公开(公告)号：US20070026479A1

公开(公告)日：2007-02-01

申请号：US11475260

申请日：2006-06-27

申请人： Thomas Beardslee ,  Cinnamon Bolz ,  Timothy Updyke ,  Regina Rooney

发明人： Thomas Beardslee ,  Cinnamon Bolz ,  Timothy Updyke ,  Regina Rooney

IPC分类号： C12Q1/37 ,  C07K1/26

CPC分类号： G01N27/44704 ,  C07K1/16 ,  C07K1/26

摘要： Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at −20 degrees C. Methods of using the markers and kits containing stable native protein markers in liquid form for determining molecular mass of proteins using electrophoresis are also provided. Furthermore, methods for generating revenue by selling the liquid molecular weight markers, are provided.

摘要翻译： 标记组提供用于非变性凝胶。 蛋白质分子量标记物以液体形式提供，并且在4℃下以液体形式稳定至少两个月，在-20℃下至少一年。使用含有稳定天然蛋白质标记物的标记物和试剂盒的方法 还提供用于使用电泳测定蛋白质分子量的液体形式。 此外，提供了通过销售液体分子量标记来产生收入的方法。

公开(公告)号：USD695418S1

公开(公告)日：2013-12-10

申请号：US29429757

申请日：2012-08-16

申请人： Timothy Updyke ,  Paul Predki ,  Evangeline Gonzalez ,  Randall Lowe ,  Tad Simons

设计人： Timothy Updyke ,  Paul Predki ,  Evangeline Gonzalez ,  Randall Lowe ,  Tad Simons

公开(公告)号：US20120234678A1

公开(公告)日：2012-09-20

申请号：US13392493

申请日：2009-09-03

申请人： Thomas Diller ,  Timothy Updyke

发明人： Thomas Diller ,  Timothy Updyke

IPC分类号： G01N21/64 ,  G01N27/447

CPC分类号： G01N33/6803 ,  B33Y80/00 ,  C07K1/26 ,  Y10T436/10 ,  Y10T436/105831

摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.

摘要翻译： 本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。 本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。 进一步的实施方案涉及使用双重标记的蛋白质标准物来更准确地测定电泳泳道中存在的蛋白质的量。 另外的实施方案涉及包含目前描述的双重蛋白质标准品的试剂盒。 根据本文所述的实施方案制备和使用的双重标记的蛋白质标准物可用于同时确定受试蛋白质的分子量和相对量。

公开(公告)号：US20070151853A1

公开(公告)日：2007-07-05

申请号：US11614785

申请日：2006-12-21

申请人： Thomas Beardslee ,  Timothy Updyke

发明人： Thomas Beardslee ,  Timothy Updyke

IPC分类号： C07K1/26 ,  G01N27/00

CPC分类号： G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

摘要： Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

摘要翻译： 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。