公开(公告)号：US20050161328A1

公开(公告)日：2005-07-28

申请号：US11047355

申请日：2005-01-31

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N21/64 ,  G01N27/447 ,  G01N33/483 ,  G01N33/50 ,  G06K9/00 ,  G01N27/453

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US06863791B1

公开(公告)日：2005-03-08

申请号：US09676526

申请日：2000-10-02

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N21/64 ,  G01N27/447 ,  G01N33/483 ,  G01N33/50 ,  G06K9/00 ,  G01N27/26

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US20110232356A1

公开(公告)日：2011-09-29

申请号：US13053189

申请日：2011-03-21

Applicant: Changsheng LIU ,  Hequan Zhao

Inventor： Changsheng LIU ,  Hequan Zhao

IPC: G01N33/00 ,  G01N27/447

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，该系统允许使用不同的染料组来标记在单独泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US20090301884A1

公开(公告)日：2009-12-10

申请号：US12154598

申请日：2008-05-23

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: C07K1/26

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，该系统允许使用不同的染料组来标记在单独泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US07384525B2

公开(公告)日：2008-06-10

申请号：US11047355

申请日：2005-01-31

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。