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26 条记录 (耗时 0.021 秒)

    Polyclonal-monoclonal ELISA assay for detecting N-terminus pro-BNP
    21.
    发明授权
    Polyclonal-monoclonal ELISA assay for detecting N-terminus pro-BNP 有权

    公开(公告)号：US11016106B2

    公开(公告)日：2021-05-25

    申请号：US15181283

    申请日：2016-06-13

    申请人： Nexus DX, Inc.

    发明人： Michelle Davey ,  George Jackowski ,  Peter Kupchak ,  Eric Stanton

    IPC分类号： G01N33/74 ,  C07K16/26 ,  G01N33/535 ,  C12N5/16 ,  G01N33/543 ,  G01N33/68 ,  G01N33/545

    摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

    POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PRO-BNP
    24.
    发明申请
    POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PRO-BNP 有权
    用于检测N-末端PRO-BNP的多环 - 单克隆ELISA测定

    公开(公告)号：US20120107848A1

    公开(公告)日：2012-05-03

    申请号：US13236447

    申请日：2011-09-19

    申请人： Michelle DAVEY ,  George JACKOWSKI ,  Peter KUPCHAK ,  Eric STANTON

    发明人： Michelle DAVEY ,  George JACKOWSKI ,  Peter KUPCHAK ,  Eric STANTON

    IPC分类号： G01N33/566

    CPC分类号： G01N33/74 ,  C07K16/26 ,  C07K2317/31 ,  C07K2317/34 ,  C12N5/163 ,  G01N33/535 ,  G01N33/543 ,  G01N33/545 ,  G01N33/6893 ,  G01N2333/58 ,  G01N2800/325 ,  G01N2800/56 ,  G01N2800/7019 ,  Y10S436/811 ,  Y10S530/80

    摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

    摘要翻译： 公开了一种特异性和灵敏的体外ELISA测定和诊断测试试剂盒，用于测定各种体液中NT-proBNP蛋白的水平，其非限制性实例是血液，血清，血浆，尿液等。 NT-proBNP ELISA测定采用夹心ELISA技术测定人血浆中的NT-proBNP循环。 为获得具有针对人proBNP靶向氨基酸序列的特异性结合特性的抗体，重组人proBNP（或rhproBNP）被表达和纯化，用作免疫原。 随后通过连续亲和纯化从山羊血清中纯化特异性氨基酸序列的多克隆抗体（PAb）。 针对特定多肽产生单克隆抗体。 表达和纯化重组人NT-proBNP（或rhNT-proBNP），以获得用于校准人NT-proBNP的定量方法的材料。

    Method for diagnosing and distinguishing stroke and diagnostic devices for use therein
    25.
    发明授权
    Method for diagnosing and distinguishing stroke and diagnostic devices for use therein 有权
    用于诊断和区分用于其中的卒中和诊断装置的方法

    公开(公告)号：US07955811B2

    公开(公告)日：2011-06-07

    申请号：US12698032

    申请日：2010-02-01

    申请人： George Jackowski

    发明人： George Jackowski

    IPC分类号： G01N31/00 ,  G01N33/53

    CPC分类号： G01N33/6896 ,  G01N2800/2871

    摘要： A method for determining whether a subject has had a stroke and, if so, the type of stroke which includes analyzing the subject's body fluid for at least four selected markers of stroke, namely, myelin basic protein, S100 protein, neuronal specific enolase and a brain endothelial membrane protein such as thrombomodulin or a similar molecule. The data obtained from the analyses provide information as to the type of stroke, the onset of occurrence and the extent of brain damage and allow a physician to determine quickly the type of treatment required by the subject.

    摘要翻译： 一种用于确定受试者是否具有中风的方法，如果是，则包括分析至少四种选择的中风标志物的主体体液的中风类型，即髓磷脂碱性蛋白，S100蛋白，神经元特异性烯醇化酶和 脑内皮细胞膜蛋白如血栓调节蛋白或类似分子。 从分析获得的数据提供关于中风类型，发生发生和脑损伤程度的信息，并允许医生快速确定受试者所需的治疗类型。