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    Liposome allowing liposome-entrapped substance to escape from endosome
    1.
    发明授权
    Liposome allowing liposome-entrapped substance to escape from endosome 有权
    脂质体允许脂质体包裹的物质从内体逃逸

    公开(公告)号：US08946380B2

    公开(公告)日：2015-02-03

    申请号：US11652865

    申请日：2007-01-12

    申请人： Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima ,  Shigetada Chaki

    发明人： Shinji Chaki ,  Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima

    IPC分类号： A61K38/28 ,  C07K1/00 ,  A61K9/127 ,  C07K5/00 ,  C07K19/00 ,  C07K17/00 ,  C07K14/00 ,  C07K7/00 ,  C07K16/00

    CPC分类号： A61K9/127 ,  A61K38/28 ,  C07K1/00 ,  C07K5/00 ,  C07K7/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  C07K19/00

    摘要： With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.

    摘要翻译： 为了提供脂质体，其具有引入脂质体膜的外表面的亲水性聚合物，脂质体膜是能够使脂质体包裹的物质从内体中逸出并释放到细胞质中的脂质体，脂质体膜组分 与下文（a）或（b）所示的肽结合，并且与另一端与下文（a）或（b）所示的肽结合的亲水性聚合物的一端结合的脂质体膜组分包括在 脂质体：（a）包含SEQ ID NO：1的氨基酸序列的肽; （b）包含SEQ ID NO：1的氨基酸序列的肽，其中1个或多个缺失，替换或添加的氨基酸，并且能够在酸性条件下彼此融合脂质膜。

    Liposome Capable of Effective Delivery of Given Substance Into Nucleus
    2.
    发明申请
    Liposome Capable of Effective Delivery of Given Substance Into Nucleus 审中-公开
    能够有效递送给予物质的脂质体

    公开(公告)号：US20090305409A1

    公开(公告)日：2009-12-10

    申请号：US11887030

    申请日：2006-03-24

    申请人： Kentaro Kogure ,  Takashi Nakamura ,  Hidetaka Akita ,  Hideyoshi Harashima ,  Shiroh Futaki

    发明人： Kentaro Kogure ,  Takashi Nakamura ,  Hidetaka Akita ,  Hideyoshi Harashima ,  Shiroh Futaki

    IPC分类号： C12N5/00

    CPC分类号： C12N15/88 ,  A61K48/00

    摘要： Disclosed is a non-viral vector capable of delivering a given substance into the nucleus of a target cell effectively even when the target cells is a undividable cell such as a dendritic cell. A bilamellar liposome having a first lipid membrane and a second lipid membrane successively from the outside, the first lipid membrane having a membrane-fusing ability and the second lipid membrane having on its surface a nuclear transport peptide.

    摘要翻译： 公开了即使当靶细胞是不可分割的细胞例如树突状细胞时，能够有效地将给定物质有效地递送到靶细胞的细胞核中的非病毒载体。 具有从外部连续地具有第一脂质膜和第二脂质膜的第二脂质膜，所述第一脂质膜具有膜融合能力，所述第二脂质膜在其表面上具有核转运肽。

    Liposome allowing liposome-entrapped substance to escape from endosome
    3.
    发明申请
    Liposome allowing liposome-entrapped substance to escape from endosome 有权
    脂质体允许脂质体包裹的物质从内体逃逸

    公开(公告)号：US20070299244A1

    公开(公告)日：2007-12-27

    申请号：US11652865

    申请日：2007-01-12

    申请人： Shinji Chaki ,  Shigetada Chaki ,  Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima

    发明人： Shinji Chaki ,  Shigetada Chaki ,  Kentaro Kogure ,  Shiroh Futaki ,  Hideyoshi Harashima

    IPC分类号： C07K16/18 ,  C07H21/04 ,  C07K14/00

    CPC分类号： A61K9/127 ,  A61K38/28 ,  C07K1/00 ,  C07K5/00 ,  C07K7/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  C07K19/00

    摘要： With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.

    摘要翻译： 为了提供脂质体，其具有引入脂质体膜的外表面的亲水性聚合物，脂质体膜是能够使脂质体包裹的物质从内体中逸出并释放到细胞质中的脂质体，脂质体膜组分 与下文（a）或（b）所示的肽结合，并且与另一端与下文（a）或（b）所示的肽结合的亲水性聚合物的一端结合的脂质体膜组分包括在 脂质体：（a）包含SEQ ID NO：1的氨基酸序列的肽; （b）包含SEQ ID NO：1的氨基酸序列的肽，其中1个或多个缺失，替换或添加的氨基酸，并且能够在酸性条件下彼此融合脂质膜。

    Polyarginine-modified liposome having nuclear entry ability
    4.
    发明授权
    Polyarginine-modified liposome having nuclear entry ability 失效
    具有核进入能力的聚精氨酸改性脂质体

    公开(公告)号：US08592210B2

    公开(公告)日：2013-11-26

    申请号：US11396081

    申请日：2006-03-31

    申请人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

    发明人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

    IPC分类号： A61K48/00 ,  A61K9/127 ,  C12N15/88

    CPC分类号： C12N15/88 ,  A61K9/1273 ,  A61K47/34 ,  A61K47/645 ,  A61K47/6911 ,  C07K1/1077

    摘要： With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.

