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公开(公告)号：US20100113846A1
公开(公告)日：2010-05-06
申请号：US12560370
申请日：2009-09-15
CPC分类号： C12P5/007 , C07C1/2078 , C07C1/24 , C12N9/14 , C12N9/16 , C12N9/88 , C12N9/90 , C12Y301/03 , C12Y301/07 , C12Y306/01 , C12Y503/03002
摘要： The present invention provides methods for producing derivatives from cultured cells. In addition, the present invention provides methods for conversion of prenyl deerivatives, obtained from biological or petrochemical sources, to isoprene by employing chemical or biological catalysts. The present invention also provides compositions that include the cultured cells or isoprene or prenyl derivatives produced there from.
摘要翻译： 本发明提供了从培养细胞产生衍生物的方法。 此外，本发明提供了通过使用化学或生物催化剂将从生物或石油化学来源获得的异戊烯基脱戊基转化为异戊二烯的方法。 本发明还提供了包含从其制备的培养细胞或异戊二烯或异戊二烯基衍生物的组合物。
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公开(公告)号：US08901262B2
公开(公告)日：2014-12-02
申请号：US13971484
申请日：2013-08-20
CPC分类号： C08F4/52 , C08F4/16 , C08F36/08 , C12P5/007 , Y02E50/343
摘要： This invention relates to compositions and methods for producing polymers of isoprene derived from renewable resources, such as isoprene produced from cultured cells that use renewable carbon sources. A starting isoprene composition, such as a bioisoprene composition, is distinguished from petroleum based isoprene by its purity profile (such as lower levels of certain C5 hydrocarbons other than isoprene, presence of certain compounds associated with the biological process for production) and the relative content of the carbon isotopes. Polymers obtained by polymerization of such starting isoprene composition according to this invention, such as a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene, are distinguishable from isoprene containing polymers from petrochemical resources.
摘要翻译： 本发明涉及用于生产衍生自可再生资源的异戊二烯聚合物的组合物和方法，例如由使用可再生碳源的培养细胞产生的异戊二烯。 起始的异戊二烯组合物，例如生物异戊二烯组合物，其特征在于其基于石油的异戊二烯的纯度分布（例如除了异戊二烯之外的某些C5烃的较低水平，与生产生物过程相关的某些化合物的存在）以及相对含量 的碳同位素。 通过聚合本发明的这种起始异戊二烯组合物得到的聚合物，例如聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物，与来自石化资源的含异戊二烯聚合物是可区分的。
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公开(公告)号：US20110040058A1
公开(公告)日：2011-02-17
申请号：US12817093
申请日：2010-06-16
IPC分类号： C08F136/08 , C12P5/02 , C12P5/00
CPC分类号： C08F4/52 , C08F4/16 , C08F36/08 , C12P5/007 , Y02E50/343
摘要： The invention features compositions and methods for producing polymers of isoprene derived from renewable resources such as isoprene produced from cultured cells that use renewable carbon sources. A starting isoprene composition, such as a bioisoprene composition, is distinguished from petroleum based isoprene by the purity profile (such as lower levels of certain C5 hydrocarbons other than isoprene, presence of certain compounds associated with the biological process for production) and the relative content of the carbon isotopes. Polymers obtained by polymerization of such starting isoprene composition according to this invention, such as a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene, are distinguishable from isoprene containing polymers from petrochemical resources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber. Also provided are methods for verifying that a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene contains isoprene that is from a sustainable renewable non-petroleum derived source.
