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公开(公告)号：US20120165393A1
公开(公告)日：2012-06-28
申请号：US13326433
申请日：2011-12-15
申请人： David B. Rozema , Darren H. Wakefield , David L. Lewis , Jon A. Wolff , Andrei V. Blokhin , Jonathan D. Benson , Jeffrey C. Carlson , Philipp Hadwiger , Eric A. Kitas , Torsten Hoffman , Kerstin Jahn-Hofmann , Peter Mohr , Hans Martin Mueller , Guenther Ott , Ingo Roehl
发明人： David B. Rozema , Darren H. Wakefield , David L. Lewis , Jon A. Wolff , Andrei V. Blokhin , Jonathan D. Benson , Jeffrey C. Carlson , Philipp Hadwiger , Eric A. Kitas , Torsten Hoffman , Kerstin Jahn-Hofmann , Peter Mohr , Hans Martin Mueller , Guenther Ott , Ingo Roehl
CPC分类号： A61K47/48261 , A61K47/549 , A61K47/64 , A61K47/6415 , C12N15/111 , C12N2310/14 , C12N2310/351 , C12N2310/3515 , C12N2320/32
摘要： The present invention is directed compositions for targeted delivery of RNA interference (RNAi) polynucleotides to hepatocytes in vivo. Targeted RNAi polynucleotides are administered together with co-targeted melittin delivery peptides. Delivery peptides provide membrane penetration function for movement of the RNAi polynucleotides from outside the cell to inside the cell. Reversible modification provides physiological responsiveness to the delivery peptides.
摘要翻译： 本发明涉及用于将体内RNA干扰（RNAi）多核苷酸靶向递送至肝细胞的组合物。 靶向的RNAi多核苷酸与共靶向的蜂毒肽递送肽一起施用。 递送肽提供膜穿透功能，用于将RNAi多核苷酸从细胞外部移动到细胞内。 可逆修饰提供对递送肽的生理反应性。
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公开(公告)号：US07148205B2
公开(公告)日：2006-12-12
申请号：US10600290
申请日：2003-06-20
申请人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号： C12N15/87 , A61K31/70 , A61K47/59 , A61K47/593 , A61K47/645 , A61K48/00 , A61K48/0083 , C07H21/00 , C12N15/111 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/3233 , C12N2320/32 , A61K2300/00
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
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公开(公告)号：US06379966B2
公开(公告)日：2002-04-30
申请号：US09450315
申请日：1999-11-29
申请人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
IPC分类号： C12N1585
CPC分类号： A61K48/0041 , A61K48/0008 , A61K48/0083
摘要： Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
摘要翻译： 公开了用于在细胞中提供核酸表达的复合物。 将多核苷酸和聚合物混合在一起形成复合物，其中络合物的ζ电位不是正的。 然后将复合物递送到其中表达核酸的细胞。
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公开(公告)号：US06867196B1
公开(公告)日：2005-03-15
申请号：US09330909
申请日：1999-06-11
申请人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号： A61K48/0008 , A61K48/0075
摘要： A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
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公开(公告)号：US06627616B2
公开(公告)日：2003-09-30
申请号：US09447966
申请日：1999-11-23
申请人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
IPC分类号： C12N1500
CPC分类号： C12N15/113 , A61K31/70 , A61K47/58 , A61K47/59 , A61K47/593 , A61K47/62 , A61K47/6455 , A61K48/00 , A61K48/0008 , A61K48/0041 , A61K48/0083 , C07H21/00 , C12N15/87 , C12N2320/32 , A61K2300/00
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
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26.发明授权公开(公告)号：US07208314B2
公开(公告)日：2007-04-24
申请号：US10083456
申请日：2002-02-26
IPC分类号： C12N5/00
CPC分类号： A61K48/0025 , A61K48/0008 , A61K48/0041 , A61K48/0075 , A61K48/0083
摘要： A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
摘要翻译： 关于使用pH敏感递送系统将期望的化合物（例如药物和核酸）递送到细胞中的系统。 该系统提供用于将化合物递送和释放到细胞中的组合物和方法。
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公开(公告)号：US07098032B2
公开(公告)日：2006-08-29
申请号：US11046590
申请日：2005-01-28
申请人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
发明人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/60 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07098030B2
公开(公告)日：2006-08-29
申请号：US10899509
申请日：2004-07-26
申请人： David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
发明人： David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07033607B2
公开(公告)日：2006-04-25
申请号：US10095789
申请日：2002-03-11
申请人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
发明人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
IPC分类号： A61K9/127 , A61K31/713
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07019113B2
公开(公告)日：2006-03-28
申请号：US10444662
申请日：2003-05-23
IPC分类号： C07K1/00
CPC分类号： C12N15/87 , A61K38/00 , A61K47/60 , A61K48/00 , A61K48/0008 , A61K48/0041 , C07H21/00 , C07H21/04 , C07K7/06
摘要： An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
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