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21.发明申请COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES 有权用于检测和分离磷酸化分子的组合物和方法公开(公告)号：US20070054304A1
公开(公告)日：2007-03-08
申请号：US11552275
申请日：2006-10-24
Applicant: Brian AGNEW , Joseph BEECHEM , Kyle GEE , Richard HAUGLAND , Thomas STEINBERG , Wayne PATTON
Inventor： Brian AGNEW , Joseph BEECHEM , Kyle GEE , Richard HAUGLAND , Thomas STEINBERG , Wayne PATTON
CPC classification number: G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
Abstract translation: 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与目标分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
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公开(公告)号：US20050069962A1
公开(公告)日：2005-03-31
申请号：US10467550
申请日：2002-10-02
Applicant: Robert Archer , Joseph Beechem , David Hagen , Richard Haugland , Rosaria Haugland
Inventor： Robert Archer , Joseph Beechem , David Hagen , Richard Haugland , Rosaria Haugland
IPC: G01N33/53
Abstract: The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
Abstract translation: 本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。 标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。 通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。 本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理 或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。 这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。
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公开(公告)号：US08304195B2
公开(公告)日：2012-11-06
申请号：US12047131
申请日：2008-03-12
Applicant: David Hagen , Joseph Beechem , Richard Haugland , Robert Archer , Rosaria Haugland
Inventor： David Hagen , Joseph Beechem , Richard Haugland , Robert Archer , Rosaria Haugland
IPC: G01N33/53
Abstract: The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.
Abstract translation: 本发明提供标记试剂和用于标记一抗的标记试剂和使用包含靶结合抗体和一种或多种标记试剂的免疫标记复合物检测样品中靶标的方法。 标记试剂包括单价抗体片段或非抗体单体蛋白质，由此标记的蛋白质对靶标结合抗体的特定区域具有亲和力，并且共价连接到标记物上。 通常，标记试剂是通过用抗体的Fc片段免疫山羊或兔而产生的抗Fc Fab或Fab'片段。 本发明提供标记试剂和免疫标记的复合物的离散子集，其有助于同时检测样品中的多个靶标，其中通过i）标记物与标记试剂的比率来区分免疫标记的复合物，或ii）物理 或iii）标记试剂与所述靶结合抗体的比例，或iv）所述靶结合抗体。 这对于可以连接到标记试剂和随后的用于检测多个靶的比例的免疫标记的复合物的荧光团标记物特别有用。
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公开(公告)号：US20110281740A1
公开(公告)日：2011-11-17
申请号：US13001545
申请日：2009-06-30
Applicant: Joseph Beechem , Vi-En Choong , Theo Nikiforov
Inventor： Joseph Beechem , Vi-En Choong , Theo Nikiforov
CPC classification number: C12Q1/686 , C12Q1/6869 , C12Q2565/629 , C12Q2565/301 , C12Q2565/101
Abstract: Provided herein are methods and compositions for real time single molecule sequencing of a polymeric molecule, such as a polynucleotide, by isolating the polymeric molecule in a nanofluidic device, subjecting it in situ to a polymerase reaction wherein various components of the polymerase reaction mixture are labeled, and determining the time-sequence of incorporation of monomeric subunits during the polymerization process.
Abstract translation: 本文提供了通过在纳米流体装置中分离聚合物分子来对聚合物分子例如多核苷酸进行实时单分子测序的方法和组合物，将其原位进行聚合酶反应，其中聚合酶反应混合物的各种组分被标记 ，并确定聚合过程中单体亚单位的掺入时间顺序。
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公开(公告)号：US20110136259A1
公开(公告)日：2011-06-09
申请号：US12961329
申请日：2010-12-06
Applicant: Bradley Love , Brian Dwyer , George Klee , Joseph Beechem , Ravinder Singh , Stefan Grebe
Inventor： Bradley Love , Brian Dwyer , George Klee , Joseph Beechem , Ravinder Singh , Stefan Grebe
IPC: G01N33/92
CPC classification number: G01N33/743 , G01N33/6854
Abstract: The present disclosure provides an immunoassay involving a multiplex of antibodies that recognize the same analyte but that have a different cross-reactivity to structurally similar compounds. Data obtained from the immunoassay involving observed analyte concentrations is input into an algorithm to determine the true concentration of the analyte in a sample.
