公开(公告)号：US6053856A

公开(公告)日：2000-04-25

申请号：US853374

申请日：1997-05-08

申请人： Dennis Hlavinka

发明人： Dennis Hlavinka

IPC分类号： A61M1/02 ,  A61M1/36 ,  B01D21/26 ,  B04B5/00 ,  B04B5/04 ,  B04B11/04 ,  B04B7/08

CPC分类号： A61M1/3693 ,  A61M1/0227 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3696 ,  B04B5/0428 ,  B04B5/0442 ,  A61M1/0209 ,  B04B2005/0471

摘要： An apparatus and method are provided for separating components of a fluid or particles. A separation vessel having a barrier dam is provided to initially separate an intermediate density components of a fluid, and a fluid chamber is provided to further separate these the intermediate density component by forming an elutriative field or saturated fluidized particle bed. The separation vessel is placed in a retainer on a centrifuge rotor and ridges in the retainer form various dams in the separation vessel. Also discloses are tubing sets and various separation vessels for use with the apparatus.

摘要翻译： 提供了用于分离流体或颗粒的组分的装置和方法。 提供具有阻挡坝的分离容器以最初分离流体的中间密度分量，并且提供流体室以通过形成淘析场或饱和流化颗粒床来进一步分离这些中间密度组分。 分离容器放置在离心机转子上的保持器中，并且保持器中的脊形成分离容器中的各种坝。 还公开了用于该装置的管组和各种分离容器。

公开(公告)号：US5913768A

公开(公告)日：1999-06-22

申请号：US676039

申请日：1996-07-05

申请人： Robert Langley ,  Dennis Hlavinka ,  Linda A. Taylor ,  John C. Walker

发明人： Robert Langley ,  Dennis Hlavinka ,  Linda A. Taylor ,  John C. Walker

IPC分类号： A61M1/02 ,  A61M1/36 ,  B04B5/04 ,  B04B1/00 ,  B04B11/02

CPC分类号： A61M1/3693 ,  A61M1/0227 ,  A61M1/0236 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3696 ,  B04B5/0442 ,  B04B2005/045 ,  B04B2005/0471

摘要： An apparatus and method are disclosed for filtering or separating particles. The apparatus has a motor coupled to a centrifuge rotor rotatable about an axis of rotation. A fluid chamber rotates with the rotor. A substance is supplied to the inlet of the chamber. At least one of the motor and the substance supply are controlled to maintain a saturated fluidized bed of first particles within the fluid chamber while causing second particles to be retained in the chamber.

公开(公告)号：US5792038A

公开(公告)日：1998-08-11

申请号：US648503

申请日：1996-05-15

申请人： Dennis Hlavinka

发明人： Dennis Hlavinka

IPC分类号： B04B5/04 ,  B04B7/08

CPC分类号： B04B5/0442 ,  B04B2005/045 ,  B04B2005/0471

摘要： A centrifuge separation device is disclosed and includes a rotor configured to be connected to a centrifuge motor for rotation about an axis of rotation. A retainer is associated with the rotor and defines a passageway for a separation channel. A protrusion formed in one of the passageway walls extends towards and is spaced from the other of the passageway walls. The protrusion is sized to substantially block passage of materials in a predetermined density range and to substantially permit passage of materials outside of the predetermined density range. An indentation formed adjacent the protrusion in a wall of the passageway opposite the protrusion is configured to trap fluid during rotation of the rotor and to cooperate with the trapped fluid to maintain a substantially Coriolis-free pathway in a region of the passageway adjacent the protrusion.

摘要翻译： 公开了一种离心机分离装置，其包括转子，该转子被配置为连接到离心机马达上以围绕旋转轴线旋转。 保持器与转子相关联并且限定用于分离通道的通道。 形成在其中一个通道壁中的突起朝向并且与另一个通道壁间隔开。 突起的尺寸被设计成基本上阻挡材料在预定的密度范围内的通过并且基本允许材料在预定的密度范围之外的通过。 在与突起相对的通道的壁中邻近突起形成的凹陷构造成在转子旋转期间捕获流体并与捕获的流体配合以在靠近突起的通道的区域中保持基本上无科里奥利的通道。

公开(公告)号：US5722926A

公开(公告)日：1998-03-03

申请号：US672089

申请日：1996-06-27

申请人： Dennis Hlavinka ,  Robert Langley ,  Linda Taylor ,  John C. Walker

发明人： Dennis Hlavinka ,  Robert Langley ,  Linda Taylor ,  John C. Walker

IPC分类号： A61M1/02 ,  A61M1/36 ,  B04B5/04 ,  B01D21/26

CPC分类号： B04B5/0442 ,  A61M1/0227 ,  A61M1/0236 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3693 ,  A61M1/3696 ,  B04B2005/0471

摘要： A fluid chamber and method are disclosed for filtering or separating particles. The fluid chamber includes a wall extending between an inlet and outlet to form a fluid chamber interior. The interior converges from a position of maximum cross-sectional area toward the inlet. A groove or a step may be formed on an inner surface of the fluid chamber wall to improve particle separation within the fluid chamber.

摘要翻译： 公开了用于过滤或分离颗粒的流体室和方法。 流体室包括在入口和出口之间延伸以形成流体室内部的壁。 内部从最大横截面积的位置朝向入口收敛。 可以在流体室壁的内表面上形成凹槽或台阶以改善流体室内的颗粒分离。

公开(公告)号：US5704889A

公开(公告)日：1998-01-06

申请号：US422598

申请日：1995-04-14

申请人： Dennis Hlavinka ,  Thomas J. Felt

发明人： Dennis Hlavinka ,  Thomas J. Felt

IPC分类号： A61K35/14 ,  B04B5/04 ,  B04B11/04

CPC分类号： B04B5/0442 ,  B04B2005/045

摘要： A centrifuge apparatus is used for collecting white blood cells (WBC), primarily mononuclear cells, from whole blood stratified into layers. A thin mononuclear (MNC) layer is formed at the interface of red blood cells and plasma. A barrier is positioned in the separation vessel of the centrifuge at a location to intercept the thin layer. MNC fluid is allowed to pool behind the barrier before collection is started. To collect the MNC pool, the stratified red blood cell layer is raised from below the interface level by slowing or reversing flow in the RBC exit line thereby causing the MNC pool to spill over the barrier into a well in which a collect line is positioned. Collection ceases when a desired percentage of the pool is removed and the normal position of the interface is re-established; thereafter the pool builds again. By raising the MNC pool from below, improvements in purity and collect volume are achieved. The collection procedure can be useful for harvesting granulocytes and, in general, any sparse stratified component of a centrifuged solution where the sparse component is layered between more dense and less dense strata.

摘要翻译： 离心机用于从分层成层的全血中收集白细胞（WBC），主要是单核细胞。 在红细胞和血浆的界面处形成薄单核（MNC）层。 隔离物位于离心机的分离容器中，以拦截薄层。 允许MNC流体在收集开始之前集中在障碍物后面。 为了收集MNC池，分层的红细胞层通过减缓或逆转RBC出口线中的流动而从界面水平以下升高，从而导致MNC池溢出障碍物进入收集线所在的井中。 当池的所需百分比被移除并且接口的正常位置被重新建立时，收集停止; 此后池再次建成。 通过从下方提高跨国公司池，实现了纯度和收集量的提高。 采集程序可用于收获粒细胞和一般来说，离心溶液的任何稀疏分层组分，其中稀疏组分分层在更致密和较不密集的层之间。