公开(公告)号：US07459070B2

公开(公告)日：2008-12-02

申请号：US11204773

申请日：2005-08-15

Applicant: Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

IPC: G01N27/447 ,  G01N27/453

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US07384525B2

公开(公告)日：2008-06-10

申请号：US11047355

申请日：2005-01-31

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US06365024B1

公开(公告)日：2002-04-02

申请号：US09372151

申请日：1999-08-11

Applicant: Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

Inventor： Qingbo Li ,  Thomas E. Kane ,  Changsheng Liu ,  Bernard Sonnenschein ,  Michael V. Sharer ,  John R. Kernan

IPC: G01N2726

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Abstract translation: 公开了一种自动电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有含有凝胶注射器/步进电机或具有泵的高压室的凝胶递送模块，以通过毛细管快速均匀地输送凝胶。 该系统还包括多波长光束发生器，以产生产生具有宽波长范围的光束的激光束。 离线毛细管修复器彻底清洁毛细管柱，以便同时执行另一个毛细管柱的电泳。 离线毛细管再生器的流线型性质提供了增加系统吞吐量的优点，同时最小化了系统成本。

公开(公告)号：US06352633B1

公开(公告)日：2002-03-05

申请号：US09388125

申请日：1999-08-31

Applicant: Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E Kane ,  Qingbo Li

IPC: G01N2726

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresced into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量托盘的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US6118127A

公开(公告)日：2000-09-12

申请号：US449418

申请日：1999-11-29

Applicant: Changsheng Liu ,  Qingbo Li

Inventor： Changsheng Liu ,  Qingbo Li

IPC: G01N21/64 ,  G01N27/447

CPC classification number: G01N27/44721

Abstract: A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS). The TGBS split fluoresced light from a tagged DNA sample into 0th order and a 1st order components, both of which are detected on a two-dimensional detector array of a CCD camera. The 0th and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The TGBS splits fluoresced incoming light into 0th and 1st order components, which are then spread out among a number of pixels in the detector array. The 1st component of this light is received by pixels whose position relative to the 0th order component depends on the frequency of fluorescence. Thus, the position at which signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the 0th order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.

Abstract translation: 用于DNA样品鉴定的检测器具有透射光栅分束器（TGBS）。 TGBS将来自标记的DNA样品的荧光发射到第0阶和第1阶分量，这两个分量在CCD相机的二维检测器阵列上检测。 沿着检测器阵列中的像素列检测第0和第1阶分量，并且彼此间隔开。 DNA样品用四种荧光染料标记，每种核苷酸具有一种染料，所有四种染料以相同的单色激发信号略有不同的方式响应。 TGBS将入射光发射到第0和第1阶分量，然后将其分散在检测器阵列中的多个像素中。 该光的第一分量由相对于第0次分量的位置取决于荧光频率的像素接收。 因此，在阵列上检测到信号能量的位置指示特定染料，因此指示由该染料标记的相应核苷酸。 监测第0级像素和所选第1级像素处的信号能量，提供一组数据，然后可以从中识别特定核苷酸。

公开(公告)号：US20090090630A1

公开(公告)日：2009-04-09

申请号：US12290087

申请日：2008-10-27

Applicant: Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

Inventor： Changsheng Liu ,  Thomas E. Kane ,  Qingbo Li

IPC: G01N27/453

CPC classification number: C07K1/26 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  Y10T436/2575

Abstract: An automated capillary zone electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends is spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a container connected to the detection end of the capillaries. The container is provided with valving which facilitate cleaning the capillaries, loading buffer into the capillaries, introducing samples to be electrophoresed into the capillaries, and performing capillary zone electrophoresis on the thus introduced samples.

