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    Seed-preferred promoters from end genes
    4.
    发明授权
    Seed-preferred promoters from end genes 有权
    来自末端基因的种子优选启动子

    公开(公告)号：US06528704B1

    公开(公告)日：2003-03-04

    申请号：US09383543

    申请日：1999-08-26

    申请人： Casper Linnestad ,  Kathryn K. Lappegard ,  Shane E. Abbitt ,  Susan J. Martino-Catt ,  Odd-Arne Olsen

    发明人： Casper Linnestad ,  Kathryn K. Lappegard ,  Shane E. Abbitt ,  Susan J. Martino-Catt ,  Odd-Arne Olsen

    IPC分类号： A01H300

    CPC分类号： C12N15/8222 ,  C07K14/415 ,  C12N15/8234

    摘要： The invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are nucleotide sequences for seed-preferred promoters isolated from genes for end1 and end2. A method for expressing a heterologous nucleotide sequence in a plant using the disclosed promoter sequences is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the invention and regenerating a stably transformed plant from the transformed plant cell.

    摘要翻译： 本发明提供用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物是分离自end1和end2基因的种子优选启动子的核苷酸序列。 提供了使用所公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS
    5.
    发明申请
    METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS 审中-公开
    植物核酸序列大规模功能评估方法

    公开(公告)号：US20120329674A1

    公开(公告)日：2012-12-27

    申请号：US13312032

    申请日：2011-12-06

    申请人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    发明人： Marc C. Albertsen ,  Susan J. Martino-Catt ,  Xiaomu Niu ,  Igor Cunha Oliveira ,  Dwight Tomes ,  Haiyin Wang ,  Deping Xu

    IPC分类号： C40B30/06

    CPC分类号： C12N15/1082

    摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

    摘要翻译： 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。

    Seed-preferred promoters
    6.
    发明授权
    Seed-preferred promoters 有权
    种子优选的启动子

    公开(公告)号：US06225529B1

    公开(公告)日：2001-05-01

    申请号：US09377648

    申请日：1999-08-19

    申请人： Kathryn K. Lappegard ,  Susan J. Martino-Catt

    发明人： Kathryn K. Lappegard ,  Susan J. Martino-Catt

    IPC分类号： C12N1509

    CPC分类号： C12N15/8222 ,  C07K14/415 ,  C12N9/90 ,  C12N15/8234

    摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for Cim1 (cytokinin-induced message). cZ19B1 (maize 19 kDa zein); or mi1ps (myo-inositol-1-phosphate synthase). A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the seed-preferred promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

    摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从Cim1基因（细胞分裂素诱导的信号）分离的种子优选启动子的新核苷酸序列。 cZ19B1（玉米19kDa玉米蛋白）; 或mi1ps（肌醇-1-磷酸合酶）。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含可操作地连接到本发明的种子优选启动子之一的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。