公开(公告)号：US11479809B2

公开(公告)日：2022-10-25

申请号：US17704830

申请日：2022-03-25

Applicant: Spatial Transcriptomics AB

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC: C12Q1/682 ,  G01N1/30 ,  G01N1/42 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6844 ,  G16B50/30 ,  G16B50/20 ,  G16B30/00 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12N15/10

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US11162132B2

公开(公告)日：2021-11-02

申请号：US17237670

申请日：2021-04-22

Applicant: Spatial Transcriptomics AB ,  Illumina, Inc.

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC: C12Q1/6834 ,  C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6874

Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US11613773B2

公开(公告)日：2023-03-28

申请号：US17834474

申请日：2022-06-07

Applicant: Spatial Transcriptomics AB ,  Illumina, Inc.

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg ,  Gordon M. Cann ,  Leila Bazargan ,  Alex Aravanis

IPC: C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12N15/10

Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US11352659B2

公开(公告)日：2022-06-07

申请号：US17474922

申请日：2021-09-14

Applicant: Spatial Transcriptomics AB

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC: C12Q1/682 ,  G01N1/30 ,  G01N1/42

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20220127659A1

公开(公告)日：2022-04-28

申请号：US17474922

申请日：2021-09-14

Applicant: Spatial Transcriptomics AB

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC: C12Q1/682 ,  G01N1/30 ,  G01N1/42

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20210214785A1

公开(公告)日：2021-07-15

申请号：US17147874

申请日：2021-01-13

Applicant: Spatial Transcriptomics AB

Inventor： Marlon Stoeckius

IPC: C12Q1/6874 ,  C12Q1/6837

Abstract: Provided herein are methods of determining a location of a target analyte in a non-permeabilized biological sample and methods of reducing background binding of an analyte on an array.

公开(公告)号：US20220090058A1

公开(公告)日：2022-03-24

申请号：US17474899

申请日：2021-09-14

Applicant: Spatial Transcriptomics AB

Inventor： Jonas Frisen ,  Patrik Stahl ,  Joakim Lundeberg

IPC: C12N15/10

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20210285046A1

公开(公告)日：2021-09-16

申请号：US17220534

申请日：2021-04-01

Applicant: 10x Genomics, Inc. ,  Spatial Transcriptomics AB

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Felice Alessio Bava ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20190024154A1

公开(公告)日：2019-01-24

申请号：US16043038

申请日：2018-07-23

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： JONAS FRISEN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG

IPC: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6841

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20190017106A1

公开(公告)日：2019-01-17

申请号：US16013654

申请日：2018-06-20

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： JONAS FRISEN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG

IPC: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6841

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.