Abstract translation: 公开了一种自动化毛细管区域电泳系统。 该系统采用具有多个毛细管的毛细管盒。 盒具有从板的一侧突出的第一阵列的毛细管端。 毛细管端部的第一阵列以与标准尺寸的微量滴定塔的孔基本相同的方式间隔开。 这允许人们同时对存在于托盘的每个孔中的样品进行毛细管电泳。 该系统包括堆叠的双转盘装置，以消除由连续的电泳执行中重复使用相同的缓冲盘引起的交叉污染。 该系统还具有连接到毛细管的检测端的容器。 该容器设置有阀门，其有助于清洁毛细管，将缓冲液加载到毛细管中，将待电泳的样品引入毛细管中，并在这样引入的样品上进行毛细管区带电泳。

公开(公告)号：US20050161328A1

公开(公告)日：2005-07-28

申请号：US11047355

申请日：2005-01-31

Applicant: Changsheng Liu ,  Hequan Zhao

Inventor： Changsheng Liu ,  Hequan Zhao

IPC: G01N21/64 ,  G01N27/447 ,  G01N33/483 ,  G01N33/50 ,  G06K9/00 ,  G01N27/453

CPC classification number: G06K9/00127 ,  G01N27/44721

Abstract: An electrophoretic system having a plurality of separation lanes is provided with an automatic calibration feature in which each lane is separately calibrated. For each lane, the calibration coefficients map a spectrum of received channel intensities onto values reflective of the relative likelihood of each of a plurality of dyes being present. Individual peaks, reflective of the influence of a single dye, are isolated from among the various sets of detected light intensity spectra, and these can be used to both detect the number of dye components present, and also to establish exemplary vectors for the calibration coefficients which may then be clustered and further processed to arrive at a calibration matrix for the system. The system of the present invention thus permits one to use different dye sets to tag DNA nucleotides in samples which migrate in separate lanes, and also allows for in-situ calibration with new, previously unused dye sets.

Abstract translation: 具有多个分离通道的电泳系统设置有自动校准特征，其中每个通道被单独校准。 对于每个通道，校准系数将接收的通道强度的光谱映射到反映存在多种染料中的每一种的相对似然性的值。 反映单一染料的影响的各个峰从各种检测的光强度谱组中分离出来，并且这些峰可用于检测存在的染料成分的数量，并且还可以建立用于校准系数的示例性载体 然后可以将其聚类并进一步处理以得到系统的校准矩阵。 因此，本发明的系统允许使用不同的染料组来标记在分离的泳道中迁移的样品中的DNA核苷酸，并且还允许用新的先前未使用的染料组进行原位校准。

公开(公告)号：US08703208B2

公开(公告)日：2014-04-22

申请号：US12403904

申请日：2009-03-13

Applicant: Changsheng Liu ,  Yuan Yuan

Inventor： Changsheng Liu ,  Yuan Yuan

IPC: A61K33/42 ,  A61P7/04 ,  A61L15/42 ,  C01B37/00

CPC classification number: A61L26/0004 ,  A61L15/18 ,  A61L2400/04 ,  A61L2400/12 ,  C01B33/16 ,  C01B33/163

Abstract: The invention disclosed a novel mesoporous silica-based xerogel and its use in hemorrhage control. The mesoporous silica-based xerogel material has tunable mesopores (1-50 nm), high specific surface area (100-1400 m2/g), macroscopical morphology (powder, film, disc, column, etc.) and adjustable compositions (SiO2, CaO and P2O5, etc.) as well as good biodegradation. The mesoporous silica-based xerogels herein effectively promote the blood clotting under various conditions including slow and severe hemorrhage, even at the blood oozing site of bone defect. Meanwhile, the networks of silica-based xerogel with good elastic and mechanic properties, formed by adsorbing a large amount of water, can modulate the cell behavior and tissue growth, and thus promote the wound healing. Additionally, due to the mesoporous structure, the materials have the potential to load drug, thrombin and bioactive factors, which is favorable for the therapeutical efficacy.

