公开(公告)号：US20210292822A1

公开(公告)日：2021-09-23

申请号：US17237670

申请日：2021-04-22

Applicant: Spatial Transcriptomics AB ,  Illumina, Inc.

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC: C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876

Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US10975371B2

公开(公告)日：2021-04-13

申请号：US14855207

申请日：2015-09-15

Applicant: ILLUMINA, INC.

Inventor： Neeraj Salathia ,  Jian-Bing Fan ,  Fiona Kaper ,  Gordon M. Cann ,  Arash Jamshidi ,  Alex Aravanis

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6853 ,  C12Q1/6806

Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

公开(公告)号：US10781485B2

公开(公告)日：2020-09-22

申请号：US15959093

申请日：2018-04-20

Applicant: Illumina, Inc.

Inventor： AmirAli Hajhossein Talasaz ,  Gordon M. Cann

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6869 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6827

Abstract: Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.

公开(公告)号：US10774374B2

公开(公告)日：2020-09-15

申请号：US15565637

申请日：2016-04-04

Applicant: Spatial Transcriptomics AB ,  ILLUMINA, INC.

Inventor： Jonas Frisén ,  Patrik Ståhl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC: C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876

Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US10457969B2

公开(公告)日：2019-10-29

申请号：US14804068

申请日：2015-07-20

Applicant: Illumina, Inc.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.