate in a bioreactor to carry out fermentation. The present invention combines the properties of solid-state culture and submerged culture. In addition, the present invention may employ an air-lift bioreactor with a net draft tube and the fed-batch process to elevate the yields of filamentous fungi and metabolites thereof.

摘要翻译： 本发明公开了一种使用悬浮营养固体基质培养丝状真菌的方法，所述方法包括以下步骤：（a）制备包含营养固体底物的培养基; 和（b）在生物反应器中将接种物接种到包含营养固体底物的培养基中以进行发酵。 本发明结合了固相培养和浸没培养的性质。 此外，本发明可以采用具有净引流管的空气提升生物反应器和补料分批方法来提高丝状真菌及其代谢物的产率。

公开(公告)号：US06503749B2

公开(公告)日：2003-01-07

申请号：US09797239

申请日：2001-03-01

申请人： Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse

发明人： Tilman Achstetter ,  Gilles Cauet ,  Eric Degryse

IPC分类号： C12N114

CPC分类号： C12N9/1029 ,  C12P33/00

摘要： A subject of the invention is a modified yeast strain in which the acetyl-CoA pregnenolone acetyltransferase (APAT) activity is eliminated by altering the gene coding for this activity with resultant stabilization of 3&bgr;-hydroxysteroids.

摘要翻译： 本发明的主题是修饰的酵母菌株，其中通过改变编码该活性的基因来消除乙酰辅酶A孕烯醇酮乙酰转移酶（APAT）活性，导致3β-羟基类固醇的稳定。

公开(公告)号：US06436695B1

公开(公告)日：2002-08-20

申请号：US09797381

申请日：2001-03-01

申请人： Ling Yuk Cheung

发明人： Ling Yuk Cheung

IPC分类号： C12N114

CPC分类号： B09C1/10 ,  C12N1/16 ,  C12N13/00

摘要： Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to convert biologically available phosphorus in a culture medium into their own biomass. Also included are methods of making such compositions.

摘要翻译： 包含多个酵母细胞的组合物，其中所述多个酵母细胞已经在具有特定频率和比电场强度的交变电场的存在下培养足够长的时间，以足以显着增加所述多种酵母的能力 将培养基中的生物利用磷转化成其自身的生物质的细胞。 还包括制备这种组合物的方法。

公开(公告)号：US06391618B1

公开(公告)日：2002-05-21

申请号：US09797378

申请日：2001-03-01

申请人： Ling Yuk Cheung

发明人： Ling Yuk Cheung

IPC分类号： C12N114

CPC分类号： C12N13/00 ,  C12N1/16 ,  C12P1/02

摘要： Compositions comprising a plurality of yeast cells which have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to degrade environmental toxins, such as antibiotics and organic solvents, in a culture medium. Also included are methods of making such compositions.

摘要翻译： 包含多个酵母细胞的组合物，其已经在具有特定频率和特定场强的交变电场的存在下培养足以显着增加所述多个酵母细胞降解环境毒素的能力的时间段， 如抗生素和有机溶剂，在培养基中。 还包括制备这种组合物的方法。

公开(公告)号：US06387688B1

公开(公告)日：2002-05-14

申请号：US09503505

申请日：2000-02-14

申请人： Kazuo Shishido ,  Susumu Kajiwara ,  Akira Tsukamoto

发明人： Kazuo Shishido ,  Susumu Kajiwara ,  Akira Tsukamoto

IPC分类号： C12N114

CPC分类号： C12N9/0061 ,  C07K14/37 ,  C12N9/0065

摘要： This invention relates to a Coriolus hirsutus host cell transformed with a vector containing a basidiomycete-derived promoter region selected from the group consisting of basidiomycete-derived ras and priA gene promoter regions. This invention is particularly characterized in that the basidiomycete-derived promoter region is a Lentinus edodes-derived ras or priA gene promoter region or a Coriolus hirsutus-derived ras gene promoter region. This host cell can be utilized for the high productivity of lignin degrading enzymes.

摘要翻译： 本发明涉及一种用含有担子菌纲衍生的启动子区域的载体转化的陆地毛球菌宿主细胞，该区域选自担纲菌来源的ras和priA基因启动子区域。 本发明特别的特征在于担纲菌来源的启动子区域是香菇来源的ras或priA基因启动子区域或来自陆地弯曲菌的ras基因启动子区域。 该宿主细胞可用于木质素降解酶的高生产率。

公开(公告)号：US06306390B1

公开(公告)日：2001-10-23

申请号：US09194289

申请日：1999-04-12

申请人： Kazuhiko Narisawa

发明人： Kazuhiko Narisawa

IPC分类号： C12N114

CPC分类号： A01N63/04

摘要： Root endophyte which live symbiotically within a root of crops were separated and classified. A medium which cultured the strains were inoculated with a seed of a crop. The seed was allowed to sprout to inoculate the root of the crop with root endophytes. While growing the crop, the soil for growth was inoculated with soil pathogenic bacteria, and the crop was grown for a period necessary for the occurrence of a soil disease. The crop was inspected for pathopoiesis, and root endophytes, which had been inoculated into the root of crop having low severity, were specified and selected. As a result, it was found that some root endophytes can significantly inhibit soil diseases. Among root endophytes, Heteroconium chaetospira was found to be particularly effective in inhibiting soil diseases.