Abstract translation: 本发明公开了一种新型介孔二氧化硅基干凝胶及其在出血控制中的应用。 介孔二氧化硅基干凝胶材料具有可调的介孔（1-50nm），高比表面积（100-1400m 2 / g），宏观形态（粉末，膜，圆盘，柱等）和可调组合物（SiO 2， CaO和P2O5等）以及良好的生物降解性。 本文介孔二氧化硅基干凝胶在各种条件下，即使在骨缺损的血液渗出部位也能有效地促进血液凝固，包括缓慢和严重的出血。 同时，通过吸附大量水形成的具有良好弹性和力学性能的二氧化硅基干凝胶网络可以调节细胞行为和组织生长，从而促进伤口愈合。 另外，由于介孔结构，材料具有加载药物，凝血酶和生物活性因子的潜力，这有利于治疗功效。

公开(公告)号：US20100190209A1

公开(公告)日：2010-07-29

申请号：US12623527

申请日：2009-11-23

Applicant: Changsheng LIU ,  Jun LIN ,  Jiangchao QIAN ,  Yuan YUAN

Inventor： Changsheng LIU ,  Jun LIN ,  Jiangchao QIAN ,  Yuan YUAN

IPC: C12P21/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

CPC classification number: C07K14/51 ,  A61K38/00

Abstract: The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.

Abstract translation: 本发明公开了基于大肠杆菌表达系统的重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列和rhBMP-2的制备方法。 具体而言，本发明提供了适用于大肠杆菌表达系统的最佳DNA序列，rhBMP-2的有效制备方法，以及重组细菌的相关构建，表达和纯化技术。 与未经优化的传统hBMP-2基因相比，大肠杆菌中优化基因的rhBMP-2表达水平提高了50％。 此外，本发明还提供了具有增强的复性效率和纯化产率的长链rhBMP-2的制备方法。

公开(公告)号：US20090232902A1

公开(公告)日：2009-09-17

申请号：US12403904

申请日：2009-03-13

Applicant: Changsheng LIU ,  Yuan YUAN

Inventor： Changsheng LIU ,  Yuan YUAN

IPC: A61K33/42 ,  A61P7/04

CPC classification number: A61L26/0004 ,  A61L15/18 ,  A61L2400/04 ,  A61L2400/12 ,  C01B33/16 ,  C01B33/163

Abstract: The invention disclosed a novel mesoporous silica-based xerogel and its use in hemorrhage control. The mesoporous silica-based xerogel material has tunable mesopores (1-50 nm), high specific surface area (100-1400 m2/g), macroscopical morphology (powder, film, disc, column, etc.) and adjustable compositions (SiO2, CaO and P2O5, etc.) as well as good biodegradation. The mesoporous silica-based xerogels herein effectively promote the blood clotting under various conditions including slow and severe hemorrhage, even at the blood oozing site of bone defect. Meanwhile, the networks of silica-based xerogel with good elastic and mechanic properties, formed by adsorbing a large amount of water, can modulate the cell behavior and tissue growth, and thus promote the wound healing. Additionally, due to the mesoporous structure, the materials have the potential to load drug, thrombin and bioactive factors, which is favorable for the therapeutical efficacy.

Abstract translation: 本发明公开了一种新型介孔二氧化硅基干凝胶及其在出血控制中的应用。 介孔二氧化硅基干凝胶材料具有可调的介孔（1-50nm），高比表面积（100-1400m 2 / g），宏观形态（粉末，膜，圆盘，柱等）和可调组合物（SiO 2， CaO和P2O5等）以及良好的生物降解性。 本文介孔二氧化硅基干凝胶在各种条件下，即使在骨缺损的血液渗出部位也能有效地促进血液凝固，包括缓慢和严重的出血。 同时，通过吸附大量水形成的具有良好弹性和力学性能的二氧化硅基干凝胶网络可以调节细胞行为和组织生长，从而促进伤口愈合。 另外，由于介孔结构，材料具有加载药物，凝血酶和生物活性因子的潜力，这有利于治疗功效。