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21.发明申请COMPOSITIONS AND METHODS FOR THE TARGETED INSERTION OF A NUCLEOTIDE SEQUENCE OF INTEREST INTO THE GENOME OF A PLANT 有权有针对性地插入植物基因组的核苷酸序列的组合物和方法公开(公告)号：US20080209595A1
公开(公告)日：2008-08-28
申请号：US12039307
申请日：2008-02-28
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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22.发明授权公开(公告)号：US07102055B1
公开(公告)日：2006-09-05
申请号：US09455051
申请日：1999-12-06
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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公开(公告)号：US06197561B1
公开(公告)日：2001-03-06
申请号：US09118442
申请日：1998-07-17
IPC分类号： C12N900
CPC分类号： C12N9/1205 , C12N9/16 , C12N9/90 , C12N15/8243
摘要： This invention relates to newly identified polynucleotides and polypeptides, variants and derivatives of same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the phytate metabolic pathway.
摘要翻译： 本发明涉及新鉴定的多核苷酸和多肽，其变体和衍生物; 制备多核苷酸，多肽，变体，衍生物和拮抗剂的方法。 特别地，本发明涉及植酸代谢途径的多核苷酸和多肽。
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24.发明授权公开(公告)号：US09222098B2
公开(公告)日：2015-12-29
申请号：US10639751
申请日：2003-08-12
IPC分类号： C12N15/82 , C12N9/00 , C12N15/10 , C12N15/113 , C12N15/90
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site. The second recombination site is shared between the first and the second transfer cassette, such that the genome of the plant cell comprises in the following order the first recombination site, the first nucleotide sequence of interest, the second recombination site, the second nucleotide sequence of interest, and the third recombination site.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的组合物。 提供了在植物或植物细胞的基因组中的精确位置处堆叠多个转移盒的植物和植物细胞。 在具体实施方案中，植物或植物细胞包含至少两个转移盒。 第一转移盒包含侧翼为第一和第二重组位点的第一核苷酸序列，其中第一和第二重组位点不相同。 第二转移盒包含侧翼为第二重组位点的第二核苷酸序列和第三重组位点，其中第三重组位点与第一和所述第二重组位点不相同。 第二重组位点在第一和第二转移盒之间共享，使得植物细胞的基因组按以下顺序包含第一重组位点，目标第一核苷酸序列，第二重组位点，第二重组位点的第二核苷酸序列 兴趣和第三个重组地点。
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25.发明申请COMPOSITIONS AND METHODS TO STACK MULTIPLE NUCLEOTIDE SEQUENCES OF INTEREST IN THE GENOME OF A PLANT 有权在植物基因组中堆叠多个核苷酸序列的组合物和方法公开(公告)号：US20090093059A1
公开(公告)日：2009-04-09
申请号：US12266016
申请日：2008-11-06
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的精确位置堆叠多个核苷酸序列的方法和组合物。 具体而言，将包含不同重组位点的感兴趣的核苷酸序列的转移盒导入包含靶位点的植物中。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。 转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。
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公开(公告)号：US20080320617A1
公开(公告)日：2008-12-25
申请号：US12184564
申请日：2008-08-01
IPC分类号： A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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公开(公告)号：US07148406B2
公开(公告)日：2006-12-12
申请号：US10361460
申请日：2003-02-10
CPC分类号： C12N15/8255 , C07K14/415 , C12N15/52
摘要： The present invention provides methods and compositions relating to altering lignin biosynthesis content and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins which are involved in lignin biosynthesis. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
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公开(公告)号：US06911575B1
公开(公告)日：2005-06-28
申请号：US09579784
申请日：2000-05-26
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N15/10 , C12N15/113 , C12N15/82 , C12N15/87
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译： 提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。 该方法包括将嵌合寡核苷酸引入植物细胞中，其中所述寡核苷酸包含至少两个具有中间DNA残基的RNA残基中间块。 RNA嵌段与植物核苷酸序列同源。 嵌合寡核苷酸能折叠形成双链寡核苷酸。 嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。
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公开(公告)号：US06706951B1
公开(公告)日：2004-03-16
申请号：US09374967
申请日：1999-08-16
申请人： Kanwarpal S. Dhugga , Xun Wang , Benjamin A. Bowen
发明人： Kanwarpal S. Dhugga , Xun Wang , Benjamin A. Bowen
IPC分类号： A01H500
CPC分类号： C12N9/1241 , C12N15/8245 , C12Y207/07022
摘要： The invention relates to the genetic manipulation of plants, particularly to the expression of galactomannan biosynthetic genes in transformed plants. Nucleotide sequences for the GDP-mannose pyrophosphorylase genes and methods for their use are provided. The sequences find use in the production of gum in plants. A nucleic acid encoding a GDP-mannose pyrophosphorylase from maize is taught, as are plants and plant cells transformed with it.
摘要翻译： 本发明涉及植物的遗传操作，特别涉及转化植物中半乳甘露聚糖生物合成基因的表达。 提供了GDP-甘露糖焦磷酸化酶基因的核苷酸序列及其使用方法。 这些序列用于植物中口香糖的生产。一种编码来自玉米的GDP-甘露糖焦磷酸化酶的核酸被教导，以及用它转化的植物和植物细胞。
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30.发明授权Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant 有权在植物基因组中堆叠多个感兴趣的核苷酸序列的组合物和方法公开(公告)号：US06455315B1
公开(公告)日：2002-09-24
申请号：US09438874
申请日：1999-11-12
IPC分类号： C12N1582
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided, Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的精确位置处堆叠多个核苷酸序列的方法和组合物，具体地，包含感兴趣的侧翼为不相同重组位点的核苷酸序列的转移盒用于转化包含靶 现场。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。 转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。
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