公开(公告)号：US08086410B1

公开(公告)日：2011-12-27

申请号：US12117911

申请日：2008-05-09

申请人： Changsheng Liu ,  Fei Gao

发明人： Changsheng Liu ,  Fei Gao

IPC分类号： G06F19/00 ,  C12Q1/68

CPC分类号： G06F19/18 ,  G06F19/22

摘要： A method for detecting DNA variation. First, by aligning trace data of a sample DNA sequence to trace data of a reference DNA sequence to produce an aligned sample DNA sequence. Then, inputting the trace data of the bases of both the reference DNA sequence and the aligned sample DNA sequence for a particular frame number into a non-linear mathematical function of an anti-correlation calculation scheme for all the frame numbers. Minimal values will be produced at the particular frame number for DNA base trace data of the aligned sample DNA sequence which are not a variation as compared to the reference DNA sequence. Values above the minimal values will be produced at the particular frame number for DNA base trace data of the aligned sample DNA sequence which are a variation as compared to the reference DNA sequence.

摘要翻译： 检测DNA变异的方法。 首先，通过将样本DNA序列的痕量数据对齐到追踪参考DNA序列的数据以产生排列的样品DNA序列。 然后，将用于特定帧号的参考DNA序列和对准样品DNA序列的碱基的痕迹数据输入到所有帧号的反相关计算方案的非线性数学函数中。 与对照样品DNA序列的DNA碱基痕量数据的特定帧编号产生最小值，与参考DNA序列相比不是变异。 高于最小值的值将在对准的样品DNA序列的DNA碱基痕量数据的特定帧数产生，这是与参考DNA序列相比的变体。

公开(公告)号：US20090301884A1

公开(公告)日：2009-12-10

申请号：US12154598

申请日：2008-05-23

申请人： Changsheng Liu ,  Hequan Zhao

发明人： Changsheng Liu ,  Hequan Zhao

IPC分类号： C07K1/26

CPC分类号： G06K9/00127 ,  G01N27/44721

摘要： An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

摘要翻译： 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，该系统允许使用不同的染料组来标记在单独泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US07094286B2

公开(公告)日：2006-08-22

申请号：US10468648

申请日：2001-02-26

申请人： Changsheng Liu

发明人： Changsheng Liu

IPC分类号： A61L24/02

CPC分类号： G03G15/09 ,  A61L24/02 ,  A61L2430/02 ,  B82Y15/00 ,  G03G2221/183 ,  Y10S623/923

摘要： The present invention discloses an inorganic bone adhesive and its use in human hard tissue repairs. The inorganic bone adhesive comprises basic compound, phosphate, calcium phosphate bone cement and retarder with the characteristics of rapid hydration rate and high early strength. Inorganic Bone adhesive can be widely used in the artificial joints fixation, screw fixation as well as comminuted fracture fixation. It is a kind of safe and effective adhesive material and beneficial for the fast postoperative recovery. The final hydration reaction products contains the composition of magnesium phosphate, bio-mineral containing ammonium and apatite-like materials, which has excellent biocompatibility and can be gradually absorbed by surrounding tissues after being implanted in vivo, which benefits the in-growth of the new bone.

摘要翻译： 本发明公开了一种无机骨粘合剂及其在人体硬组织修复中的应用。 无机骨粘合剂包括碱性化合物，磷酸盐，磷酸钙骨水泥和缓凝剂，具有水化速度快，早期强度高的特点。 无机骨粘合剂可广泛用于人造关节固定，螺钉固定以及粉碎骨折固定。 它是一种安全有效的粘合剂材料，有利于术后快速恢复。 最终的水化反应产物含有磷酸镁，含有矿物质和磷灰石的物质的生物矿物质组成，具有极好的生物相容性，植物体内可被周围组织逐渐吸收，有利于新生物的生长 骨。