公开(公告)号：US11467153B2

公开(公告)日：2022-10-11

申请号：US17393107

申请日：2021-08-03

申请人： 10x Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC分类号： G01N33/532 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6881 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12N15/11 ,  G01N33/569 ,  C40B70/00 ,  C40B30/04

摘要： The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US20220170075A1

公开(公告)日：2022-06-02

申请号：US17369698

申请日：2021-07-07

申请人： 10X Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Jason Bell

IPC分类号： C12Q1/6806

摘要： Provided herein are methods, compositions, and systems for transposon loading. Transposons are loaded with nucleic acid molecules, allowing for transposition reactions of cellular nucleic acids. The present invention may improve transposon loading, yield of productive fragments, while minimizing potential nucleic acid fragment loss or cross-contamination.

公开(公告)号：US20210102936A1

公开(公告)日：2021-04-08

申请号：US16897126

申请日：2020-06-09

申请人： 10X Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20190383798A1

公开(公告)日：2019-12-19

申请号：US16375110

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： G01N33/50 ,  G01N33/543 ,  G01N33/53 ,  G01N33/569 ,  C12N15/10

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

公开(公告)号：US11739440B2

公开(公告)日：2023-08-29

申请号：US16897126

申请日：2020-06-09

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Katherine Pfeiffer ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12N15/11 ,  C40B70/00 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/569

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/505 ,  G01N33/5032 ,  G01N33/532 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/548 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20230236175A1

公开(公告)日：2023-07-27

申请号：US17820560

申请日：2022-08-17

申请人： 10x Genomics, Inc.

发明人： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC分类号： G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

CPC分类号： G01N33/532 ,  C07K14/70539 ,  C12N15/85 ,  G01N33/54306 ,  G01N33/548 ,  C12N15/1037 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/505 ,  G01N33/5308 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/5304 ,  G01N33/5306 ,  C12N15/1055 ,  C12N15/1065 ,  G01N33/5032 ,  G01N33/54366 ,  G01N33/56977 ,  C12Q2537/164 ,  C12Q2565/1015 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12N2310/20 ,  C40B70/00

摘要： The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US20200263232A1

公开(公告)日：2020-08-20

申请号：US16530930

申请日：2019-08-02

申请人： 10X GENOMICS, INC.

发明人： Jason Bell ,  Josephine Lee ,  Corey Nemec ,  Francesca Meschi

IPC分类号： C12Q1/6806 ,  C12N9/12 ,  C12N15/10

摘要： Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3′-terminated blocking group or sequences that prevent amplification or extension.

公开(公告)号：US12065688B2

公开(公告)日：2024-08-20

申请号：US16434095

申请日：2019-06-06

申请人： 10X GENOMICS, INC.

发明人： Jason Bell

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806 ,  C12Y301/31001

摘要： Provided herein are compositions and methods for cellular analysis. Nucleic acid from single cells may be processed in one or more partitions. A partition may comprise a cell bead and one or more enzymes for nucleic acid processing. A partition may comprise a functionalized polymer. In some cases, single cells may be subjected to epigenetic analysis, thereby generating an epigenetic profile for each cell from a plurality of cells.

公开(公告)号：US12049712B2

公开(公告)日：2024-07-30

申请号：US17229859

申请日：2021-04-13

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20230383440A1

公开(公告)日：2023-11-30

申请号：US18231460

申请日：2023-08-08

申请人： 10x Genomics, Inc.

发明人： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C07K14/74 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/85 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/548 ,  C12N15/1037 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/505 ,  G01N33/5308 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/5304 ,  G01N33/5306 ,  C12N15/1055 ,  C12N15/1065 ,  G01N33/5032 ,  G01N33/54366 ,  G01N33/56977 ,  C12Q2537/164 ,  C12Q2565/1015 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12N2310/20 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes.