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公开(公告)号：US08822174B1
公开(公告)日：2014-09-02
申请号：US13840509
申请日：2013-03-15
IPC分类号： C12Q1/22
摘要： A sterilization indicator for oxidative sterilants, comprising a first compartment comprising spores of one or more microorganism species, wherein the spores have been pretreated with and comprises a compound comprising a transition metal ion that is reactive with an oxidative sterilant; a second compartment comprising a growth medium and adapted to combine contents of the first compartment with contents of the second compartment for incubation after the sterilization indicator has been exposed to an oxidative sterilant; and an agent disposed in the growth medium and selected to indicate viability of the spores after the sterilization indicator has been exposed to the oxidative sterilant.
摘要翻译： 一种用于氧化灭菌剂的消毒指示剂，其包含由一种或多种微生物种类的孢子构成的第一隔室，其中所述孢子已经用包含与氧化灭菌剂反应的过渡金属离子预处理并包含其中的化合物; 第二隔室，包括生长介质，并且适于将所述第一隔室的内容物与所述第二隔室的内容物组合，以在所述灭菌指示剂暴露于氧化灭菌剂之后进行孵育; 以及设置在生长培养基中并选择用于指示灭菌指示剂暴露于氧化灭菌剂之后的孢子的活力的试剂。
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公开(公告)号：US10900062B2
公开(公告)日：2021-01-26
申请号：US15699220
申请日：2017-09-08
IPC分类号： C12Q1/22 , G01N27/12 , C12M1/12 , G01N33/497
摘要： A process for determining the viability of a biological indicator includes exposing the biological indicator to a viability detection medium, the biological indicator including test microorganisms, the exposing the biological indicator to the viability detection medium producing a gaseous reaction product when one or more of the test microorganisms are viable. The presence or absence of the gaseous reaction product produced by the biological indicator combined with the viability detection medium is detected with a sensing device, the sensing device comprising a resistive sensor, wherein the presence of the gaseous reaction product indicates the presence of viable test microorganisms and the absence of the gaseous reaction product indicates the absence of viable test microorganisms. A sterilization detection device includes a container configured to contain the biological indicator, a viability detection medium, and the sensing device.
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公开(公告)号：US20180273957A1
公开(公告)日：2018-09-27
申请号：US16001203
申请日：2018-06-06
IPC分类号： C12N15/75 , C12Q1/689 , A61L2/28 , C12M1/12 , C12Q1/6897 , C12M1/34 , C12Q1/22 , C12Q1/34 , C12N9/38
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20170275632A1
公开(公告)日：2017-09-28
申请号：US15618157
申请日：2017-06-09
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20170275631A1
公开(公告)日：2017-09-28
申请号：US15618156
申请日：2017-06-09
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US09701968B2
公开(公告)日：2017-07-11
申请号：US15204124
申请日：2016-07-07
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160312263A1
公开(公告)日：2016-10-27
申请号：US15204124
申请日：2016-07-07
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160102335A1
公开(公告)日：2016-04-14
申请号：US14512708
申请日：2014-10-13
CPC分类号： C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
摘要： This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US09303283B2
公开(公告)日：2016-04-05
申请号：US14090116
申请日：2013-11-26
摘要： A combined sterilization indicator incubator and reader system, including a sterilization indicator vial containing one of a variety of selected biological indicators and a liquid, at least two incubator blocks, each incubator block independently operable to incubate the sterilization indicator vial at a plurality of independently selectable temperatures, each of the incubator blocks including at least one heating element operable to heat the incubator block to any one of the plurality of independently selectable temperatures; a light source, a photodetector and a control system configured to operate the combined system to determine the efficacy of a variety of sterilization processes. The system calculates and compares a slope to a predetermined threshold slope for the biological indicator, and provides output based only on the comparison of the calculated slope to the predetermined threshold slope without first determining either a baseline or a minimum value of the output from the photodetector.
摘要翻译： 一种组合的灭菌指示器培养箱和读取器系统，包括含有多种选择的生物指示剂中的一种的灭菌指示剂小瓶和液体的至少两个培养箱块，每个培养箱块可独立操作以将灭菌指示剂小瓶以多个可独立选择的温度 每个孵化器块包括至少一个加热元件，其可操作以将培养箱块加热到多个可独立选择的温度中的任何一个; 光源，光电检测器和控制系统，其被配置为操作组合系统以确定各种灭菌过程的功效。 该系统计算并比较生物学指示器的斜率与预定阈值斜率，并且仅基于所计算的斜率与预定阈值斜率的比较来提供输出，而不首先确定来自光电探测器的输出的基线或最小值 。
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30.发明授权Non-enzyme based detection method for electronic monitoring of biological indicator 有权用于电子监测生物指示剂的非酶检测方法公开(公告)号：US09121050B2
公开(公告)日：2015-09-01
申请号：US13836787
申请日：2013-03-15
CPC分类号： C12Q1/22 , B01L2300/0609 , B01L2300/0816 , C12Q1/001 , C12Q1/006 , C12Q1/6816 , C12Q2527/125
摘要： A sterilization indicator system and method of using the system to determine efficacy of a sterilization process. The system may include a vial having an optional first compartment and a second compartment comprising a growth medium comprising one or more of a disaccharide, an oligosaccharide or a polysaccharide capable of conversion to a monosaccharide by germinating spores of the one or more species of microorganism, the vial being free of the monosaccharide prior to use; a strip including two or more electrodes to oxidize the monosaccharide and to carry a resulting electrical signal, and an apparatus to detect and measure the electrical signal resulting from the oxidation. Spores of a suitable biological indicator may be disposed in the first compartment and/or on the strip.
摘要翻译： 灭菌指示器系统和使用该系统来确定灭菌过程的功效的方法。 该系统可以包括具有任选的第一隔室的小瓶和包含生长培养基的第二隔室，所述生长培养基包含一种或多种能够通过发芽一种或多种微生物的孢子转化成单糖的二糖，寡糖或多糖， 使用前小瓶不含单糖; 包括两个或更多个电极以氧化单糖并携带所得电信号的条带，以及用于检测和测量由氧化产生的电信号的装置。 合适的生物指示剂的孢子可以设置在第一隔室和/或条带上。
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