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    CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING
    11.
    发明申请
    CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING 审中-公开
    通过遗传工程构建新型番茄红素DSR-S的新品种

    公开(公告)号：US20150344853A1

    公开(公告)日：2015-12-03

    申请号：US14798518

    申请日：2015-07-14

    申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE

    发明人： Pierre F. MONSAN ,  Magali REMAUD-SIMEON ,  Gabrielle POTOCKI-VERONESE ,  Claire MOULIS

    IPC分类号： C12N9/10 ,  C07H3/06 ,  C08B37/02 ,  C12P19/08 ,  C12P19/12

    CPC分类号： C12N9/1051 ,  C07H3/06 ,  C07K2319/35 ,  C08B37/0021 ,  C12P19/04 ,  C12P19/08 ,  C12P19/12 ,  C12P19/18 ,  C12Y204/01005 ,  Y02P20/52

    摘要： The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.

    摘要翻译： 本发明涉及一种在保护酶活性或其合成α-1,6键的特异性的同时生产截短或突变的葡聚糖蔗糖酶的重组方法。 本发明涉及截短或突变的葡聚糖蔗糖酶的核酸序列，含有核酸序列的载体和由编码截短或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。 另一方面，本发明涉及一种以重组方式生产截短或突变的葡聚糖蔗糖酯的方法，其保留其酶活性或在合成α-1,6键时保护其特异性，并可从蔗糖，葡萄糖， 与用天然酶和具有受控摩尔质量的异麦芽低聚糖和葡聚糖获得的葡聚糖的性质相比，具有高的摩尔质量和改进的流变学性质。 本发明的葡聚糖和异麦芽低聚糖可以用作变形剂或益生元。

    Food product containing novel dried compositions with polysaccharides
    12.
    发明授权
    Food product containing novel dried compositions with polysaccharides 失效
    含有多糖的新型干燥组合物的食品

    公开(公告)号：US4877634A

    公开(公告)日：1989-10-31

    申请号：US238415

    申请日：1988-08-31

    申请人： Michael J. Pucci ,  Blair S. Kunka

    发明人： Michael J. Pucci ,  Blair S. Kunka

    IPC分类号： A23L29/269 ,  C12P19/08

    CPC分类号： C12P19/08 ,  A23L29/269 ,  A23L29/273

    摘要： Novel dried compositions containing polysaccharides (dextrans and levans) derived using a Leuconostoc to ferment sucrose to produce the polysaccharides are described. In particular, dried compositions incorporating milk solids or other drying aids to facilitate drying and rehydration of the polysaccharides are described. The compositions are useful as quality (e.g. texture, stability or thickness) improvers for foods.

    摘要翻译： 描述了含有使用明串珠糖衍生物发酵蔗糖以产生多糖的多糖（葡聚糖和左旋糖酐）的新型干燥组合物。 特别地，描述了包含乳固体或其它干燥助剂的干燥组合物以促进多糖的干燥和再水化。 该组合物可用作食品的质量（例如质地，稳定性或厚度）改进剂。

    Process for the manufacture of iso-malto-oligosaccharide monovalent haptens
    13.
    发明授权
    Process for the manufacture of iso-malto-oligosaccharide monovalent haptens 失效
    制备异麦芽寡糖单价半抗原的方法

    公开(公告)号：US4673643A

    公开(公告)日：1987-06-16

    申请号：US743715

    申请日：1985-06-11

    申请人： Dieter Schwengers

    发明人： Dieter Schwengers

    IPC分类号： C12P19/08 ,  C12P19/18

    CPC分类号： C12P19/18 ,  C12P19/08

    摘要： Iso-malto-oligosaccharide monovalent haptens were prepared by adding an aqueous sucrose solution to an aqueous solution of D-glucose containing more than 300 mmol glucose per 1000 U .alpha.(1.fwdarw.6)-D-glucosyl transferase at 265 to 310 K and a pH value of from 4.5 to 8.0 and a molar ratio of sucrose to glucose of from 0.5 to 2.0. After consumption of the sucrose, glucose, liberated fructose and undesired oligosaccharides are separated in a known manner.The process of the invention allows a particularly economical preparation of the monovalent haptens which serve for the prophylaxis of undesired dextran induced anaphylactoid side effects (DIAR).

    摘要翻译： 通过在265至310K下，每1000Uα（1-> 6）-D-葡糖基转移酶含有超过300mmol葡萄糖的D-葡萄糖水溶液中加入蔗糖水溶液制备异麦芽低聚糖单价半抗原， pH值为4.5〜8.0，蔗糖与葡萄糖的摩尔比为0.5〜2.0。 消耗蔗糖后，以已知的方式分离葡萄糖，释放的果糖和不想要的低聚糖。 本发明的方法允许特别经济地制备用于预防不期望的葡聚糖诱导的过敏性副作用（DIAR）的单价半抗原。

    Gluco-oligosaccharide mixture and a process for its manufacture
    14.
    发明授权
    Gluco-oligosaccharide mixture and a process for its manufacture 失效
    葡糖低聚糖混合物及其制备方法

    公开(公告)号：US4649058A

    公开(公告)日：1987-03-10

    申请号：US741394

    申请日：1985-06-05

    申请人： Dieter Schwengers

    发明人： Dieter Schwengers

    IPC分类号： A23L27/30 ,  C12P19/08 ,  C12P19/18 ,  C12D13/02 ,  A23L1/236

    CPC分类号： C12Y204/01005 ,  A23L27/33 ,  C12P19/08 ,  C12P19/18 ,  Y10S426/804

    摘要： A gluco-oligosaccharide mixture having up to 30, in particular from 10 to 20, anhydroglucose units is prepared by reacting an aqueous solution of a mono-or disaccharide composed of glucose units containing more than 200 mmol of the saccharide per 1000 U .alpha.(1.fwdarw.6)-D-glucosyl transferase, at 265 to 310 K and a pH value of from 4.5 to 8.0, with an aqueous solution of sucrose in a molar ratio of sucrose to glucose of 2.0 to 5.0.The oligosaccharide mixtures of the present invention are used as calorie-free carrier for sweetening agents and as "body building" sweetening agent.

    摘要翻译： 通过使含有超过200mmol糖的单糖或二糖的单糖或二糖的水溶液每1000Uα（1）含有多达30个，特别是10至20个的葡萄糖 - 寡糖混合物， - 6）-D-葡糖基转移酶，265至310K，pH值为4.5至8.0，蔗糖与葡萄糖的摩尔比为2.0至5.0的蔗糖水溶液。 本发明的寡糖混合物用作甜味剂的无卡路里载体和“健美”甜味剂。