公开(公告)号：US20160053296A1

公开(公告)日：2016-02-25

申请号：US14462594

申请日：2014-08-19

Applicant: General Electric Company

Inventor： Chengkun Zhang ,  Kenneth Roger Conway ,  Donald Joseph Buckley ,  Eugene Pauling Boden ,  Weston Blaine Griffin ,  Anshika Bajaj ,  Reginald Donovan Smith ,  Zhipeng Zhang

IPC: C12Q1/24 ,  B01L3/00

CPC classification number: C12M23/40 ,  B01L3/561 ,  B01L2200/141 ,  C12M33/06 ,  C12M37/02 ,  G01N1/18

Abstract: A sampling assembly configured to be coupled to a sample source is provided. The sampling assembly is configured to facilitate aseptic sampling at one or more instances in time. The sampling assembly includes a first conduit having a first port and a second port, where the first port is configured to be coupled to the sample source, and where the second port is configured to be hermetically sealed. The sampling assembly further includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connection points, and where each of the sub-ports is in fluidic communication with the first conduit. Moreover, the sampling assembly includes a plurality of sampling kits, where each sampling kit of the plurality of sampling kits is operatively connected to a respective sub-port of a corresponding sub-conduit.

Abstract translation: 提供了一种被配置为耦合到采样源的采样组件。 采样组件被配置为便于在一个或多个实例的时间进行无菌取样。 采样组件包括具有第一端口和第二端口的第一导管，其中第一端口被配置为耦合到采样源，并且其中第二端口被配置为气密密封。 采样组件还包括具有对应子端口的多个子导管，其中多个子导管中的每一个在相应的连接点处可操作地耦合到第一导管，并且其中每个子端口处于流体连通 与第一个导管。 此外，采样组件包括多个采样套件，其中多个采样套件的每个采样套件可操作地连接到相应副导管的相应子端口。

公开(公告)号：US20140186946A1

公开(公告)日：2014-07-03

申请号：US13839049

申请日：2013-03-15

Applicant: General Electric Company

Inventor： Brian Michael Davis ,  Evelina Roxana Loghin ,  Kenneth Roger Conway ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne

IPC: C12N5/0735

CPC classification number: C12N5/0068 ,  B29C37/0025 ,  B29C59/02 ,  B29C71/0081 ,  B29K2025/06 ,  B29K2105/256 ,  B29K2883/00 ,  B29L2031/712 ,  C12N2501/999 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  C12N2539/10 ,  Y10T156/1039

Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。