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公开(公告)号：US06528700B1
公开(公告)日：2003-03-04
申请号：US09193612
申请日：1998-11-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N1582
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译： 提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。 该方法包括将嵌合寡核苷酸引入具有中间DNA残基的RNA残基中。 RNA嵌段与植物核苷酸序列同源。 嵌合寡核苷酸能折叠形成双链寡核苷酸。 嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。
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公开(公告)号：US06911575B1
公开(公告)日：2005-06-28
申请号：US09579784
申请日：2000-05-26
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N15/10 , C12N15/113 , C12N15/82 , C12N15/87
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译： 提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。 该方法包括将嵌合寡核苷酸引入植物细胞中，其中所述寡核苷酸包含至少两个具有中间DNA残基的RNA残基中间块。 RNA嵌段与植物核苷酸序列同源。 嵌合寡核苷酸能折叠形成双链寡核苷酸。 嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。
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公开(公告)号：US20110030107A1
公开(公告)日：2011-02-03
申请号：US12909944
申请日：2010-10-22
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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4.发明授权Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant 有权在植物基因组中堆叠多个感兴趣的核苷酸序列的组合物和方法公开(公告)号：US07820880B2
公开(公告)日：2010-10-26
申请号：US12266016
申请日：2008-11-06
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的精确位置堆叠多个核苷酸序列的方法和组合物。 具体而言，将包含不同重组位点的感兴趣的核苷酸序列的转移盒导入包含靶位点的植物中。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。 转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。
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公开(公告)号：US06624297B1
公开(公告)日：2003-09-23
申请号：US09455050
申请日：1999-12-06
IPC分类号： C07H2104
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the targeted integration of nucleotide sequences into a plant are provided. Particularly, the present invention is drawn to compositions comprising polynucleotide sequences having the following operably linked components: an intron, a nucleotide sequence of interest, and a terminator region, wherein the polynucleotide sequence comprises one or more recombination sites. The recombination sites are non-identical to one another and one of the recombination sites is contained within the intron.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法和组合物。 特别地，本发明涉及包含具有以下可操作连接的组分的多核苷酸序列的组合物：内含子，目的核苷酸序列和终止区，其中所述多核苷酸序列包含一个或多个重组位点。 重组位点彼此不相同，并且重组位点之一包含在内含子内。
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公开(公告)号：US06175058B1
公开(公告)日：2001-01-16
申请号：US09263128
申请日：1999-03-05
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC分类号： A01H100
CPC分类号： C12N9/00 , C12N15/8213 , C12N15/8289
摘要： A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
摘要翻译： 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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7.发明申请COMPOSITIONS AND METHODS TO STACK MULTIPLE NUCLEOTIDE SEQUENCES OF INTEREST IN THE GENOME OF A PLANT 有权在植物基因组中堆叠多个核苷酸序列的组合物和方法公开(公告)号：US20090093059A1
公开(公告)日：2009-04-09
申请号：US12266016
申请日：2008-11-06
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的精确位置堆叠多个核苷酸序列的方法和组合物。 具体而言，将包含不同重组位点的感兴趣的核苷酸序列的转移盒导入包含靶位点的植物中。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。 转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。
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公开(公告)号：US20080320617A1
公开(公告)日：2008-12-25
申请号：US12184564
申请日：2008-08-01
IPC分类号： A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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9.发明授权Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant 有权在植物基因组中堆叠多个感兴趣的核苷酸序列的组合物和方法公开(公告)号：US06455315B1
公开(公告)日：2002-09-24
申请号：US09438874
申请日：1999-11-12
IPC分类号： C12N1582
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided, Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译： 提供了用于在植物或植物细胞的基因组中的精确位置处堆叠多个核苷酸序列的方法和组合物，具体地，包含感兴趣的侧翼为不相同重组位点的核苷酸序列的转移盒用于转化包含靶 现场。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。 转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。
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公开(公告)号：US08536420B2
公开(公告)日：2013-09-17
申请号：US12184564
申请日：2008-08-01
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译： 提供了将核苷酸序列靶向整合到植物中的方法。 使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。 靶位点至少包含一组与转移盒上相同的非相同重组位点。 通过重组酶交换侧翼为重组位点的核苷酸序列。
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