Abstract translation: 本公开提供了涉及识别相同分析物但具有与结构上类似化合物不同的交叉反应性的抗体多重化的免疫测定。 从包括观察到的分析物浓度的免疫测定中获得的数据被输入到算法中以确定样品中分析物的真实浓度。
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Patent Agency Ranking
26.发明申请Compositions and methods for detection and isolation of phosphorylated molecules 审中-公开用于磷酸化分子检测和分离的组合物和方法公开(公告)号：US20110021372A1
公开(公告)日：2011-01-27
申请号：US12821003
申请日：2010-06-22
Applicant: Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
Inventor： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
IPC: C40B30/04 , C12Q1/68 , G01N33/68 , G01N33/566
CPC classification number: G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
Abstract translation: 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
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公开(公告)号：US20100233782A1
公开(公告)日：2010-09-16
申请号：US12576880
申请日：2009-10-09
Applicant: Federico Katzen , Julia Fletcher , Wieslaw Kudlicki , Joseph Beechem , Lilin Wang
Inventor： Federico Katzen , Julia Fletcher , Wieslaw Kudlicki , Joseph Beechem , Lilin Wang
Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.
Abstract translation: 提供的系统和方法用于产生通常不适于在体外表达系统中以可溶形式表达的目的蛋白质。 在一些方面，本发明提供了使用体外蛋白质合成系统合成蛋白质的方法，所述体外蛋白质合成系统包括诸如载脂蛋白或含有（“AAHC”）蛋白质的两亲性α螺旋的支架蛋白，其中产生比在 缺少支架蛋白。 支架蛋白质可以提供在与脂质相关的体外蛋白质合成系统中或与脂质不相关。 支架蛋白质可以作为蛋白质本身提供，或者可以由核酸模板编码并与感兴趣的蛋白质共表达。 本发明还提供用于合成可溶形式的蛋白质的组合物和试剂盒，其中组合物和试剂盒包括用于蛋白质表达和分离的细胞提取物。
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公开(公告)号：US07776533B2
公开(公告)日：2010-08-17
申请号：US11552275
申请日：2006-10-24
Applicant: Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
Inventor： Brian Agnew , Joseph Beechem , Kyle R Gee , Richard P Haugland , Thomas H Steinberg , Wayne F Patton
IPC: G01N33/53
CPC classification number: G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
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公开(公告)号：US20100035268A1
公开(公告)日：2010-02-11
申请号：US12528311
申请日：2008-02-21
Applicant: Joseph Beechem , Vi-En Choong , Theo Nikiforov
Inventor： Joseph Beechem , Vi-En Choong , Theo Nikiforov
IPC: C12Q1/68
CPC classification number: C12Q1/6827 , C12Q1/6874 , C12Q2565/518 , C12Q2565/101 , C12Q2563/113
Abstract: Provided herein are methods and compositions for real time single molecule sequencing of short nucleotide sequences using nucleotide fluorescent semiconductor nanocrystals-conjugated nucleotide primers.
Abstract translation: 本文提供了使用核苷酸荧光半导体纳米晶体共轭核苷酸引物对短核苷酸序列进行实时单分子测序的方法和组合物。
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30.发明申请COMPOSITIONS AND METHODS FOR DETECTION AND ISOLATION OF PHOSPHORYLATED MOLECULES 审中-公开用于检测和分离磷酸化分子的组合物和方法公开(公告)号：US20080044804A1
公开(公告)日：2008-02-21
申请号：US11697103
申请日：2007-04-05
Applicant: Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
Inventor： Brian AGNEW , Joseph Beechem , Kyle Gee , Richard Haugland , Thomas Steinberg , Wayne Patton
CPC classification number: G01N33/5008 , C12Q1/42 , C12Q1/485 , G01N33/502 , G01N33/5091 , G01N2333/4709 , Y10T436/142222 , Y10T436/145555 , Y10T436/147777 , Y10T436/201666
Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.
Abstract translation: 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。
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