-
公开(公告)号：US07625737B2
公开(公告)日：2009-12-01
申请号：US10980759
申请日：2004-11-03
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The present invention relates to alpha-amylase variants having mutations corresponding to one or more of the following mutations A181E,D,Q,N,V; I201W,F,L; Q264R,D,K,E; Y290R,E,K; and S334A,V,T,L,I,D (using SEQ ID No. 2 for numbering).
摘要翻译： 本发明涉及具有对应于一个或多个以下突变A181E，D，Q，N，V的突变的α-淀粉酶变体; I201W，F，L; Q264R，D，K，E; Y290R，E，K; 和S334A，V，T，L，I，D（使用SEQ ID No.2编号）。
-
公开(公告)号：US20090203106A1
公开(公告)日：2009-08-13
申请号：US12420446
申请日：2009-04-08
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Carsten Ryttergaard , Sine Larsen
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Carsten Ryttergaard , Sine Larsen
IPC分类号： C12N9/24
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： The present invention relates to variants of Glycoside Hydrolase family 53 galactanases, e.g., variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 本发明涉及糖苷水解酶家族53半乳聚糖酶的变体，例如来自耶尔森氏菌，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或黄单胞菌属的半乳聚糖酶的变体。
-
公开(公告)号：US06361989B1
公开(公告)日：2002-03-26
申请号：US09290734
申请日：1999-04-13
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
IPC分类号： C12N900
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
-
公开(公告)号：US6022724A
公开(公告)日：2000-02-08
申请号：US683838
申请日：1996-07-18
IPC分类号： C12N15/09 , C11D3/386 , C12N1/21 , C12N9/28 , C12N15/00 , C12N15/56 , C12R1/07 , C11D3/00 , C12N9/30
CPC分类号： C12N9/2417 , C11D3/386
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprisesi) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations),ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally,iii) testing the resulting Termamyl-like .alpha.-amylase variant with respect to the property in question.
摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与亲本α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。
-
公开(公告)号：US20120283164A1
公开(公告)日：2012-11-08
申请号：US13556623
申请日：2012-07-24
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Neilsen , Lisbeth Hedegaard
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
-
公开(公告)号：US20110251117A1
公开(公告)日：2011-10-13
申请号：US13161931
申请日：2011-06-16
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.
摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。
-
公开(公告)号：US07960161B2
公开(公告)日：2011-06-14
申请号：US11862580
申请日：2007-09-27
IPC分类号： C12N9/24
CPC分类号： C12N9/2417 , C11D3/386 , C12P19/02 , C12P19/14
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+dependency and specific activity.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。
-
公开(公告)号：US20090263881A1
公开(公告)日：2009-10-22
申请号：US11953532
申请日：2007-12-10
申请人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
发明人： Torben Vedel Borchert , Allan Svendsen , Carsten Andersen , Bjarne Nielsen , Torben Lauesgaard Nissen , Soren Kjaerulff
IPC分类号： C12N9/26
摘要： The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
摘要翻译： 本发明涉及亲本的类戊酰样α-淀粉酶的变体，其相对于所述亲本α-淀粉酶表现出至少一种以下特性的改变：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。
-
公开(公告)号：US07378264B2
公开(公告)日：2008-05-27
申请号：US11064196
申请日：2005-02-22
CPC分类号： C11D3/386 , C12N9/2417
摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.
摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。
-
公开(公告)号：US06528298B1
公开(公告)日：2003-03-04
申请号：US09545586
申请日：2000-04-07
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
IPC分类号： C12P2106
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
-
-
-
-
-
-
-
-
-
搜索结果
256 条记录 (耗时 0.046 秒)