摘要翻译： 本发明的特征是用于生产衍生自可再生资源的异戊二烯聚合物的组合物和方法，例如由使用可再生碳源的培养细胞产生的异戊二烯。 起始的异戊二烯组合物如生物异戊二烯组合物通过纯度分布（例如除异戊二烯以外的某些C5烃的较低水平，与生产生物过程相关的某些化合物的存在）以及相对含量 的碳同位素。 通过聚合本发明的这种起始异戊二烯组合物得到的聚合物，例如聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物，与来自石化资源的含异戊二烯聚合物是可区分的。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22‰。 这种类型的聚异戊二烯可以是顺式-1,4-聚异戊二烯均聚物橡胶。 还提供了用于验证聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物包含来自可持续可再生非石油衍生源的异戊二烯的方法。
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公开(公告)号：US20140080983A1
公开(公告)日：2014-03-20
申请号：US13971484
申请日：2013-08-20
CPC分类号： C08F4/52 , C08F4/16 , C08F36/08 , C12P5/007 , Y02E50/343
摘要： This invention relates to compositions and methods for producing polymers of isoprene derived from renewable resources, such as isoprene produced from cultured cells that use renewable carbon sources. A starting isoprene composition, such as a bioisoprene composition, is distinguished from petroleum based isoprene by its purity profile (such as lower levels of certain C5 hydrocarbons other than isoprene, presence of certain compounds associated with the biological process for production) and the relative content of the carbon isotopes. Polymers obtained by polymerization of such starting isoprene composition according to this invention, such as a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene, are distinguishable from isoprene containing polymers from petrochemical resources.
摘要翻译： 本发明涉及用于生产衍生自可再生资源的异戊二烯聚合物的组合物和方法，例如由使用可再生碳源的培养细胞产生的异戊二烯。 起始的异戊二烯组合物，例如生物异戊二烯组合物，其特征在于其石油基异戊二烯的纯度分布（例如除了异戊二烯之外的某些C5烃的较低水平，与生产生物过程相关的某些化合物的存在）以及相对含量 的碳同位素。 通过聚合本发明的这种起始异戊二烯组合物得到的聚合物，例如聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物，与来自石化资源的含异戊二烯聚合物是可区分的。
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公开(公告)号：US08546506B2
公开(公告)日：2013-10-01
申请号：US12817093
申请日：2010-06-16
IPC分类号： C08F236/08 , C07C11/18 , C12P5/02
CPC分类号： C08F4/52 , C08F4/16 , C08F36/08 , C12P5/007 , Y02E50/343
摘要： The invention features compositions and methods for producing polymers of isoprene derived from renewable resources such as isoprene produced from cultured cells that use renewable carbon sources. A starting isoprene composition, such as a bioisoprene composition, is distinguished from petroleum based isoprene by the purity profile (such as lower levels of certain C5 hydrocarbons other than isoprene, presence of certain compounds associated with the biological process for production) and the relative content of the carbon isotopes. Polymers obtained by polymerization of such starting isoprene composition according to this invention, such as a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene, are distinguishable from isoprene containing polymers from petrochemical resources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber. Also provided are methods for verifying that a polyisoprene homopolymer or a copolymer having repeat units that are derived from isoprene contains isoprene that is from a sustainable renewable non-petroleum derived source.
摘要翻译： 本发明的特征是用于生产衍生自可再生资源的异戊二烯聚合物的组合物和方法，例如由使用可再生碳源的培养细胞产生的异戊二烯。 起始的异戊二烯组合物如生物异戊二烯组合物通过纯度分布（例如除异戊二烯以外的某些C5烃的较低水平，与生产生物过程相关的某些化合物的存在）以及相对含量 的碳同位素。 通过聚合本发明的这种起始异戊二烯组合物得到的聚合物，例如聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物，与来自石化资源的含异戊二烯聚合物是可区分的。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-220 / 00。 这种类型的聚异戊二烯可以是顺式-1,4-聚异戊二烯均聚物橡胶。 还提供了用于验证聚异戊二烯均聚物或具有衍生自异戊二烯的重复单元的共聚物包含来自可持续可再生非石油衍生源的异戊二烯的方法。
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公开(公告)号：US08450549B2
公开(公告)日：2013-05-28
申请号：US12818090
申请日：2010-06-17
摘要： The invention provides for methods, compositions and systems using bioisoprene derived from renewable carbon for production of a variety of hydrocarbon fuels and fuel additives.
摘要翻译： 本发明提供了使用衍生自可再生碳的生物异戊二烯用于生产各种烃燃料和燃料添加剂的方法，组合物和系统。
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公开(公告)号：US20110046422A1
公开(公告)日：2011-02-24
申请号：US12818090
申请日：2010-06-17
摘要： The invention provides for methods, compositions and systems using bioisoprene derived from renewable carbon for production of a variety of hydrocarbon fuels and fuel additives.
