公开(公告)号：US20070299244A1

公开(公告)日：2007-12-27

申请号：US11652865

申请日：2007-01-12

申请人： Shinji Chaki ,  Shigetada Chaki ,  Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima

发明人： Shinji Chaki ,  Shigetada Chaki ,  Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima

IPC分类号： C07K16/18 ,  C07H21/04 ,  C07K14/00

CPC分类号： A61K9/127 ,  A61K38/28 ,  C07K1/00 ,  C07K5/00 ,  C07K7/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  C07K19/00

摘要： With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.

摘要翻译： 为了提供脂质体，其具有引入脂质体膜的外表面的亲水性聚合物，脂质体膜是能够使脂质体包裹的物质从内体中逸出并释放到细胞质中的脂质体，脂质体膜组分 与下文（a）或（b）所示的肽结合，并且与另一端与下文（a）或（b）所示的肽结合的亲水性聚合物的一端结合的脂质体膜组分包括在 脂质体：（a）包含SEQ ID NO：1的氨基酸序列的肽; （b）包含SEQ ID NO：1的氨基酸序列的肽，其中1个或多个缺失，替换或添加的氨基酸，并且能够在酸性条件下彼此融合脂质膜。

公开(公告)号：US08592210B2

公开(公告)日：2013-11-26

申请号：US11396081

申请日：2006-03-31

申请人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

发明人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

IPC分类号： A61K48/00 ,  A61K9/127 ,  C12N15/88

CPC分类号： C12N15/88 ,  A61K9/1273 ,  A61K47/34 ,  A61K47/645 ,  A61K47/6911 ,  C07K1/1077

摘要： With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.

摘要翻译： 为了提供具有细胞和核进入能力的脂质体，为了达到这个目的，提供了一种脂质体，其表面上含有多个连续的精氨酸残基的肽，特别是提供一种脂质体，其中肽用疏水基团修饰 或疏水性化合物，并且将疏水基团或疏水性化合物插入脂质双层中，使得该肽暴露在双层的表面上。

公开(公告)号：US20100104623A1

公开(公告)日：2010-04-29

申请号：US12449817

申请日：2008-02-27

申请人： Hideyoshi Harashima ,  Kentaro Kogure ,  Hidetaka Akita ,  Akitada Iwasa ,  Yoshio Nakamura ,  Shiroh Futaki

发明人： Hideyoshi Harashima ,  Kentaro Kogure ,  Hidetaka Akita ,  Akitada Iwasa ,  Yoshio Nakamura ,  Shiroh Futaki

IPC分类号： A61K9/127

CPC分类号： A61K9/127 ,  A61K38/00 ,  A61K47/42 ,  C07K14/00

摘要： It is intended to provide a positively charged liposome, particularly having a polyarginine peptide on a surface thereof, which is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood. An agent for enhancing the resistance of liposome against biological component comprising a peptide having at least one of the following characteristics (a) and (b) as an active ingredient: (a) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2; and (b) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2, in which one or more amino acids are deleted, substituted or added, and having an activity for promoting lipid membrane fusion under acidic condition. An agent for enhancing the resistance of liposome against biological component in this invention, being included in a liposome, is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood.

摘要翻译： 旨在提供带正电荷的脂质体，特别是在其表面具有聚精氨酸肽，其能够增加对带负电的生物成分如血液中的蛋白质的抗性，并且保持高的物质输送能力 在血中 用于增强脂质体对生物成分的抗性的试剂，其包含具有以下特征（a）和（b）中的至少一个作为活性成分的肽：（a）包含SEQ ID No： 1或2; 和（b）包含SEQ ID No：1或2所示的氨基酸序列的肽，其中一个或多个氨基酸缺失，取代或添加，并且具有在酸性条件下促进脂质膜融合的活性。 包含在脂质体中的用于增强本发明中脂质体对抗生物成分的抗性的药剂能够增加对带负电的生物成分如血液中的蛋白质的抗性，并保持高的物质输送能力 在血中

公开(公告)号：US20080175895A1

公开(公告)日：2008-07-24

申请号：US11962011

申请日：2007-12-20

申请人： Kentaro Kogure ,  Moeko Miyashita ,  Hideyoshi Harashima

发明人： Kentaro Kogure ,  Moeko Miyashita ,  Hideyoshi Harashima

IPC分类号： A61K9/127 ,  A61K38/44 ,  A61P25/28 ,  A61P25/16 ,  A61P35/00 ,  A61P17/00

CPC分类号： A61K9/127 ,  A61K8/14 ,  A61K8/66 ,  A61K38/44 ,  A61K38/446 ,  A61K2800/522 ,  A61Q17/04 ,  A61Q19/004 ,  A61Q19/08 ,  C12Y111/01006 ,  C12Y111/01009 ,  C12Y115/01001

摘要： Systems, devices, and methods for delivering one or more active ingredients to intradermal tissues, deep regions of pores, and intradermal tissues in the vicinity of the pores. In some embodiments, a composition is provided including a plurality of liposomes including a cationic lipid, and an amphiphilic glycerophospholipid having a saturated fatty acid moiety and an unsaturated fatty acid moiety, and one or more antioxidants and/or antioxidant enzymes.

摘要翻译： 用于将一种或多种活性成分递送至孔附近的真皮内组织，毛孔深部和皮内组织的系统，装置和方法。 在一些实施方案中，提供了包含多个包含阳离子脂质的脂质体和具有饱和脂肪酸部分和不饱和脂肪酸部分的两亲性甘油磷脂以及一种或多种抗氧化剂和/或抗氧化酶的组合物。