公开(公告)号：US11408032B2

公开(公告)日：2022-08-09

申请号：US17016350

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: G01N21/64 ,  C12Q1/6869 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.

公开(公告)号：US11365444B2

公开(公告)日：2022-06-21

申请号：US17372361

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11339433B2

公开(公告)日：2022-05-24

申请号：US17496710

申请日：2021-10-07

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N15/14 ,  G01N21/64 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

公开(公告)号：US11261489B2

公开(公告)日：2022-03-01

申请号：US17372359

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11198121B1

公开(公告)日：2021-12-14

申请号：US17218027

申请日：2021-03-30

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Leon Zilun Zhang ,  Chunhong Zhou ,  Matthew Kellinger ,  Michael Previte ,  Sinan Arslan ,  Molly He ,  Hui Zhen Mah ,  Lei Sun

IPC: G01N21/64 ,  B01L3/00 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US12241891B2

公开(公告)日：2025-03-04

申请号：US17675154

申请日：2022-02-18

Applicant: Element Biosciences, Inc.

Inventor： Michael Previte ,  Molly He ,  Junhua Zhao ,  Hui Zhen Mah ,  Chunhong Zhou ,  Sinan Arslan ,  Matthew Kellinger ,  Lorenzo Berti ,  Steve Xiangling Chen

IPC: G01N33/53 ,  C12Q1/68 ,  C12Q1/6874 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US11287422B2

公开(公告)日：2022-03-29

申请号：US17356929

申请日：2021-06-24

Applicant: Element Biosciences, Inc.

Inventor： Michael Previte ,  Molly Min He ,  Junhua Zhao ,  Hui Zhen Mah ,  Chunhong Zhou ,  Sinan Arslan ,  Matthew Kellinger ,  Lorenzo Berti ,  Steve Xiangling Chen

IPC: G01N33/53 ,  G01N33/58 ,  C12Q1/68 ,  C12Q1/6874

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210223531A1

公开(公告)日：2021-07-22

申请号：US17151020

申请日：2021-01-15

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Michael Previte ,  Steven Xiangling Chen ,  Chunhong Zhou

IPC: G02B21/16 ,  B01L3/00 ,  G01N21/64 ,  G02B21/06 ,  G02B21/02

Abstract: Optical systems for DNA sequencing and other assays are described. Microscope designs may include a light source configured to emit an excitation beam and an objective lens disposed to receive the excitation beam, direct the excitation beam to a specimen, and receive emission light emitted by the specimen in response to the excitation beam. A plurality of detection channels includes optics configured to receive at least a portion of the emission light. A first dichroic filter can be disposed to reflect the excitation beam into the objective lens and to transmit the emission light, and a second dichroic filter can be disposed to receive the transmitted emission light, transmit a first portion of the transmitted emission light to a first channel of the plurality of channels, and reflect a second portion of the transmitted emission light to a second channel of the plurality of channels. Imaging or detection performance may further be improved by a reduced angle of incidence between the emission light and some or all of the dichroic filters, and/or by linearly polarizing the excitation beam such that the excitation beam is s-polarized with respect to the first dichroic filter.

公开(公告)号：US20210223530A1

公开(公告)日：2021-07-22

申请号：US16902188

申请日：2020-06-15

Applicant: Element Biosciences, Inc.

Inventor： Minghao Guo ,  Michael Previte ,  Steven Xiangling Chen ,  Chunhong Zhou

IPC: G02B21/16 ,  G02B21/02 ,  G02B21/36 ,  G02B21/06 ,  G01N21/64

Abstract: Multi-channel fluorescence microscopes and optical systems may include a light source configured to emit an excitation beam and an objective lens disposed to receive the excitation beam, direct the excitation beam to a specimen, and receive emission light emitted by the specimen in response to the excitation beam. A plurality of detection channels include optics configured to receive at least a portion of the emission light. A first dichroic filter can be disposed to reflect the excitation beam into the objective lens and to transmit the emission light, and a second dichroic filter can be disposed to receive the transmitted emission light, transmit a first portion of the transmitted emission light to a first channel of the plurality of channels, and reflect a second portion of the transmitted emission light to a second channel of the plurality of channels.

公开(公告)号：US11060138B1

公开(公告)日：2021-07-13

申请号：US17016353

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N15/14

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.