公开(公告)号：US10583440B2

公开(公告)日：2020-03-10

申请号：US16443629

申请日：2019-06-17

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC: G01N33/58 ,  G01N33/543 ,  B01L3/02 ,  B01F13/00 ,  B01F5/04 ,  B01F11/02 ,  B01L3/00 ,  B01F3/08 ,  C12Q1/6806 ,  C12Q1/686 ,  C40B40/06

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US10549279B2

公开(公告)日：2020-02-04

申请号：US15977805

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC: B01L3/00 ,  G01N1/00 ,  B01F5/04 ,  G01N33/543 ,  B01F3/08 ,  B01F13/00 ,  B01F11/02 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58 ,  C12Q1/686 ,  C40B40/06

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190367966A1

公开(公告)日：2019-12-05

申请号：US16375102

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: C12Q1/6806 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6804 ,  G01N33/50 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.

公开(公告)号：US20240408593A1

公开(公告)日：2024-12-12

申请号：US18664237

申请日：2024-05-14

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/31

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

公开(公告)号：US12128403B2

公开(公告)日：2024-10-29

申请号：US17873586

申请日：2022-07-26

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/31

CPC classification number: B01L3/502715 ,  B01L3/502723 ,  C12Q1/6837 ,  C12Q1/6841 ,  G01N1/312 ,  B01L2200/027 ,  B01L2300/0822 ,  B01L2400/0421

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

公开(公告)号：US11981960B1

公开(公告)日：2024-05-14

申请号：US17368259

申请日：2021-07-06

Applicant: 10x Genomics, Inc.

Inventor： Bill Kengli Lin ,  Jill Herschleb ,  Cedric Uytingco

IPC: C12Q1/6841 ,  C12Q1/6837

CPC classification number: C12Q1/6841 ,  C12Q1/6837

Abstract: Provided are methods of capturing an analyte from a biological sample using a hydrogel that includes capture probes to capture the analyte, identifying a region of interest of the biological sample, and isolating the region of interest from the biological sample by removing a portion of the hydrogel that corresponds to the region of interest. Compositions and kits for performing the methods are also provided.

公开(公告)号：US20230017773A1

公开(公告)日：2023-01-19

申请号：US17873586

申请日：2022-07-26

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  Augusto Manuel Tentori ,  Siyuan Xing ,  Rajiv Bharadwaj ,  Bill Kengli Lin ,  Felice Alessio Bava ,  Pratomo Putra Alimsijah ,  Nabil Mikhaiel

IPC: B01L3/00 ,  C12Q1/6837 ,  G01N1/31 ,  C12Q1/6841

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

公开(公告)号：US20220195420A1

公开(公告)日：2022-06-23

申请号：US17517282

申请日：2021-11-02

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Michael Schnall-Levin ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Pranav Patel ,  Katherine Pfeiffer ,  Andrew D. Price ,  Mohammad Rahimi Lenji ,  Tobias Daniel Wheeler ,  Yifeng Yin

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US20210372998A1

公开(公告)日：2021-12-02

申请号：US17209045

申请日：2021-03-22

Applicant: 10X Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

公开(公告)号：US10898900B2

公开(公告)日：2021-01-26

申请号：US16812866

申请日：2020-03-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Anthony Makarewicz ,  Bill Kengli Lin

IPC: B01L3/00 ,  B01F5/04 ,  G01N33/543 ,  G01N33/58 ,  B01F3/08 ,  B01F13/00 ,  B01F11/02 ,  B01L3/02 ,  C12Q1/6806 ,  C12Q1/686 ,  C40B40/06

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.