公开(公告)号：US20240287600A1

公开(公告)日：2024-08-29

申请号：US18596867

申请日：2024-03-06

申请人： 10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer ,  Tarjei Sigurd Mikkelsen

IPC分类号： C12Q1/6874 ,  C12Q1/6841

CPC分类号： C12Q1/6874 ,  C12Q1/6841 ,  C12Q2525/161 ,  C12Q2565/519

摘要： Provided herein are methods of determining a location of a biological analyte in a biological sample.

公开(公告)号：US20220119869A1

公开(公告)日：2022-04-21

申请号：US17565081

申请日：2021-12-29

申请人： 10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer ,  Zachary Bent ,  Yifeng Yin ,  Eileen Dalin

IPC分类号： C12Q1/6837 ,  C12Q1/6841

摘要： Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample that include the use of a blocking probe.

公开(公告)号：US20210317524A1

公开(公告)日：2021-10-14

申请号：US17272033

申请日：2019-08-27

申请人： 10x Genomics, Inc.

发明人： Michael Ybarra Lucero ,  Michael Schnall-Levin ,  Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Paul Giresi ,  Benjamin Hindson

IPC分类号： C12Q1/6874

摘要： Methods for determining a location of a feature in a spatial array of features include: providing a spatial array including a plurality of features on a substrate, where a feature of the plurality of features has a capture probe, and where the capture probe has a spatial barcode and a constant sequence; hybridizing a first sequencing probe to the spatial barcode, where the first sequencing probe has a first label and a first nucleotide sequence; obtaining a first image of the first label with the first sequencing probe hybridized to the spatial barcode; determining, based on the first image, a first portion of a sequence of the spatial barcode; and associating the feature with a location in the spatial array based on a location of the first label in the first image.

公开(公告)号：US11891654B2

公开(公告)日：2024-02-06

申请号：US17184117

申请日：2021-02-24

申请人： 10x Genomics, Inc.

发明人： Luigi Jhon Alvarado Martinez ,  Eswar Prasad Ramachandran Iyer

IPC分类号： C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/6827

CPC分类号： C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2543/101 ,  C12Q2565/514

摘要： Provided herein are methods of determining a location of a target nucleic acid in a biological sample.

公开(公告)号：US11827935B1

公开(公告)日：2023-11-28

申请号：US17531093

申请日：2021-11-19

申请人： 10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer ,  Yifeng Yin ,  Sultan Doganay Tuncer

IPC分类号： C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

CPC分类号： C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6862

摘要： Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.

公开(公告)号：US11753675B2

公开(公告)日：2023-09-12

申请号：US17974226

申请日：2022-10-26

申请人： 10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer

IPC分类号： C12Q1/6837

CPC分类号： C12Q1/6837

摘要： The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

公开(公告)号：US20230126825A1

公开(公告)日：2023-04-27

申请号：US18087440

申请日：2022-12-22

申请人： 10x Genomics, Inc.

发明人： Monica Nagendran ,  Jonathan Cheng ,  Eswar Prasad Ramachandran Iyer

IPC分类号： C12Q1/6841

摘要： Provided herein are methods for determining the mislocalization of an analyte by capturing the analyte on an array and measuring the mislocalization distance.

公开(公告)号：US11434524B2

公开(公告)日：2022-09-06

申请号：US17565081

申请日：2021-12-29

申请人： 10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer ,  Zachary Bent ,  Yifeng Yin ,  Eileen Dalin

IPC分类号： C12Q1/6837 ,  C12Q1/6841

摘要： Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample that include the use of a blocking probe.

公开(公告)号：US20220276229A1

公开(公告)日：2022-09-01

申请号：US17680209

申请日：2022-02-24

申请人： 10X GENOMICS, INC.

发明人： Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Andrew Kohlway ,  Luigi Jhon Alvarado Martinez ,  Katherine Pfeiffer ,  Andrew D. Price

IPC分类号： G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

摘要： The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.

公开(公告)号：US20220145370A1

公开(公告)日：2022-05-12

申请号：US17480724

申请日：2021-09-21

申请人： 10x Genomics, Inc.

发明人： Luigi Jhon Alvarado Martinez ,  Eswar Prasad Ramachandran Iyer ,  Jason Bell

IPC分类号： C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874

摘要： Provided herein are methods, compositions, and systems for processing nucleic acids from individual cells or cell populations. Cells are co-partitioned and processed, allowing for analysis of a variety of types of RNA from cells while minimizing ribosomal RNA species. The present invention enables high-throughput measurement of full length RNA from single cells with a streamlined workflow.