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    KETOREDUCTASE MUTANT
    1.
    发明申请
    KETOREDUCTASE MUTANT 有权
    KETOREDUCTASE突变体

    公开(公告)号：US20130116416A1

    公开(公告)日：2013-05-09

    申请号：US13699206

    申请日：2010-05-21

    申请人： Fusuke Mazuka ,  Takayoshi Fukushima ,  Naomi Sumida ,  Koji Yanai

    发明人： Fusuke Mazuka ,  Takayoshi Fukushima ,  Naomi Sumida ,  Koji Yanai

    IPC分类号： C12N9/02 ,  C12N1/20

    CPC分类号： C12P19/56 ,  C12N9/0006 ,  C12Y101/01184

    摘要： Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.

    摘要翻译： 公开了可用于有效生产柔红霉素衍生物的酮还原酶突变体，编码突变体的DNA，通过将DNA引入其中以产生柔红霉素衍生物而制备的转化体，以及使用转化体产生柔红霉素衍生物的方法。 酮还原酶突变体具有氨基酸序列，其中选自氨基酸中的一个氨基酸残基或两个或更多个氨基酸残基，所述氨基酸位于与氨基酸中第42,149,153,270和306位氨基酸相对应的位置的氨基酸 来自产生氯霉素的细菌（Amycolatopsis orientalis）的酮还原酶（EvaE）的酸序列被另一个氨基酸残基取代。

    Transformant and method for production of non-natural antibiotic
    2.
    发明授权
    Transformant and method for production of non-natural antibiotic 有权
    用于生产非天然抗生素的转化体和方法

    公开(公告)号：US08383392B2

    公开(公告)日：2013-02-26

    申请号：US12678101

    申请日：2008-09-12

    申请人： Naomi Sumida ,  Manabu Watanabe ,  Koji Yanai

    发明人： Naomi Sumida ,  Manabu Watanabe ,  Koji Yanai

    IPC分类号： C12N1/15 ,  C12P29/00

    CPC分类号： C12P19/56 ,  C12N9/0006 ,  C12P17/06 ,  C12P19/44

    摘要： Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.

    摘要翻译： 公开了通过将参与L-赖氨替康的生物合成的酮还原酶基因导入本来能够产生柔红霉素的放线杆菌中制备的转化体。 还公开了使用转化体有效生产非天然柔红霉素衍生物的方法。 转化体能够有效地产生非天然柔红霉素衍生物如表柔比星。

    METHOD FOR PRODUCTION OF NON-NATURAL ANTIBIOTIC
    3.
    发明申请
    METHOD FOR PRODUCTION OF NON-NATURAL ANTIBIOTIC 有权
    生产非天然抗生素的方法

    公开(公告)号：US20100255543A1

    公开(公告)日：2010-10-07

    申请号：US12678101

    申请日：2008-09-12

    申请人： Naomi Sumida ,  Manabu Watanabe ,  Koji Yanai

    发明人： Naomi Sumida ,  Manabu Watanabe ,  Koji Yanai

    IPC分类号： C12P19/56 ,  C12N1/21

    CPC分类号： C12P19/56 ,  C12N9/0006 ,  C12P17/06 ,  C12P19/44

    摘要： Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.

    摘要翻译： 公开了通过将参与L-赖氨替康的生物合成的酮还原酶基因导入本来能够产生柔红霉素的放线杆菌中制备的转化体。 还公开了使用转化体有效生产非天然柔红霉素衍生物的方法。 转化体能够有效地产生非天然柔红霉素衍生物如表柔比星。

    Saponin-digesting enzymes, genes thereof and soyasapogenol B mass production system
    6.
    发明授权
    Saponin-digesting enzymes, genes thereof and soyasapogenol B mass production system 失效
    皂苷消化酶，其基因和大豆皂甙B大量生产系统

    公开(公告)号：US07022508B2

    公开(公告)日：2006-04-04

    申请号：US10479787

    申请日：2002-06-06

    申请人： Manabu Watanabe ,  Naoki Mido ,  Takayoshi Tamura ,  Naomi Sumida ,  Takashi Yaguchi

    发明人： Manabu Watanabe ,  Naoki Mido ,  Takayoshi Tamura ,  Naomi Sumida ,  Takashi Yaguchi

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/24 ,  C12P33/00 ,  C12R1/885

    CPC分类号： C12N9/2402 ,  C12N15/8243 ,  C12P33/00

    摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

    摘要翻译： 本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质 使用相同 根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质 ，4和6; （b）与包含（a）中描述的序列的氨基酸序列的蛋白质具有至少50％同源性且具有皂苷分解活性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂角苷分解活性的修饰的氨基酸序列的蛋白质。