公开(公告)号：US12270056B2

公开(公告)日：2025-04-08

申请号：US17705020

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12139727B2

公开(公告)日：2024-11-12

申请号：US17705043

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US11788075B2

公开(公告)日：2023-10-17

申请号：US18160947

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik Hentschel ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger ,  Mark Ambroso

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12091657B2

公开(公告)日：2024-09-17

申请号：US17120030

申请日：2020-12-11

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Matthew Kellinger ,  Molly He ,  Tyler Lopez

IPC: C12N15/10 ,  C12N9/12 ,  C12P21/00 ,  C12Q1/6874

CPC classification number: C12N15/102 ,  C12N9/1276 ,  C12N15/1068 ,  C12Q1/6874 ,  C12Y207/07049 ,  C12N2310/321 ,  C12P21/00 ,  C12Q1/6874 ,  C12Q2521/107 ,  C12Q2525/186

Abstract: Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.

公开(公告)号：US11427855B1

公开(公告)日：2022-08-30

申请号：US17377283

申请日：2021-07-15

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6834 ,  G01N21/64 ,  C12Q1/6874

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US11198121B1

公开(公告)日：2021-12-14

申请号：US17218027

申请日：2021-03-30

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Leon Zilun Zhang ,  Chunhong Zhou ,  Matthew Kellinger ,  Michael Previte ,  Sinan Arslan ,  Molly He ,  Hui Zhen Mah ,  Lei Sun

IPC: G01N21/64 ,  B01L3/00 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US10768173B1

公开(公告)日：2020-09-08

申请号：US16579794

申请日：2019-09-23

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly He ,  Matthew Kellinger ,  Jake Levieux ,  Michael Previte ,  Junhua Zhao ,  Su Zhang

IPC: C12Q1/6869 ,  C07H21/00 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6874

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US12241891B2

公开(公告)日：2025-03-04

申请号：US17675154

申请日：2022-02-18

Applicant: Element Biosciences, Inc.

Inventor： Michael Previte ,  Molly He ,  Junhua Zhao ,  Hui Zhen Mah ,  Chunhong Zhou ,  Sinan Arslan ,  Matthew Kellinger ,  Lorenzo Berti ,  Steve Xiangling Chen

IPC: G01N33/53 ,  C12Q1/68 ,  C12Q1/6874 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US12163163B2

公开(公告)日：2024-12-10

申请号：US18160952

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik Boothby-Hentschel ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger ,  Mark Ambroso

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12134766B2

公开(公告)日：2024-11-05

申请号：US18153268

申请日：2023-01-11

Applicant: Element Biosciences, Inc.

Inventor： Matthew Kellinger ,  Sinan Arslan ,  Michael Previte ,  Junhua Zhao

IPC: C12N15/10 ,  C12N9/12 ,  C12Q1/6869

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing.