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公开(公告)号:US20050037496A1
公开(公告)日:2005-02-17
申请号:US10899509
申请日:2004-07-26
申请人: David Rozema , Vladimir Budker , James Hagstrom , Vladimir Trubetskoy , Jon Wolff , Sean Monahan , Paul Slattum
发明人: David Rozema , Vladimir Budker , James Hagstrom , Vladimir Trubetskoy , Jon Wolff , Sean Monahan , Paul Slattum
CPC分类号: C12N15/88 , A61K47/58 , A61K47/59 , A61K47/645
摘要: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译: 为了向细胞递送核酸,将聚二聚物用于缩合的多核苷酸复合物中。 络合物可以用适量的正电荷和/或负电荷形成,使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
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公开(公告)号:US07470539B2
公开(公告)日:2008-12-30
申请号:US11256322
申请日:2005-10-21
申请人: Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
发明人: Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
IPC分类号: C12N15/88
CPC分类号: C12N15/87 , A61K47/6455 , C12N15/88
摘要: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译: 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成,或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物不稳定。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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公开(公告)号:US07098030B2
公开(公告)日:2006-08-29
申请号:US10899509
申请日:2004-07-26
申请人: David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
发明人: David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
IPC分类号: C12N15/88
CPC分类号: C12N15/88 , A61K47/58 , A61K47/59 , A61K47/645
摘要: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号:US07094605B2
公开(公告)日:2006-08-22
申请号:US11046598
申请日:2005-01-28
申请人: Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
发明人: Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
IPC分类号: C12N15/88
CPC分类号: C12N15/87 , A61K47/6455 , C12N15/88
摘要: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
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公开(公告)号:US20060159764A1
公开(公告)日:2006-07-20
申请号:US11322419
申请日:2005-12-30
申请人: Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
发明人: Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
CPC分类号: C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要: A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号:US20060063257A1
公开(公告)日:2006-03-23
申请号:US11256322
申请日:2005-10-21
申请人: Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
发明人: Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
IPC分类号: C12N15/87
CPC分类号: C12N15/87 , A61K47/6455 , C12N15/88
摘要: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译: 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成,或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物失稳。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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公开(公告)号:US20060024828A1
公开(公告)日:2006-02-02
申请号:US11235000
申请日:2005-09-26
申请人: Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
发明人: Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
CPC分类号: C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号:US20050123600A1
公开(公告)日:2005-06-09
申请号:US11046590
申请日:2005-01-28
申请人: Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
发明人: Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
CPC分类号: C12N15/88 , A61K47/60 , A61K47/645
摘要: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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