公开(公告)号：US20220403353A1

公开(公告)日：2022-12-22

申请号：US17705043

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20220403352A1

公开(公告)日：2022-12-22

申请号：US17705020

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US11426732B2

公开(公告)日：2022-08-30

申请号：US17082445

申请日：2020-10-28

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Leon Zilun Zhang ,  Chunhong Zhou ,  Matthew Kellinger ,  Michael Previte

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US11236388B1

公开(公告)日：2022-02-01

申请号：US17377285

申请日：2021-07-15

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte

IPC: C12Q1/6874

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20210269793A1

公开(公告)日：2021-09-02

申请号：US17320042

申请日：2021-05-13

Applicant: Element Biosciences, Inc.

Inventor： Matthew Kellinger ,  Sinan ARSLAN ,  Michael PREVITE ,  Junhua Zhao

IPC: C12N15/10 ,  C12Q1/6869 ,  C12N9/12

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing.

公开(公告)号：US20200370113A1

公开(公告)日：2020-11-26

申请号：US16895999

申请日：2020-06-08

Applicant: Element Biosciences, Inc.

Inventor： Matthew Kellinger ,  Michael Previte ,  Molly He ,  Tyler Lopez

IPC: C12Q1/6869

Abstract: Compositions and methods comprising the use of polymerase-nucleotide conjugates for nucleic acid sequencing and analysis applications are described.

公开(公告)号：US10704094B1

公开(公告)日：2020-07-07

申请号：US16740357

申请日：2020-01-10

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan Arslan ,  Chunhong Zhou ,  Molly Min He ,  Matthew Kellinger ,  Adeline Huizhen Mah ,  Michael Previte ,  Lei Sun

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/543 ,  G01N21/64 ,  C12N9/12

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.