摘要翻译： 分离和分类在作物根部共生的根内生真菌。 培养菌株的培养基用作物种子接种。 允许种子发芽以用根内生菌接种作物的根部。 在种植作物的同时，用土壤病原菌接种生长土壤，作物生长一段土壤病发生所需的时间。 对作物进行了病虫害检查，并指定并选择了已经接种到具有低严重程度的作物根中的根内生真菌。 结果发现一些根内生真菌可以显着抑制土壤病害。 在根内生真菌中，发现Heteroconium chaetospira在抑制土壤病害方面特别有效。

公开(公告)号：US06284521B1

公开(公告)日：2001-09-04

申请号：US09601509

申请日：2000-09-20

申请人： Roman Vladimirovich Borovick ,  Gennady Alekseevich Zharikov ,  Vladimir Vladimirovich Kapranov

发明人： Roman Vladimirovich Borovick ,  Gennady Alekseevich Zharikov ,  Vladimir Vladimirovich Kapranov

IPC分类号： C12N114

CPC分类号： C12R1/78 ,  B09C1/10 ,  C02F3/34 ,  C02F2101/363

摘要： Hansenulla californica yeast strain VKPM Y-2284 is capable of degrading polychlorinated biphenyls (PCBs). The strain may be employed to detoxicate environment media and PCB-containing industrial wastes. To produce biomass, the strain is incubated on media which contain carbon sources, nitrogen sources and mineral salts. The strain is cultivated by a subsurface method up to a titer from 5.0·106 to 1.0×107 cells per cu cm. The produced biomass is employed for degrading PCBs in concentrations from 106 to 105 cells per cu cm. The strain ensures from 30 to 50% reduction in PCB content in soil and water.

摘要翻译： 汉逊酵母菌株VKPM Y-2284能够降解多氯联苯（PCBs）。 该菌株可用于对环境介质和含PCB工业废物进行排毒。 为了生产生物质，将菌株在含有碳源，氮源和矿物盐的培养基上孵育。 通过地下方法培养菌株，直至每立方厘米5.0.106至1.0×10 7个细胞的滴度。 生产的生物质用于降解PCBs，浓度为每立方厘米106至105个细胞。 该应变确保土壤和水中PCB含量降低30％至50％。

公开(公告)号：US06274370B1

公开(公告)日：2001-08-14

申请号：US08895383

申请日：1997-07-16

申请人： John Charles Hobson ,  Roderick Norman Greenshields

发明人： John Charles Hobson ,  Roderick Norman Greenshields

IPC分类号： C12N114

CPC分类号： A61K8/99 ,  A23L5/42 ,  A23L33/14 ,  A61K9/5068 ,  A61Q1/02 ,  C09B61/00 ,  C09B67/0061 ,  C12N1/16 ,  Y10S435/82 ,  Y10S435/822

摘要： The present invention provides a coloring agent comprising glucan-containing yeast cell ghosts which comprise a proportion of substantially intact yeast cell walls and at least one color source. The color source is selected to give the desired color in the coloring agent and in any product in which the coloring agent is used and may be any dye or pigment. For uses such as in foodstuffs, pharmaceuticals and cosmetics, the coloring agents must be acceptable for food and/or pharmaceutical use.

摘要翻译： 本发明提供了含有含葡聚糖的酵母细胞幽灵的着色剂，其包含一定比例的基本上完整的酵母细胞壁和至少一种颜色源。 选择颜色源以在着色剂和使用着色剂的任何产品中产生所需的颜色，并且可以是任何染料或颜料。 对于诸如食品，药品和化妆品的用途，着色剂必须是食品和/或药物用途的可接受的。

公开(公告)号：US06255100B1

公开(公告)日：2001-07-03

申请号：US09392282

申请日：1999-09-09

申请人： Soo Young Ko ,  Hans Kobel ,  Brigitte Besemer-Rosenwirth ,  Dieter Seebach ,  RenéP. Traber ,  Roland Wenger ,  Pietro Bollinger

发明人： Soo Young Ko ,  Hans Kobel ,  Brigitte Besemer-Rosenwirth ,  Dieter Seebach ,  RenéP. Traber ,  Roland Wenger ,  Pietro Bollinger

IPC分类号： C12N114

CPC分类号： A61K38/13 ,  C07K7/645 ,  Y10S435/911 ,  Y10S930/27

摘要： It has been found that nonimmunosuppressive, cyclophilin-binding cyclosporins are useful in the treatment and prevention of AIDS and AIDS-related disorders. Such cyclosporins include novel Ciclosporin derivatives modified at the 4- and/or 5-positions.

摘要翻译： 已经发现，非免疫抑制性亲环蛋白结合环孢菌素可用于治疗和预防艾滋病和AIDS相关疾病。 这种环孢菌素包括在4-和/或5-位修饰的新的环孢菌素衍生物。

公开(公告)号：US06214605B1

公开(公告)日：2001-04-10

申请号：US09555431

申请日：2000-05-30

申请人： Saburo Chida

发明人： Saburo Chida

IPC分类号： C12N114

CPC分类号： C12R1/645 ,  C12P7/18

摘要： Trichosporonoides kounosuensis 7E-1 (FERM BP-6572) having taxonomical properties different from those of any known species belonging to the genus Trichosporonoides, and having the ability to efficiently produce a polyol such as erythritol.

摘要翻译： 具有与属于Trichosporonoides属的任何已知物种的分类学性质的Trichosporonoides kounosuensis 7E-1（FERM BP-6572），并且具有有效生产多元醇如赤藓糖醇的能力。