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公开(公告)号：US08026067B2
公开(公告)日：2011-09-27
申请号：US12724335
申请日：2010-03-15
Applicant: Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Yu-kwan Tong , Chunming Ding
Inventor： Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Yu-kwan Tong , Chunming Ding
CPC classification number: C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2521/331
Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.
Abstract translation: 本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。 更具体地说，本发明依赖于maspin基因在胎儿DNA和母体DNA中差异甲基化的发现，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开，并根据胎儿DNA水平和甲基化异常检测产前疾病 状态。
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22.发明授权Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences 有权用于选择性扩增，检测或定量具有特定末端序列的靶DNA的方法和试剂盒公开(公告)号：US08748100B2
公开(公告)日：2014-06-10
申请号：US11897654
申请日：2007-08-30
Applicant: Yuk Ming Dennis Lo , Yu Kwan Tong , Wai Kwun Rossa Chiu , Chunming Ding
Inventor： Yuk Ming Dennis Lo , Yu Kwan Tong , Wai Kwun Rossa Chiu , Chunming Ding
CPC classification number: C12Q1/686 , C12Q2525/301 , C12Q2521/331
Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.
Abstract translation: 本文公开了用于选择性扩增，检测或定量具有特异末端序列的DNA片段的方法和试剂盒，特别是在限制酶消化后产生。 该方法可用于例如检测低甲基化DNA片段。 该方法和试剂盒特别可用于检测或定量含有相应的高甲基化母体DNA片段的母体血浆样品中的低甲基化胎儿DNA片段。
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公开(公告)号：US08435764B2
公开(公告)日：2013-05-07
申请号：US12773095
申请日：2010-05-04
Applicant: Chunming Ding , Ju Luan , Boping Zhou , Jing Yuan , Xinchun Chen , Hongmei Zhang
Inventor： Chunming Ding , Ju Luan , Boping Zhou , Jing Yuan , Xinchun Chen , Hongmei Zhang
CPC classification number: C12Q1/706 , C12Q2600/156
Abstract: Disclosed is a method for the detection of virus variations. Disclosed also is a method for the treatment of virus infection in a subject based on the detection of virus variations. Additionally, a kit is provided for the detection of virus variations.
Abstract translation: 公开了一种用于检测病毒变异的方法。 还公开了一种基于病毒变异检测来治疗受试者的病毒感染的方法。 另外，提供了一个用于检测病毒变体的工具箱。
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公开(公告)号：US20130084566A1
公开(公告)日：2013-04-04
申请号：US13618527
申请日：2012-09-14
Applicant: YUK MING DENNIS LO , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
Inventor： YUK MING DENNIS LO , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6876 , C12Q1/6883 , C12Q2600/154 , C12Q2600/156
Abstract: This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.
Abstract translation: 该应用描述了在孕妇中发现来自胎儿的某些基因（如RASSF1A，APC，CASP8，RARB，SCGB3A1，DAB2IP，PTPN6，THY1，TMEFF2和PYCARD）高度甲基化，而相同的基因 产妇来源是未甲基化。 该发现允许从孕妇的生物样品中容易地检测这些甲基化胎儿基因中的一种或多种，作为样品中胎儿DNA存在的通用指标。 这些胎儿甲基化标记物作为非侵入性分析过程的阳性对照特别有用，在此过程中监测胎儿DNA的质量和数量。 这些新确定的胎儿标记物也可以直接测量以诊断某些妊娠相关病症。
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公开(公告)号：US20110143342A1
公开(公告)日：2011-06-16
申请号：US12791776
申请日：2010-06-01
Applicant: Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
Inventor： Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6876 , C12Q1/6883 , C12Q2600/154 , C12Q2600/156
Abstract: This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.
Abstract translation: 该应用描述了在孕妇中发现来自胎儿的某些基因（如RASSF1A，APC，CASP8，RARB，SCGB3A1，DAB2IP，PTPN6，THY1，TMEFF2和PYCARD）高度甲基化，而相同的基因 产妇来源是未甲基化。 该发现允许从孕妇的生物样品中容易地检测这些甲基化胎儿基因中的一种或多种，作为样品中胎儿DNA存在的通用指标。 这些胎儿甲基化标记物作为非侵入性分析过程的阳性对照特别有用，在此过程中监测胎儿DNA的质量和数量。 这些新确定的胎儿标记物也可以直接测量以诊断某些妊娠相关病症。
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Patent Agency Ranking
公开(公告)号：US07901884B2
公开(公告)日：2011-03-08
申请号：US11784499
申请日：2007-04-06
Applicant: Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Shengnan Jin , Tracy Yuen Han Lee , Fiona Miu Fun Lun
Inventor： Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Shengnan Jin , Tracy Yuen Han Lee , Fiona Miu Fun Lun
CPC classification number: C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2523/125
Abstract: This application provides the use of novel fetal markers for prenatal diagnosis and monitoring of certain pregnancy-related conditions. More specifically, the invention resides in the discovery that certain CpG islands located on fetal chromosome 21 demonstrate a methylation profile that is distinct from that of the corresponding CpG islands located on maternal chromosome 21. This application also provides kits for diagnosing or monitoring of the relevant conditions.
