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    Detergent composition containing an alkaline pullulanase from bacillus ferm BP-3048
    22.
    发明授权
    Detergent composition containing an alkaline pullulanase from bacillus ferm BP-3048 失效
    含有来自芽孢杆菌BP-3048的碱性支链淀粉酶的洗涤剂组合物

    公开(公告)号：US5316691A

    公开(公告)日：1994-05-31

    申请号：US960262

    申请日：1992-10-13

    申请人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

    发明人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

    IPC分类号： C11D3/386 ,  C12N9/44 ,  C11D1/00 ,  C11D3/00 ,  C11D17/00

    CPC分类号： C11D3/38636 ,  C12N9/2451 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

    摘要： A detergent composition containing an alkaline pullulanase, a surfactant, alkaline agents and/or inorganic electrolytes, divalent metal ion scavengers and bleaching agents is disclosed. The alkaline pullulanase has an optimum pH range of 8.5-10.0 on pullulan, an optimum temperature of about 50.degree. C. and is not deactivated by surfactants. Further, the pullulanase has a strong resistance to almost all detergent components such as chelating agents, proteases, etc. The pullulanase is isolated from Bacillus sp. KSM-AP 1378 deposited as FERM BP-3048. The composition specifically contains 0.1-10 wt. % alkaline pullulanase B, 0.5-60 wt. % surfactant, 0-90 wt. % alkaline agents and/or inorganic electrolytes, 0-50 wt. % divalent metal ion scavengers, and 0-85 wt. % bleaching agents.

    摘要翻译： 公开了含有碱性支链淀粉酶，表面活性剂，碱剂和/或无机电解质，二价金属离子清除剂和漂白剂的洗涤剂组合物。 碱性支链淀粉酶在支链淀粉上的最适pH范围为8.5-10.0，最适温度为约50℃，不被表面活性剂失活。 此外，支链淀粉酶对几乎所有的洗涤剂组分如螯合剂，蛋白酶等都具有很强的抗性。支链淀粉酶从芽孢杆菌中分离出来。 KSM-AP 1378保存为FERM BP-3048。 该组合物具体含有0.1-10重量％ ％碱性支链淀粉酶B，0.5-60wt。 ％表面活性剂，0-90重量％ ％碱性试剂和/或无机电解质，0-50wt。 ％的二价金属离子清除剂，和0-85wt。 ％漂白剂。

    Modified promoter
    23.
    发明授权
    Modified promoter 有权
    修饰的启动子

    公开(公告)号：US09029519B2

    公开(公告)日：2015-05-12

    申请号：US13499480

    申请日：2010-10-18

    申请人： Akihito Kawahara ,  Hiroshi Kodama ,  Katsutoshi Ara

    发明人： Akihito Kawahara ,  Hiroshi Kodama ,  Katsutoshi Ara

    IPC分类号： C07H21/04 ,  C12N15/75 ,  C12N15/66 ,  C12P21/02 ,  C12N15/11 ,  C12N1/20

    CPC分类号： C12N15/75 ,  C12N1/20 ,  C12N15/11 ,  C12N15/66 ,  C12P21/02

    摘要： The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.

    摘要翻译： 本发明提供一种修饰的启动子，表达载体和各自含有启动子的转化体，以及使用该转化体产生感兴趣的基因产物的方法。 本发明提供一种修饰的启动子，其包含来源于属于芽孢杆菌属的细菌的启动子的核苷酸序列，其中至少一个选自以下的核苷酸序列已被修饰：由SEQ ID NO：1表示的核苷酸序列; 与SEQ ID NO：1所示的核苷酸序列相当的核苷酸序列，除了其中一个或多个碱基被取代，缺失，添加或插入; 和相对于由SEQ ID NO：1表示的核苷酸序列具有70％以上的序列同一性的核苷酸序列。

    Modified microorganism
    25.
    发明授权
    Modified microorganism 有权
    改良微生物

    公开(公告)号：US08236524B2

    公开(公告)日：2012-08-07

    申请号：US12530710

    申请日：2008-04-01

    申请人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

    发明人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

    IPC分类号： C12P21/06 ,  C12N1/00

    CPC分类号： C07K14/32 ,  C12P21/02

    摘要： To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.

    摘要翻译： 提供具有增强的蛋白质或多肽的分泌生产的微生物和使用该微生物产生蛋白质或多肽的方法。 已被遗传修饰以修复SecA的60至80个羧基末端氨基酸的经修饰的微生物。

    Method of modifying target region in host DNA and selectable marker cassette
    29.
    发明授权
    Method of modifying target region in host DNA and selectable marker cassette 有权
    修改宿主DNA和选择性标记盒中靶区的方法

    公开(公告)号：US08852943B2

    公开(公告)日：2014-10-07

    申请号：US12933766

    申请日：2009-03-23

    申请人： Katsutoshi Ara ,  Takuya Morimoto ,  Naotake Ogasawara

    发明人： Katsutoshi Ara ,  Takuya Morimoto ,  Naotake Ogasawara

    IPC分类号： C12N15/70 ,  C12N15/74 ,  C12N15/10 ,  C12N1/21 ,  C07H21/04

    CPC分类号： C12N15/1082 ,  C12N15/102

    摘要： A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter; and a selectable marker cassette for use in the method.

    摘要翻译： 使用供体DNA修饰宿主DNA中的靶区域的方法：其中具有与宿主DNA中的靶区域外侧的5'-侧区域同源的区域的供体DNA，靶标外的3'侧区域 区域，宿主DNA中的靶区域内的第一同源重组区域，并且还具有第一选择标记基因，表达诱导启动子和第二选择标记基因 控制与3'侧区域同源的区域与与第一同源重组区域同源的区域之间的表达诱导启动子; 该方法具有以下步骤：在5'-侧区域和第一同源重组区域的区域上进行供体DNA和宿主DNA之间的同源重组的第一步骤，以进行与供体DNA整合的宿主的选择 基于第一选择标记基因的表达; 以及在源自宿主DNA的3'-侧区域的两个区域和源自供体DNA的3'侧区域之间，通过第1步在与供体DNA整合的宿主DNA内进行同源重组的第2步骤 基于表达诱导启动子的表达诱导条件进行基于第二选择标记基因的表达修饰其靶区的宿主的选择; 以及用于该方法的可选标记盒。