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公开(公告)号：US20050037496A1
公开(公告)日：2005-02-17
申请号：US10899509
申请日：2004-07-26
申请人： David Rozema , Vladimir Budker , James Hagstrom , Vladimir Trubetskoy , Jon Wolff , Sean Monahan , Paul Slattum
发明人： David Rozema , Vladimir Budker , James Hagstrom , Vladimir Trubetskoy , Jon Wolff , Sean Monahan , Paul Slattum
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译： 为了向细胞递送核酸，将聚二聚物用于缩合的多核苷酸复合物中。 络合物可以用适量的正电荷和/或负电荷形成，使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
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公开(公告)号：US20070036865A1
公开(公告)日：2007-02-15
申请号：US11533115
申请日：2006-09-19
申请人： David Rozema , Darren Wakefield , Jon Wolff , Vladimir Budker , Tatyana Budker , Sean Monahan , Vladimir Trubetskoy , James Hagstrom , Aaton Loomis , Paul Slattum
发明人： David Rozema , Darren Wakefield , Jon Wolff , Vladimir Budker , Tatyana Budker , Sean Monahan , Vladimir Trubetskoy , James Hagstrom , Aaton Loomis , Paul Slattum
摘要： We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
摘要翻译： 我们描述了pH敏感的内分泌溶解聚合物，包含pH敏感的内分解溶解聚合物的递送颗粒。 所述的颗粒能够将多核苷酸递送至来自外周循环的细胞，随后从内体释放。 内细胞分解的聚合物在细胞外是无活性的，但在暴露于酸化的内体室时破坏膜。
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公开(公告)号：US20060159764A1
公开(公告)日：2006-07-20
申请号：US11322419
申请日：2005-12-30
申请人： Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
发明人： Sean Monahan , David Rozema , Vladimir Trubetskoy , Paul Slattum , Jon Wolff , Vladimir Budker , James Hagstrom , Lisa Hanson
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US20070219363A1
公开(公告)日：2007-09-20
申请号：US11738146
申请日：2007-04-20
申请人： Vladimir Budker , Jon Wolff , Sean Monahan , Paul Slattum , James Hagstrom , Vladimir Trubetskoy , Tatyana Budker
发明人： Vladimir Budker , Jon Wolff , Sean Monahan , Paul Slattum , James Hagstrom , Vladimir Trubetskoy , Tatyana Budker
IPC分类号： C07D323/00 , C07C211/00 , C07H21/00
CPC分类号： C07D207/16
摘要： Chelator containing compounds are utilized in the delivery of molecules and polymers to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer. An ion is then added to the mixture thereby forming condensed polymer. In condensed form and in complex with the chelator, polymer can be delivered to a cell.
摘要翻译： 含螯合剂的化合物用于向动物细胞递送分子和聚合物。 至少一种螯合剂如冠醚连接到聚合物上，然后与另一种聚合物缔合。 然后将离子加入到混合物中，从而形成冷凝聚合物。 在浓缩形式和与螯合剂复合的情况下，聚合物可被递送至电池。
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公开(公告)号：US20050123600A1
公开(公告)日：2005-06-09
申请号：US11046590
申请日：2005-01-28
申请人： Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
发明人： Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jon Wolff , David Rozema , Sean Monahan
CPC分类号： C12N15/88 , A61K47/60 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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6.发明申请Expression of zeta negative and zeta positive nucleic acids using a dystrophin gene 审中-公开使用肌营养不良蛋白基因表达ζ阴性和ζ阳性核酸公开(公告)号：US20070173465A9
公开(公告)日：2007-07-26
申请号：US10339934
申请日：2003-01-10
申请人： Sean Monahan , Jon Wolff , Paul Slattum , James Hagstrom , Vladimir Budker , David Rozema
发明人： Sean Monahan , Jon Wolff , Paul Slattum , James Hagstrom , Vladimir Budker , David Rozema
CPC分类号： A61K48/0016 , A61K48/0041 , A61K48/0075 , A61K48/0083
摘要： Disclosed is expression of zeta negative and zeta positive nucleic acids or nucleic acid complexes using a dystrophin gene in a process for providing nucleic acid expression in a striated (skeletal or cardiac) muscle cell for the purpose of providing a change to the endogenous properties of the cell for cells affected by muscular dystrophy.
摘要翻译： 公开了在用于在条纹（骨骼或心脏）肌细胞中提供核酸表达的过程中使用肌营养不良蛋白基因的ζ阴性和ζ阳性核酸或核酸复合物的表达，以提供对 细胞受肌营养不良影响的细胞。
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公开(公告)号：US20050153451A1
公开(公告)日：2005-07-14
申请号：US10977025
申请日：2004-10-28
申请人： Jon Wolff , Vladimir Budker , James Hagstrom , Hans Herweijer , Julia Hegge , Paul Slattum , Sean Monahan , David Rozema
发明人： Jon Wolff , Vladimir Budker , James Hagstrom , Hans Herweijer , Julia Hegge , Paul Slattum , Sean Monahan , David Rozema
CPC分类号： A61K48/0083 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0016 , A61K48/0041 , A61K48/0075 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
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公开(公告)号：US20060024828A1
公开(公告)日：2006-02-02
申请号：US11235000
申请日：2005-09-26
申请人： Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
发明人： Vladimir Trubetskoy , Jon Wolff , Paul Slattum , Lisa Hanson , Vladimir Budker , James Hagstrom
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US20050170505A1
公开(公告)日：2005-08-04
申请号：US11046598
申请日：2005-01-28
申请人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
发明人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
CPC分类号： C12N15/87 , A61K47/6455 , C12N15/88
摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译： 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成，或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物不稳定。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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公开(公告)号：US20060063257A1
公开(公告)日：2006-03-23
申请号：US11256322
申请日：2005-10-21
申请人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
发明人： Darren Wakefield , David Rozema , Jon Wolff , Vladimir Trubetskoy , James Hagstrom , Vladimir Budker , Jason Klein , So Wong
IPC分类号： C12N15/87
CPC分类号： C12N15/87 , A61K47/6455 , C12N15/88
摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译： 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成，或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物失稳。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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