Abstract translation: 该应用提供了新颖的胎儿标记物用于产前诊断和监测某些妊娠相关病症的用途。 更具体地，本发明在于发现位于胎儿染色体21上的某些CpG岛表现出与位于母体染色体21上的相应CpG岛不同的甲基化谱。该应用还提供用于诊断或监测相关 条件。
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公开(公告)号：US07754428B2
公开(公告)日：2010-07-13
申请号：US11784501
申请日：2007-04-06
Applicant: Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
Inventor： Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Chunming Ding , Kwan Chee Chan , Hing Nam Ivy Wong , Ka Chun Ryan Yuen
CPC classification number: C12Q1/68 , C12Q1/6876 , C12Q1/6883 , C12Q2600/154 , C12Q2600/156
Abstract: This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.
Abstract translation: 该应用描述了在孕妇中发现来自胎儿的某些基因（如RASSF1A，APC，CASP8，RARB，SCGB3A1，DAB2IP，PTPN6，THY1，TMEFF2和PYCARD）高度甲基化，而相同的基因 产妇来源是未甲基化。 该发现允许从孕妇的生物样品中容易地检测这些甲基化胎儿基因中的一种或多种，作为样品中胎儿DNA存在的通用指标。 这些胎儿甲基化标记物作为非侵入性分析过程的阳性对照特别有用，在此过程中监测胎儿DNA的质量和数量。 这些新确定的胎儿标记物也可以直接测量以诊断某些妊娠相关病症。
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公开(公告)号：US07709194B2
公开(公告)日：2010-05-04
申请号：US11144951
申请日：2005-06-03
Applicant: Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Yu-kwan Tong , Chunming Ding
Inventor： Yuk Ming Dennis Lo , Rossa Wai Kwun Chiu , Stephen Siu Chung Chim , Yu-kwan Tong , Chunming Ding
CPC classification number: C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2600/156 , C12Q2521/331
Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.
Abstract translation: 本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。 更具体地说，本发明依赖于maspin基因在胎儿DNA和母体DNA中差异甲基化的发现，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开，并根据胎儿DNA水平和甲基化异常检测产前疾病 状态。
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29.发明申请Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences 有权用于选择性扩增，检测或定量具有特定末端序列的靶DNA的方法和试剂盒公开(公告)号：US20090061425A1
公开(公告)日：2009-03-05
申请号：US11897654
申请日：2007-08-30
Applicant: Yuk Ming Dennis Lo , Yu Kwan Tong , Wai Kwun Rossa Chiu , Chunming Ding
Inventor： Yuk Ming Dennis Lo , Yu Kwan Tong , Wai Kwun Rossa Chiu , Chunming Ding
CPC classification number: C12Q1/686 , C12Q2525/301 , C12Q2521/331
Abstract: Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment.
Abstract translation: 本文公开了用于选择性扩增，检测或定量具有特异末端序列的DNA片段的方法和试剂盒，特别是在限制酶消化后产生。 该方法可用于例如检测低甲基化DNA片段。 该方法和试剂盒特别可用于检测或定量含有相应的高甲基化母体DNA片段的母体血浆样品中的低甲基化胎儿DNA片段。
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公开(公告)号：US20070207466A1
公开(公告)日：2007-09-06
申请号：US11364294
申请日：2006-02-28
Applicant: Charles Cantor , Chunming Ding , Yuk Lo , Rossa Chiu
Inventor： Charles Cantor , Chunming Ding , Yuk Lo , Rossa Chiu
IPC: C12Q1/68
CPC classification number: C12Q1/6881 , C12Q1/6872 , C12Q2600/156 , C12Q2600/172 , C12Q2535/125
Abstract: The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample.
Abstract translation: 本发明涉及检测生物样品中核酸的方法。 该方法基于提供优异分析特异性的碱基延伸反应和提供优异特异性的质谱分析的新型组合。 该方法可用于例如诊断，预后和治疗目的。 该方法允许精确检测在生物样品中以非常少的量存在的核酸。 例如，本发明的方法优选用于检测母体血液样品中的胎儿核酸; 在血液，尿液或粪便样品中循环肿瘤特异性核酸; 和供体特异性核酸。 在另一个实施方案中，可以检测生物样品中的病毒，细菌，真菌或其它外来核酸。
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