公开(公告)号：US10150992B2

公开(公告)日：2018-12-11

申请号：US15202487

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Krishnan Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US20130171715A1

公开(公告)日：2013-07-04

申请号：US13742667

申请日：2013-01-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joe Y.L. Lam ,  Steven M. Menchen ,  Ruiming Zou ,  Scott C. Benson

IPC: C09B11/12 ,  C09B11/06 ,  C09B11/26

CPC classification number: C09B11/24 ,  C07D311/82 ,  C07D491/14 ,  C09B11/06 ,  C09B11/12 ,  C09B11/22 ,  C09B11/26 ,  C09B13/00 ,  C09B57/00 ,  C09B62/0025

Abstract: Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes. The phenyl xanthene dyes are useful for a number of purposes, including labels for use in automated DNA sequencing as well the formation of fluorescent “bar codes” for polymeric particles used in the multiplexed analysis of analytes.

Abstract translation: 描述了包含任何含有特定C 9苯环的任何荧光素，罗丹明或若丹明的荧光苯基呫吨染料。 下C9苯环上的一个或两个邻基被选自烷基，杂烷基，烷氧基，卤素，卤代烷基，氨基，巯基，烷硫基，氰基，异氰基，氰基，巯基氰基，亚硝基，硝基，叠氮基 ，磺酰亚胺基，亚磺酰基和亚磺酰基。 在一个实施方案中，使用卤素和/或羟基。 最佳染料含有较低的C 9苯环，其中两个邻基团相同，并且下环表现出相对于从苯环连接点到呫吨染料的其余部分的假想轴的对称性。 附件点。 苯基呫吨染料可以被活化。 此外，苯基呫吨染料可以与一种或多种包括其它染料的物质共轭。 苯基呫吨染料可用于许多目的，包括用于自动DNA测序的标记，以及用于分析物复用分析中使用的聚合物颗粒的荧光“条形码”。

公开(公告)号：US11702696B2

公开(公告)日：2023-07-18

申请号：US16938222

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US10888831B2

公开(公告)日：2021-01-12

申请号：US16206938

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander Mastroianni ,  Scott C. Benson ,  Steven M. Menchen ,  John Scott

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

公开(公告)号：US10941439B2

公开(公告)日：2021-03-09

申请号：US16176231

申请日：2018-10-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US10143990B2

公开(公告)日：2018-12-04

申请号：US15203582

申请日：2016-07-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander Mastroianni ,  Scott C. Benson ,  Steven M. Menchen ,  John Scott

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

公开(公告)号：US09938577B2

公开(公告)日：2018-04-10

申请号：US14855705

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer H. Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/68 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.