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    Xanthene dyes and their application as luminescence quenching compounds
    21.
    发明授权
    Xanthene dyes and their application as luminescence quenching compounds 有权
    呫吨染料及其作为发光淬灭剂的应用

    公开(公告)号：US06399392B1

    公开(公告)日：2002-06-04

    申请号：US09556464

    申请日：2000-04-21

    Applicant: Richard P. Haugland ,  Victoria L. Singer ,  Stephen T. Yue

    Inventor： Richard P. Haugland ,  Victoria L. Singer ,  Stephen T. Yue

    IPC: G01N2176

    CPC classification number: C07H21/00 ,  C09B11/24 ,  C12Q1/6818 ,  G01N33/542 ,  G01N33/582 ,  Y10S436/80 ,  Y10S436/805

    Abstract: The quenching compounds of the invention are nitrogen-substituted xanthenes that are substituted by one or more aromatic or heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable fluorescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.

    Abstract translation: 本发明的淬灭化合物是被一个或多个芳族或杂芳族猝灭部分取代的氮取代的呫吨。 本发明的淬灭化合物表现出很少或不可观察到的荧光，并有效地淬灭广谱的发光化合物。 化学反应猝灭化合物具有用于标记各种物质（包括生物分子）的效用。 这些标记的物质对于各种能量转移测定和应用是非常有用的。

    Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
    23.
    发明授权
    Dimers of unsymmetrical cyanine dyes containing pyridinium moieties 失效
    含有吡啶鎓部分的不对称花青染料的二聚体

    公开(公告)号：US5410030A

    公开(公告)日：1995-04-25

    申请号：US43665

    申请日：1993-04-05

    Applicant: Stephen T. Yue ,  Richard P. Haugland

    Inventor： Stephen T. Yue ,  Richard P. Haugland

    IPC: C09B23/02 ,  C07H21/02 ,  C07H21/04

    CPC classification number: C09B23/02

    Abstract: The invention relates to nucleic acid stains that are homo- or heterodimers of unsymmetrical cyanine dyes, where at least one dye unit of the dimer is a substituted or unsubstituted benzazolium- or naphthazolium-pyridinium derivative, which dye unit is linked to another unsymmetrical cyanine dye unit, that is the same or different by an aliphatic chain containing one or more heteroatoms; such that the fluorescence of the dye molecules, when bound to nucleic acids, is enhanced. These dimers of unsymmetrical cyanine dyes have spectral properties and binding affinities superior to related monomers. In addition, the dyes can be tailored to absorb and emit in a wide range of wavelengths, which can be further varied by energy transfer mixtures, making the dyes useful for applications that involve simultaneous use of multiple dyes.

    Abstract translation: 本发明涉及作为不对称花青染料的同二聚体或异二聚体的核酸染色，其中二聚体的至少一个染料单元是取代或未取代的苯并唑鎓或萘并吡唑鎓 - 吡啶鎓衍生物，该染料单元与另一不对称花青染料 单元，由含有一个或多个杂原子的脂族链相同或不同; 使得当与核酸结合时染料分子的荧光增强。 这些不对称花青染料的二聚体具有优于相关单体的光谱性质和结合亲和力。 此外，染料可以被定制为在宽范围的波长下吸收和发射，这可以通过能量转移混合物进一步变化，使得染料可用于涉及同时使用多种染料的应用。

    Enzymatic analysis using substrates that yield fluorescent precipitates
    24.
    发明授权
    Enzymatic analysis using substrates that yield fluorescent precipitates 失效
    使用产生荧光沉淀物的底物的酶分析

    公开(公告)号：US5316906A

    公开(公告)日：1994-05-31

    申请号：US748860

    申请日：1991-08-23

    Applicant: Richard P. Haugland ,  Yu-zhong Zhang ,  Stephen T. Yue ,  Ewald Terpetschnig ,  Nels A. Olson ,  John J. Naleway ,  Karen D. Larison ,  Zhijian Huang

    Inventor： Richard P. Haugland ,  Yu-zhong Zhang ,  Stephen T. Yue ,  Ewald Terpetschnig ,  Nels A. Olson ,  John J. Naleway ,  Karen D. Larison ,  Zhijian Huang

    IPC: C12Q1/00 ,  C07D215/14 ,  C07D235/18 ,  C07D239/91 ,  C07D263/56 ,  C07D263/57 ,  C07D277/64 ,  C07D277/66 ,  C07F9/6512 ,  C07F9/6541 ,  C07H15/04 ,  C07H15/26 ,  C07K4/00 ,  C08B37/00 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/44 ,  C12Q1/68 ,  A61K37/02 ,  C04B35/00 ,  C08G63/48

    CPC classification number: C07D263/57 ,  C07D235/18 ,  C07D239/91 ,  C07D277/66 ,  C07F9/65128 ,  C07F9/6541 ,  C07H15/26 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2334/00

    Abstract: Novel fluorescent precipitating substrates made from a class of fluorophores, generally including quinazolinones (quinazolones), benzimidazoles, benzothiazoles, benzoxazoles, quinolines, indolines, and phenanthridines, having the general formula: ##STR1## where carbon atoms of --C.sup.1 .dbd.C.sup.2 -- are further joined so as to complete a first 5- or 6-membered aromatic ring which may contain at least one of the hetero atoms N, O or S,where carbon atoms of --C.sup.4 --N.dbd.C.sup.3 -- are further joined so as to complete a second 5- or 6-membered aromatic ring that contains at least the nitrogen between C.sup.3 and C.sup.4 and may contain at least one additional hetero atom N, O or S,where the first and second aromatic rings may be joined by a 5- or 6-membered bridging ring that contains at least the C.sup.2 from the first aromatic ring and the C.sup.3 from the second aromatic ring, which bridging ring may be saturated or unsaturated and may contain a hetero atom N, O, or S,where each of the first and second aromatic rings may be fused to at least one additional aromatic ring that may contain at least one of the hetero atoms N, O or S, andwhere each of said aromatic rings may be further modified by substitution of any hydrogens on an aromatic carbon by substituents that are halogen, nitro, cyano, aryl, lower alkyl (1-4 carbons), perfluoroalkyl (1-4 carbons), or alkoxy (1-4 carbons), or any combination thereof; andX.sub.fl is covalently linked to the oxygen --O-- at C.sub.1.

    Abstract translation: 通常包括喹唑啉酮（喹唑啉酮），苯并咪唑，苯并噻唑，苯并恶唑，喹啉，吲哚啉和菲啶的一类荧光团制备的新型荧光沉淀基质，其具有-C1 = C2-的碳原子的通式为： 以便完成可含有至少一个杂原子N，O或S的第一个5-或6-元芳环，其中-C4-N = C3-的碳原子进一步连接，以便完成一个 第二个5-或6-元芳环，其至少包含C3和C4之间的氮，并且可以含有至少一个另外的杂原子N，O或S，其中第一和第二芳环可以通过5-或6 至少含有来自第一芳环的C2和来自第二芳环的C3，所述桥连环可以是饱和或不饱和的，并且可以含有杂原子N，O或S，其中第一个 并且第二芳环可以稠合 o至少一个可以含有至少一个杂原子N，O或S的另外的芳环，并且其中每个所述芳环可以通过用芳基碳上的任何氢取代而被进一步修饰，所述取代基是卤素，硝基 ，氰基，芳基，低级烷基（1-4个碳），全氟烷基（1-4个碳）或烷氧基（1-4个碳），或其任何组合; 并且Xfl在C1处与氧-O-共价连接。

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