利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

34 条记录 (耗时 0.033 秒)

    Isopentenyl diphosphate isomerase from Hevea brasiliensis and rubber producing method using the same
    22.
    发明授权
    Isopentenyl diphosphate isomerase from Hevea brasiliensis and rubber producing method using the same 失效
    来自三叶草的异戊烯二磷酸异构酶和使用其的橡胶生产方法

    公开(公告)号：US06316695B1

    公开(公告)日：2001-11-13

    申请号：US09296754

    申请日：1999-04-22

    申请人： Kyung-Han Han ,  Hun-Seung Kang ,  Soo-Kyung Oh ,  Dong-Ho Shin ,  Jae-Mo Yang

    发明人： Kyung-Han Han ,  Hun-Seung Kang ,  Soo-Kyung Oh ,  Dong-Ho Shin ,  Jae-Mo Yang

    IPC分类号： C12N1582

    CPC分类号： C08F136/08 ,  C12N9/90 ,  C12N15/8243 ,  C12P5/007 ,  Y10S435/814

    摘要： The present invention cloned a cDNA clone encoding isopentenyl diphosphate (hereafter “IPP”) isomerase (EC 5.3.3.2) from a cDNA library of Hevea brasiliensis latex. The clone has a continuous open reading frame encoding a peptide of 234 amino acids with a predicted molecular mass of 26.7 kDa. The deduced protein is acidic with an isoelectric point of 4.7 and shows high sequence identity with other IPP isomerases. The recombinant protein expressed in Escherichia coli showed IPP isomerase activity. In vitro rubber biosynthesis assays using washed rubber particle (WRP) deprived of initiating allylic diphosphates were performed with the addition of IPP isomerase in the reaction mixture. Results revealed that the recombinant IPP isomerase is catalytically active in catalyzing the conversion of IPP to DMAPP, a key activation step of the basic five-carbon isoprene unit in rubber biosynthesis. Southern analysis indicated that the IPP isomerase is encoded by two genes in Hevea rubber tree. In Northern blot analysis, two different sizes of transcripts (1.2 and 0.6 kb) were detected from leaf tissues while only one hybridizing band (1.0 kb) was detected from latex. Analyses of RNA extracted from extruded latex and leaf tissues of the trees wounded with nails showed that wounding did not change the transcript level of IPP isomerase.

    摘要翻译： 本发明克隆了编码异戊烯基二磷酸（以下称为“IPP”）异构酶的cDNA克隆（EC 5.3.3.2），其从巴西三叶胶乳的cDNA文库中克隆。 克隆具有编码234个氨基酸的肽的连续开放阅读框，其预测分子量为26.7kDa。 推测的蛋白质为酸性，等电点为4.7，与其他IPP异构酶显示高序列同一性。 在大肠杆菌中表达的重组蛋白显示IPP异构酶活性。 在反应混合物中加入IPP异构酶，使用剥夺了引发烯丙基二磷酸酯的洗涤橡胶颗粒（WRP）进行体外橡胶生物合成测定。 结果表明，重组IPP异构酶在催化IPP转化为DMAPP时具有催化活性，是橡胶生物合成中基本五碳异戊二烯单元的关键活化步骤。 南方分析表明IPP异构酶由双叶橡胶树中的两个基因编码。 在Northern印迹分析中，从叶组织中检测到两种不同大小的转录物（1.2和0.6kb），而从胶乳中仅检测到一个杂交带（1.0kb）。 用指甲树的挤出乳胶和叶组织提取的RNA的分析显示，伤口没有改变IPP异构酶的转录水平。

    Optical waveguide
    25.
    发明申请
    Optical waveguide 失效
    光波导

    公开(公告)号：US20080056660A1

    公开(公告)日：2008-03-06

    申请号：US11772651

    申请日：2007-07-02

    申请人： Gwan-Su LEE ,  Soo-Jin Jung ,  Dong-Ho Shin ,  Won-Hoe Koo ,  Il-Kweon Joung

    发明人： Gwan-Su LEE ,  Soo-Jin Jung ,  Dong-Ho Shin ,  Won-Hoe Koo ,  Il-Kweon Joung

    IPC分类号： G02B6/10

    CPC分类号： G02B6/1226 ,  B82Y20/00

    摘要： An optical waveguide comprises a linear metallic thin film having a light delivering unit coupled to one end thereof, through which light is incident; and at least more than two dielectric layers surrounding the metallic thin film. In the optical waveguide, an effective refractive index and a propagation loss in the metallic thin film is reduced so that long-range light transmission is realized. Further, a loss of light caused by the coupling to optical fiber, which is coupled to one end of the metallic thin film, is simultaneously reduced.