摘要翻译： 本发明提供了使用衍生自可再生碳的生物异戊二烯用于生产各种烃燃料和燃料添加剂的方法，组合物和系统。
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公开(公告)号：US4568647A
公开(公告)日：1986-02-04
申请号：US540975
申请日：1983-10-11
申请人： Karl J. Sanford
发明人： Karl J. Sanford
CPC分类号： G01N33/6839
摘要： Disclosed herein is an assay for determination of albumin in a liquid sample. This assay involves the use of a dye of the structure: ##STR1## wherein n is zero or a positive integer up to 3; R.sup.1, R.sup.2 and R.sup.3 are independently hydrogen, alkyl, cycloalkyl, aryl, alkoxy, aroxy, hydroxy, carboxy, alkoxycarbonyl, amino or a nonaromatic heterocyclic group; and Z and Z.sub.1 independently represent the carbon, nitrogen, oxygen or sulfur atoms needed to complete a substituted or unsubstituted 5- to 7-membered carbocyclic or heterocyclic ring. This dye has a high affinity for albumin over a wide pH range. When the dye is bound to albumin, a shift in the dye spectral absorption occurs. Analytical elements containing this dye and an assay method are disclosed herein.
摘要翻译： 本文公开了用于测定液体样品中的白蛋白的测定法。 该测定涉及使用以下结构的染料：其中n为零或最大为3的正整数; R1，R2和R3独立地是氢，烷基，环烷基，芳基，烷氧基，羟基，羟基，羧基，烷氧基羰基，氨基或非芳族杂环基; 并且Z和Z1独立地表示完成取代或未取代的5-至7-元碳环或杂环所需的碳，氮，氧或硫原子。 该染料在宽pH范围内对白蛋白具有高亲和力。 当染料与白蛋白结合时，染料光谱吸收发生变化。 包含该染料的分析元素和测定方法在本文中公开。
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29.发明授权Incorporation of pyridoxal phosphate in dry analytical elements for the determination of enzymes 失效在干燥分析元素中掺入磷酸吡哆醛用于测定酶
公开(公告)号：US4450232A
公开(公告)日：1984-05-22
申请号：US345844
申请日：1982-02-04
CPC分类号： C12Q1/48 , Y10S435/805
摘要： Enzymes which are activated by pyridoxal phosphate are assayed in a dry analytical element which includes a high coverage of pyridoxal phosphate. The coverage of pyridoxal phosphate is such that the multiplication product of the coverage and the spreading coefficient of the element results in a reagent mixture concentration of at least about 0.9 mmole per liter when a liquid sample is contacted with the element thereby eliminating the need for a preincubation of the sample with a solution containing pyridoxal phosphate. The reactions for the determination of the enzymes are initiated by contact of the dry elements with a liquid sample.
摘要翻译： 通过磷酸吡哆醛激活的酶在包含磷酸吡哆醛的高分辨率的干燥分析元件中进行测定。 吡哆醛磷酸盐的覆盖范围使得当液体样品与元件接触时，覆盖率和元件铺展系数的乘积导致试剂混合物浓度至少为约0.9毫摩尔/升，从而不需要 用含有吡哆醛磷酸盐的溶液预培养样品。 通过干燥元件与液体样品的接触来引发酶的测定反应。
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公开(公告)号：US4250255A
公开(公告)日：1981-02-10
申请号：US814770
申请日：1977-07-11
申请人： Karl J. Sanford
发明人： Karl J. Sanford
CPC分类号： C12Q1/00
摘要： The activity of an isoenzyme in a sample is determined by assaying for the isoenzyme in the presence of a predetermined concentration of an ionic amphiphile having a hydrophilic and a hydrophobic portion that has a discriminating effect on the activity of the isoenzyme and determining the activity of the isoenzyme present in the sample.
摘要翻译： 通过在预定浓度的亲水和疏水部分的离子两亲物的存在下测定同位素来测定样品中的同功酶的活性，所述亲水和疏水部分对同工酶的活性具有区别作用并确定 样品中存在同工酶。
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