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公开(公告)号：US07432048B2
公开(公告)日：2008-10-07
申请号：US09732622
申请日：2000-12-08
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US07303869B2
公开(公告)日：2007-12-04
申请号：US10269222
申请日：2002-10-11
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
CPC分类号： C12Q1/683 , C12Q2561/109 , C12Q2565/101
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07485443B2
公开(公告)日：2009-02-03
申请号：US10197189
申请日：2002-07-17
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
IPC分类号： C12P19/34
CPC分类号： C12Q1/683 , C12Q2565/549 , C12Q2561/109
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07820387B2
公开(公告)日：2010-10-26
申请号：US11926120
申请日：2007-10-28
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07527928B2
公开(公告)日：2009-05-05
申请号：US10309584
申请日：2002-12-04
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/683
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06692917B2
公开(公告)日：2004-02-17
申请号：US09940244
申请日：2001-08-27
申请人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
发明人： Bruce P. Neri , Jeff G. Hall , Victor Lyamichev , Lloyd M. Smith
IPC分类号： C12Q168
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在树枝状大分子上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20080131890A1
公开(公告)日：2008-06-05
申请号：US11809567
申请日：2007-06-01
申请人： Hatim T. Allawi , Victor Lyamichev
发明人： Hatim T. Allawi , Victor Lyamichev
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C07H21/02 , C12Q1/68 , C12Q2525/161 , C12Q2521/107 , C12Q2561/109
摘要： The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
摘要翻译： 本发明涉及用于检测和表征小核酸分子（例如RNA（例如，小RNA如微RNA（miRNA）和小干扰RNA（siRNA））和其它短核酸分子的组合物和方法。 更具体地说，本发明涉及检测和定量RNA表达的方法。 本发明进一步提供了miRNA和siRNA变体的检测。
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公开(公告)号：US20120231461A1
公开(公告)日：2012-09-13
申请号：US13479634
申请日：2012-05-24
申请人： Hatim T. Allawi , Victor Lyamichev
发明人： Hatim T. Allawi , Victor Lyamichev
CPC分类号： C12Q1/6816 , C07H21/02 , C12Q1/68 , C12Q2525/161 , C12Q2521/107 , C12Q2561/109
摘要： The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
摘要翻译： 本发明涉及用于检测和表征小核酸分子（例如RNA（例如，小RNA如微RNA（miRNA）和小干扰RNA（siRNA））和其它短核酸分子的组合物和方法。 更具体地说，本发明涉及检测和定量RNA表达的方法。 本发明进一步提供了miRNA和siRNA变体的检测。
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公开(公告)号：US08206904B2
公开(公告)日：2012-06-26
申请号：US11809567
申请日：2007-06-01
申请人： Hatim T. Allawi , Victor Lyamichev
发明人： Hatim T. Allawi , Victor Lyamichev
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C07H21/02 , C12Q1/68 , C12Q2525/161 , C12Q2521/107 , C12Q2561/109
摘要： The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants.
摘要翻译： 本发明涉及用于检测和表征小核酸分子（例如RNA（例如，小RNA如微RNA（miRNA）和小干扰RNA（siRNA））和其它短核酸分子的组合物和方法。 更具体地说，本发明涉及检测和定量RNA表达的方法。 本发明进一步提供了miRNA和siRNA变体的检测。
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公开(公告)号：US08190371B2
公开(公告)日：2012-05-29
申请号：US12206436
申请日：2008-09-08
申请人： Hatim T. Allawi , Victor Lyamichev , Rex Piper
发明人： Hatim T. Allawi , Victor Lyamichev , Rex Piper
CPC分类号： C12Q1/6851 , Y10T436/10 , C12Q2537/165
摘要： The present invention provides methods and software applications for quantifying a target in an experimental sample by collecting and processing initial signal data from the experimental sample and at least two standard control samples containing known target copy numbers. In this regards, the present invention allows the quantification of target copy number in the experimental sample.
摘要翻译： 本发明提供了通过收集和处理来自实验样品的初始信号数据和至少两个包含已知目标拷贝数的标准对照样品来量化实验样品中靶标的方法和软件应用。 在这方面，本发明允许定量实验样品中的目标拷贝数。
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