    摘要翻译： 光波导包括具有耦合到其一端的光输送单元的线性金属薄膜，光入射到该光导体单元; 以及围绕金属薄膜的至少两个以上的电介质层。 在光波导中，金属薄膜的有效折射率和传播损耗减小，从而实现远距离透光。 此外，耦合到金属薄膜的一端的与光纤的耦合引起的光的损失同时减少。

    Apparatus and method for providing sound in a mobile communication terminal
    27.
    发明申请
    Apparatus and method for providing sound in a mobile communication terminal 审中-公开
    一种用于在移动通信终端中提供声音的装置和方法

    公开(公告)号：US20050130716A1

    公开(公告)日：2005-06-16

    申请号：US11009880

    申请日：2004-12-10

    申请人： Dong-Ho Shin ,  Wan-Kyu Park

    发明人： Dong-Ho Shin ,  Wan-Kyu Park

    IPC分类号： H04R1/02 ,  H04B1/00 ,  H04B1/38 ,  H04B7/26 ,  H04M1/00 ,  H04M1/02 ,  H04M1/03 ,  H04R1/34 ,  H04R5/02

    CPC分类号： H04M1/035

    摘要： An apparatus and method for providing sound in a mobile communication device using a sound output device capable of projecting sound in more than one direction such that a the user can recognize all the sound projected by the sound output unit and in a manner capable of enhancing low frequency sound reproduction. A plurality of air ducts connected to sound emitting ports are provided such that the sound projected in a plurality of directions by a speaker is emitted through the same surface of the device. By providing a curved shape for the air ducts that guide the sound projected in one of the plurality of directions, low frequency sound is filtered and enhanced.

    摘要翻译： 一种用于在移动通信设备中使用能够在多个方向上投射声音的声音输出设备提供声音的设备和方法，使得用户可以识别由声音输出单元投射的所有声音并且以能够增强低音的方式 频率声音再现。 设置连接到声音发射端口的多个风道，使得通过扬声器在多个方向上投影的声音通过装置的相同表面发射。 通过为引导在多个方向中的一个方向上突出的声音的导风管提供弯曲形状，对低频声音进行滤波和增强。

    Lab-on-a-chip and method of driving the same
    28.
    发明授权
    Lab-on-a-chip and method of driving the same 有权
    芯片实验室及其驱动方法

    公开(公告)号：US08394595B2

    公开(公告)日：2013-03-12

    申请号：US12738683

    申请日：2008-10-17

    申请人： Moon-Youn Jung ,  Dong-Ho Shin ,  Minsuk Jeong ,  Sanghee Kim ,  Hye-Yoon Kim ,  Seon-Hee Park

    发明人： Moon-Youn Jung ,  Dong-Ho Shin ,  Minsuk Jeong ,  Sanghee Kim ,  Hye-Yoon Kim ,  Seon-Hee Park

    IPC分类号： G01N33/53

    CPC分类号： G01N33/49

    摘要： Provided is a lab-on-a-chip. The lab-on-a-chip includes a first region where a lower substrate and an upper substrate are bonded to each other, a second region where the lower and upper substrates are not bonded, and a gap adjusting member disposed at an end of the second region that is opposite to a boundary between the first and second regions, the gap adjusting member being configured to adjust a gap between the first and second substrates to control a capillary force.

    摘要翻译： 提供了一个芯片实验室。 芯片实验室包括下基板和上基板彼此接合的第一区域，下基板和上基板未接合的第二区域，以及设置在该基板的末端的间隙调节构件 所述第二区域与所述第一和第二区域之间的边界相对，所述间隙调节构件被配置为调节所述第一和第二基板之间的间隙以控制毛细管力。

    Optical waveguide
    30.
    发明授权
    Optical waveguide 失效
    光波导

    公开(公告)号：US07496264B2

    公开(公告)日：2009-02-24

    申请号：US11772651

    申请日：2007-07-02

    申请人： Gwan-Su Lee ,  Soo-Jin Jung ,  Dong-Ho Shin ,  Won-Hoe Koo ,  Il-Kweon Joung

    发明人： Gwan-Su Lee ,  Soo-Jin Jung ,  Dong-Ho Shin ,  Won-Hoe Koo ,  Il-Kweon Joung

    IPC分类号： G02B6/10

    CPC分类号： G02B6/1226 ,  B82Y20/00

    摘要： An optical waveguide includes a linear metallic thin film having a light delivering unit coupled to one end thereof, through which light is incident; and at least more than two dielectric layers surrounding the metallic thin film. In the optical waveguide, an effective refractive index and a propagation loss in the metallic thin film is reduced so that long-range light transmission is realized. Further, a loss of light caused by the coupling to optical fiber, which is coupled to one end of the metallic thin film, is simultaneously reduced.

    摘要翻译： 光波导包括具有耦合到其一端的光输送单元的线性金属薄膜，光入射到该光导体单元; 以及围绕金属薄膜的至少两个以上的电介质层。 在光波导中，金属薄膜的有效折射率和传播损耗减小，从而实现远距离透光。 此外，耦合到金属薄膜的一端的与光纤的耦合引起的光的损失同时减少。