    摘要翻译： 为了提供具有细胞和核进入能力的脂质体，为了达到这个目的，提供了一种脂质体，其表面上含有多个连续的精氨酸残基的肽，特别是提供一种脂质体，其中肽用疏水基团修饰 或疏水性化合物，并且将疏水基团或疏水性化合物插入脂质双层中，使得该肽暴露在双层的表面上。

    AGENT FOR ENHANCING THE RESISTANCE OF LIPOSOME AGAINST BIOLOGICAL COMPONENT, AND LIPOSOME MODIFIED WITH THE AGENT
    5.
    发明申请
    AGENT FOR ENHANCING THE RESISTANCE OF LIPOSOME AGAINST BIOLOGICAL COMPONENT, AND LIPOSOME MODIFIED WITH THE AGENT 审中-公开
    用于增强生物组分的脂质体抗性的试剂和用该试剂修饰的脂质体

    公开(公告)号：US20100104623A1

    公开(公告)日：2010-04-29

    申请号：US12449817

    申请日：2008-02-27

    申请人： Hideyoshi Harashima ,  Kentaro Kogure ,  Hidetaka Akita ,  Akitada Iwasa ,  Yoshio Nakamura ,  Shiroh Futaki

    发明人： Hideyoshi Harashima ,  Kentaro Kogure ,  Hidetaka Akita ,  Akitada Iwasa ,  Yoshio Nakamura ,  Shiroh Futaki

    IPC分类号： A61K9/127

    CPC分类号： A61K9/127 ,  A61K38/00 ,  A61K47/42 ,  C07K14/00

    摘要： It is intended to provide a positively charged liposome, particularly having a polyarginine peptide on a surface thereof, which is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood. An agent for enhancing the resistance of liposome against biological component comprising a peptide having at least one of the following characteristics (a) and (b) as an active ingredient: (a) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2; and (b) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2, in which one or more amino acids are deleted, substituted or added, and having an activity for promoting lipid membrane fusion under acidic condition. An agent for enhancing the resistance of liposome against biological component in this invention, being included in a liposome, is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood.

    摘要翻译： 旨在提供带正电荷的脂质体，特别是在其表面具有聚精氨酸肽，其能够增加对带负电的生物成分如血液中的蛋白质的抗性，并且保持高的物质输送能力 在血中 用于增强脂质体对生物成分的抗性的试剂，其包含具有以下特征（a）和（b）中的至少一个作为活性成分的肽：（a）包含SEQ ID No： 1或2; 和（b）包含SEQ ID No：1或2所示的氨基酸序列的肽，其中一个或多个氨基酸缺失，取代或添加，并且具有在酸性条件下促进脂质膜融合的活性。 包含在脂质体中的用于增强本发明中脂质体对抗生物成分的抗性的药剂能够增加对带负电的生物成分如血液中的蛋白质的抗性，并保持高的物质输送能力 在血中

    Polyarginine-modified liposome having nuclear entry ability
    6.
    发明申请
    Polyarginine-modified liposome having nuclear entry ability 失效
    具有核进入能力的聚精氨酸改性脂质体

    公开(公告)号：US20070059353A1

    公开(公告)日：2007-03-15

    申请号：US11396081

    申请日：2006-03-31

    申请人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

    发明人： Hideyoshi Harashima ,  Shiroh Futaki ,  Kentaro Kogure

    IPC分类号： A61K48/00 ,  A61K9/127 ,  C12N15/88

    CPC分类号： C12N15/88 ,  A61K9/1273 ,  A61K47/34 ,  A61K47/645 ,  A61K47/6911 ,  C07K1/1077

    摘要： With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.

    摘要翻译： 为了提供具有细胞和核进入能力的脂质体，为了达到这个目的，提供了一种脂质体，其表面上含有多个连续的精氨酸残基的肽，特别是提供一种脂质体，其中肽用疏水基团修饰 或疏水性化合物，并且将疏水基团或疏水性化合物插入脂质双层中，使得该肽暴露在双层的表面上。

    METHOD OF PRODUCING MICROCAPSULES
    9.
    发明申请
    METHOD OF PRODUCING MICROCAPSULES 有权
    生产微生物的方法

    公开(公告)号：US20100143482A1

    公开(公告)日：2010-06-10

    申请号：US12513237

    申请日：2007-11-02

    申请人： Yoshinobu Baba ,  Manabu Tokeshi ,  Noritada Kaji ,  Hideyoshi Harashima ,  Kentaro Kogure ,  Yasuo Shinohara ,  Hiroshi Kuramoto

    发明人： Yoshinobu Baba ,  Manabu Tokeshi ,  Noritada Kaji ,  Hideyoshi Harashima ,  Kentaro Kogure ,  Yasuo Shinohara ,  Hiroshi Kuramoto

    IPC分类号： A61K9/50 ,  A61K31/7088

    CPC分类号： A61K48/0008 ,  A61K9/1271 ,  A61K9/1277 ,  A61K9/5169 ,  A61K9/5192 ,  A61K48/0041 ,  B01J13/04 ,  C12N15/87

    摘要： It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.

    摘要翻译： 旨在提供可以少量生产可用于例如基因载体的各种微胶囊的方法。 该制造方法包括：将含有待包封物质的流体在至少一个材料供给微通道的基材中进料，其中这些材料供给微通道（12,13,14）和反应微通道（18） 与这些送料微通道相连; 将含有用于形成信封的材料的包络成形流体进料到至少一个其它材料馈送微通道（12,14）中; 并且允许包络成形流体和含有从每个供料微通道收集到反应微通道中的包封物质的流体流过反应微通道，同时连续地保持在其汇合处形成的流体之间的界面。