公开(公告)号：US20220128544A1

公开(公告)日：2022-04-28

申请号：US17430457

申请日：2020-02-13

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel L. ADAMS ,  Cha-Mei TANG

IPC: G01N33/50 ,  G01N33/574

Abstract: Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number of CCR5 pools in circulating cells, such as circulating cancer associated macrophage-like cells (CAMLs) and circulating tumor cells (CTCs) found in a biological sample, such as blood, from the subject. CCR5 expression can also be used for companion or complementary diagnostics.

公开(公告)号：US20220034888A1

公开(公告)日：2022-02-03

申请号：US17503872

申请日：2021-10-18

Applicant: CREATV MICROTECH INC.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

公开(公告)号：US11175279B2

公开(公告)日：2021-11-16

申请号：US16454937

申请日：2019-06-27

Applicant: Creatv MicroTech, Inc.

Inventor： Olga Makarova ,  Cha-Mei Tang ,  Peixuan Zhu ,  Shuhong Li ,  Daniel Adams ,  Platte T. Amstutz

IPC: G01N33/50 ,  B01D71/46 ,  G01N1/40 ,  B01L3/00 ,  B01D39/16 ,  B01D29/00 ,  B01L9/00

Abstract: A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells.

公开(公告)号：US11156596B2

公开(公告)日：2021-10-26

申请号：US16369730

申请日：2019-03-29

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel Adams ,  Cha-Mei Tang

IPC: G01N33/49 ,  G01N33/574 ,  G01N33/487 ,  G01N33/569

Abstract: A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US20210041445A1

公开(公告)日：2021-02-11

申请号：US16979742

申请日：2019-03-13

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574

Abstract: Means for monitoring treatment response and disease progression in subjects are disclosed, where the predictions are based on the change of number and/or size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological ample, such as blood, from the subject.

公开(公告)号：US20200049713A1

公开(公告)日：2020-02-13

申请号：US16498581

申请日：2018-04-02

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: The characterization of nucleic acids obtained from cancer-associated cells circulating in the blood of a subject, and the use of such characterizations in cancer screening, diagnostics, treatment, and recurrence, are disclosed.

公开(公告)号：US20190262778A1

公开(公告)日：2019-08-29

申请号：US16265305

申请日：2019-02-01

Applicant: Creatv MicroTech, Inc.

Inventor： Cha-Mei Tang ,  Olga Makarova

IPC: B01D67/00 ,  A61M1/34 ,  B01D69/12 ,  C12M1/00 ,  B01D71/46 ,  B01D71/64

Abstract: Micro- and nanofilters with precision pore sizes and pore layout have applications in many fields including capturing circulating tumor cells and fetal cells in blood, water treatment, pathogen detection in water, etc. Methods to fabricate micro- and nanofilters not using track etching or reactive ion etching are provided, allowing easy fabrication of single layer or stack of films simultaneously, and/or stack of films on rolls. Microfilter can be made using one or more layers of material. Invention enables mass production of microfilters with lithographic quality at low cost. Isolation, enumeration and characterization of circulating tumor cells using microfilters provides (i) guides to cancer treatment selection and personalize dosage, (ii) low cost monitoring for treatment response, disease progression and recurrence, (iii) assessment of pharmacodynamic effects, (iv) information on mechanisms of resistance to therapy, and (v) cancer staging. Microfabrication methods are also applicable to fabrication of any free standing patterned polymeric films.

公开(公告)号：US20190242871A1

公开(公告)日：2019-08-08

申请号：US16369730

申请日：2019-03-29

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/49 ,  G01N33/574 ,  G01N33/487

CPC classification number: G01N33/491 ,  G01N33/48735 ,  G01N33/57415 ,  G01N33/57419 ,  G01N33/57423 ,  G01N33/57434

Abstract: A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

公开(公告)号：US20190128892A1

公开(公告)日：2019-05-02

申请号：US16092998

申请日：2017-04-14

Applicant: Creatv MicroTech, Inc.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  C07K16/28 ,  A61K39/395 ,  A61K45/06

Abstract: Methods of using detection of PD-L1 expression by circulating cancer cells in the screening, monitoring, treatment and diagnosis of cancer in subjects are disclosed. The methods are based on assaying one or more of circulating tumor cells (CTCs), epithelial to mesenchymal transition CTCs (EMTCTCs), cancer associated macrophage-like cells (CAMLs), and cancer associated vascular endothelial cells (CAVEs) isolated from a subject having cancer for PD-L1 expression.

公开(公告)号：US20180156801A1

公开(公告)日：2018-06-07

申请号：US15576472

申请日：2016-05-26

Applicant: CREATV MICROTECH INC.

Inventor： Daniel ADAMS ,  Cha-Mei TANG

IPC: G01N33/574 ,  G01N33/50

Abstract: Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.