公开(公告)号：US20190119713A1

公开(公告)日：2019-04-25

申请号：US16018400

申请日：2018-06-26

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Che MA

IPC: C12P19/14 ,  C07K16/28 ,  A61K45/06 ,  C07K16/32 ,  A61K39/395 ,  C12N9/24 ,  C07K16/10 ,  C07K16/00 ,  A61K39/42 ,  C07K16/30 ,  C07K16/18 ,  C07K16/24

Abstract: The present disclosure relates to glycoproteins, particularly monoclonal antibodies, comprising a glycoengineered Fc region, wherein said Fc region comprises an optimized N-glycan having the structure of Sia2(α2-6)Gal2GlcNAc2Man3GlcNAc2. The glycoengineered Fc region binds FcγRIIA or FcγRIIIA with a greater affinity, relative to comparable monoclonal antibodies comprising the wild-type Fc region. The monoclonal antibodies of the invention are particularly useful in preventing, treating, or ameliorating one or more symptoms associated with a disease, disorder, or infection where an enhanced efficacy of effector cell function (e.g., ADCC) mediated by FcγR is desired, e.g., cancer, autoimmune, infectious disease, and in enhancing the therapeutic efficacy of therapeutic antibodies the effect of which is mediated by ADCC.

公开(公告)号：US20170362265A1

公开(公告)日：2017-12-21

申请号：US15453836

申请日：2017-03-08

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Sachin S. SHIVATARE

IPC: C07H5/06 ,  A61K31/702 ,  C12N9/10 ,  C07K16/10 ,  C12P19/28

CPC classification number: C07H5/06 ,  A61K31/702 ,  C07K16/1045 ,  C08B37/006 ,  C12N9/107 ,  C12N2740/16063 ,  C12P19/04 ,  C12P19/28 ,  G01N33/50 ,  G01N33/532 ,  G01N33/56988 ,  G01N33/68 ,  G01N33/6893

Abstract: The present disclosure relates to novel modular methods for generating a diversity of N-glycans of high mannose, hybrid and complex types. The present disclosure also relates to exemplary arrays of the synthesized N-glycans spotted onto aluminium oxide coated slides. These arrays can be used to detect and analyze binding interactions between the synthesized N-glycans and glycan binding molecules, such as HIV-1 neutralizing antibodies. The present disclosure also relates to methods for identifying agents that bind to various types of molecules on the arrays and to defining the structural elements of the molecules on the arrays that bind to those agents. The arrays and methods provided herein may be used for general epitope identification, drug discovery and as analytical tools. The present disclosure also provides useful glycans and epitope determinants that are useful in detecting, diagnosing, recurrence monitoring and preventing pathological diseases such as HIV.

公开(公告)号：US20160230201A1

公开(公告)日：2016-08-11

申请号：US15003362

申请日：2016-01-21

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Tsung-I TSAI ,  Chung-Yi WU

IPC: C12P19/18 ,  C12P19/04

Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.

Abstract translation: 一种新型的UDP-Gal再生过程及其与半乳糖基转移酶的组合使用，以将半乳糖加入合适的受体底物。 本文还描述了大规模生产Globo系列寡糖的合成方法，其中所述方法可涉及糖基转移酶反应和核苷酸糖再生过程的组合。

公开(公告)号：US20160175421A1

公开(公告)日：2016-06-23

申请号：US14392333

申请日：2014-06-27

Applicant: Chi-Huey WONG ,  Chung-Yi WU ,  Chia-Hung WANG ,  Shiou-Ting LI ,  ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Chia-Hung WANG ,  Shiou-Ting LI

IPC: A61K39/095 ,  A61K39/385 ,  A61K39/39 ,  C07H15/04

CPC classification number: A61K39/095 ,  A61K31/715 ,  A61K39/385 ,  A61K39/39 ,  A61K2039/55505 ,  A61K2039/55511 ,  A61K2039/55566 ,  A61K2039/55577 ,  A61K2039/6037 ,  A61K2039/627 ,  C07H1/00 ,  C07H15/04 ,  C07H15/18 ,  C08B37/006

Abstract: Described herein are synthetic glycan conjugates comprising a carrier and a glycan moiety derived from Neisseria meningitidis, wherein the glycan moiety is covalently linked to the carrier through a linker. Also provided herein are a mixture of the glycan conjugates thereof, immunogenic compositions thereof, and kits thereof. The invention further provides methods of using the synthetic glycan conjugates and immunogenic compositions thereof to treat and/or reduce the risk of infectious diseases such as bacterial infections.

Abstract translation: 本文描述的是包含载体和衍生自脑膜炎奈瑟氏球菌的聚糖部分的合成聚糖缀合物，其中所述聚糖部分通过连接体共价连接至所述载体。 本文还提供其聚糖缀合物，其免疫原性组合物及其试剂盒的混合物。 本发明还提供使用合成聚糖缀合物及其免疫原性组合物治疗和/或降低感染性疾病如细菌感染的风险的方法。

公开(公告)号：US20160166661A1

公开(公告)日：2016-06-16

申请号：US14906663

申请日：2014-07-26

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Hsien-Yeh HSU ,  Shih-Fen LIAO ,  Chi-Hui LIANG

IPC: A61K39/00

CPC classification number: A61K39/0002 ,  A61K36/074 ,  A61K39/0011 ,  A61K2039/55572 ,  A61K2039/575

Abstract: Immunogenic compositions, cancer vaccines and methods for treating cancer comprising FMS, the fucose-enriched polysaccharide fraction from Reishi F3, are provided. Compositions comprise fucose-enriched Reishi polysaccharide fraction (FMS) MW=˜35 kDa, wherein the FMS is isolated by size-exclusion chromatography from Reishi F3, and the FMS comprises polysaccharides having primarily a backbone selected from 1,4-mannan and 1,6-α-galactan, wherein the backbone is linked to a terminal fucose-containing side-chain Immunogenic compositions comprising glycolipid adjuvants are provided. Antibodies generated by immunogenic compositions disclosed herein bind cancer cells comprising antigens Globo H, Globo H, Gb3, Gb4, Gb5 (SSEA-3) and SSEA-4 on the cell surface.

Abstract translation: 提供免疫原性组合物，癌症疫苗和治疗癌症的方法，其包括FMS，来自Reishi F3的岩藻糖富集的多糖部分。 组合物包含岩藻糖富集的赖斯多糖级分（FMS）MW =〜35kDa，其中通过来自Reishi F3的尺寸排阻色谱法分离FMS，并且FMS包含主要选自1,4-甘露聚糖和1， 6-α-半乳聚糖，其中所述主链与包含末端岩藻糖的侧链连接，提供包含糖脂佐剂的免疫原性组合物。 由本文公开的免疫原性组合物产生的抗体结合细胞表面上包含抗原Globo H，Globo H，Gb 3，Gb 4，Gb 5（SSEA-3）和SSEA-4的癌细胞。