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公开(公告)号：US07303869B2
公开(公告)日：2007-12-04
申请号：US10269222
申请日：2002-10-11
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
CPC分类号： C12Q1/683 , C12Q2561/109 , C12Q2565/101
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07485443B2
公开(公告)日：2009-02-03
申请号：US10197189
申请日：2002-07-17
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
IPC分类号： C12P19/34
CPC分类号： C12Q1/683 , C12Q2565/549 , C12Q2561/109
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07256020B2
公开(公告)日：2007-08-14
申请号：US10356861
申请日：2003-02-03
申请人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew Lukowiak
发明人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew Lukowiak
CPC分类号： C12Q1/6869 , A61K38/00 , C07K2319/02 , C12N9/1252 , C12N9/22 , C12N2710/16111 , C12Q1/6844 , C12Q1/6865 , C12Q2525/307 , C12Q2521/301 , C12Q2521/337
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在目标序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07195871B2
公开(公告)日：2007-03-27
申请号：US10290386
申请日：2002-11-07
申请人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
发明人： Victor Lyamichev , Bruce P. Neri , Jeff Hall , Andrew A. Lukowiak
CPC分类号： C12Q1/6827 , A61K38/00 , C07K2319/02 , C12N9/1252 , C12N9/22 , C12Q2561/109 , C12Q2525/125 , C12Q2525/179 , C12Q2537/143
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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5.发明申请Single nucleotide polymorphism analysis using surface invasive cleavage reactions 审中-公开使用表面侵入性切割反应的单核苷酸多态性分析公开(公告)号：US20050003355A1
公开(公告)日：2005-01-06
申请号：US10420247
申请日：2003-04-22
申请人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
发明人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
IPC分类号： C12Q1/68
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6827 , C12Q1/6837 , C12Q2565/501 , C12Q2561/109 , C12Q2565/101 , C12Q2521/319 , C12Q2565/543 , C12Q2537/143
摘要： Disclosed are a method and a corresponding composition of matter for detecting single nucleotide polymorphisms. The composition of matter includes a metal substrate, a probe oligonucleotide immobilized on the substrate; and an upstream oligonucleotide either immobilized on the substrate or in a solution in contact with the substrate. The probe oligonucleotide and the upstream oligonucleotide participate cooperatively in an invasive cleavage reaction when the substrate is contacted with a cleavage agent and a target nucleic acid. Under proper reactions conditions, the probe oligonucleotide, the upstream oligonucleotide, the target nucleotide, and the cleavage agent cooperative result in the formation of a cleavage product at or near the location of a single nucleotide polymorphism of interest.
摘要翻译： 公开了用于检测单核苷酸多态性的方法和相应的物质组成。 物质组成包括金属底物，固定在基底上的探针寡核苷酸; 以及固定在基底上或与基底接触的溶液中的上游寡核苷酸。 当底物与切割剂和靶核酸接触时，探针寡核苷酸和上游寡核苷酸协同参与侵入性切割反应。 在适当的反应条件下，探针寡核苷酸，上游寡核苷酸，靶核苷酸和切割剂协同导致在感兴趣的单核苷酸多态性位置处或附近形成切割产物。
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公开(公告)号：US20060147955A1
公开(公告)日：2006-07-06
申请号：US11266723
申请日：2005-11-03
申请人： Hatim Allawi , Victor Lyamichev , Vecheslav Elagin , Scott Law , Jeff Hall
发明人： Hatim Allawi , Victor Lyamichev , Vecheslav Elagin , Scott Law , Jeff Hall
CPC分类号： C12Q1/6823 , C12Q2561/109 , C12Q2547/101 , C12Q2531/113
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
摘要翻译： 本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。 本发明还提供了用于组合的靶和信号产生测定的方法。
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公开(公告)号：US20050003432A1
公开(公告)日：2005-01-06
申请号：US10897793
申请日：2004-07-23
申请人： Jeff Hall , Victor Lyamichev , Andrea Mast , Mary Brow , Robert Kwiatkowski , Stephanie Vavra
发明人： Jeff Hall , Victor Lyamichev , Andrea Mast , Mary Brow , Robert Kwiatkowski , Stephanie Vavra
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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公开(公告)号：US20070292856A1
公开(公告)日：2007-12-20
申请号：US11489665
申请日：2006-07-19
申请人： Victor Lyamichev , Michael Kaiser , Natalie Lyamichev , Jeff Hall , James Prudent , Mary Brow
发明人： Victor Lyamichev , Michael Kaiser , Natalie Lyamichev , Jeff Hall , James Prudent , Mary Brow
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6813 , C12Q2521/301
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20070003942A1
公开(公告)日：2007-01-04
申请号：US11183077
申请日：2005-07-15
申请人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Ann Brow , James Dahlberg
发明人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Ann Brow , James Dahlberg
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20060292580A1
公开(公告)日：2006-12-28
申请号：US11183015
申请日：2005-07-15
申请人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Ann Brow , James Dahlberg
发明人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Ann Brow , James Dahlberg
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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