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    Detection of immobilized nucleic acid
    8.
    发明申请
    Detection of immobilized nucleic acid 有权
    检测固定化核酸

    公开(公告)号：US20050239096A1

    公开(公告)日：2005-10-27

    申请号：US10956868

    申请日：2004-09-30

    申请人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

    发明人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

    IPC分类号： C07D413/02 ,  C07D417/02 ,  C07D417/06 ,  C09B23/02 ,  C12Q1/68 ,  C07D43/02

    CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

    摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

    Methine dyes, manufacturing methods thereof, and silver halide photographic materials containing same dyes
    10.
    发明授权
    Methine dyes, manufacturing methods thereof, and silver halide photographic materials containing same dyes 有权
    甲基染料，其制造方法和含有相同染料的卤化银照相材料

    公开(公告)号：US06884891B2

    公开(公告)日：2005-04-26

    申请号：US10192590

    申请日：2002-07-11

    申请人： Katsumi Kobayashi

    发明人： Katsumi Kobayashi

    IPC分类号： C07D263/56 ,  C07D277/64 ,  C07D417/14 ,  C09B23/00 ,  C09B23/01 ,  C09B23/02 ,  C09B23/10 ,  G03C1/12

    CPC分类号： C07D263/56 ,  C07D277/64 ,  C07D417/14 ,  C09B23/0066 ,  C09B23/0083 ,  C09B23/02 ,  C09B23/10 ,  G03C1/12

    摘要： Methine dye compounds represented by formula (1) and highly sensitive silver halide photographic materials containing these compounds: Dye1-(L1-(Dye2)m1)m2 (CI1)y1  (1) wherein L1 represents a linkage group represented by formula (2), m1 represents an integer of 1 to 5, m2 represents an integer of 1 to 5, Dye1 represents a first chromophore, Dye 2 represents a second chromophore, CI1 represents an ion for neutralization of electric charge, and y1 represents a number of the ions required for neutralization of electric charges; -G1-A1-Y1-G2-Y2-A2-G3-  (2) wherein A1 and A2 each individually represent a carbonyl group or a sulfonyl group, Y1 and Y2 each individually represent —O—, —S— or —NR1—, R1 represents a hydrogen atom, an alkyl group, an alkenyl group, an aryl group or a heterocyclic group, and G1, G2 and G3 each individually represent a divalent linkage group.

    摘要翻译： 由式（1）表示的甲基染料化合物和含有这些化合物的高灵敏度卤化银照相材料：<？in-line-formula description =“In-line Formulas”end =“lead”→Dye1-（L 1 （1）（1）＆lt;＆lt; 1＆gt;（1）＆lt; -line-formula description =“In-line Formulas”end =“tail”？>其中L 1表示由式（2）表示的连接基团，m1表示1〜5的整数，m 2 表示1〜5的整数，染料1表示第一发色团，染料2表示第二发色团，CI 1表示用于中和电荷的离子，y1表示中和所需的离子数 的电费; <？in-line-formula description =“In-line Formulas”end =“lead”？> - G <1> 1 - （2）<α-2 2 line-formula description =“In-line Formulas”end =“tail”？>其中A 1和A 2各自各自表示羰基或磺酰基，Y 1个和Y 2个各自各自独立地表示-O - ， - S-或-NR 1 - ， - R 1， 表示氢原子，烷基，烯基，芳基或杂环基，G 1，G 2和G 3 >各自独立地表示二价连接基团。