公开(公告)号：US20120244608A1

公开(公告)日：2012-09-27

申请号：US13134157

申请日：2011-05-31

Applicant: Mark Selker ,  Barbara Paldus ,  Timothy Johnston

Inventor： Mark Selker ,  Barbara Paldus ,  Timothy Johnston

IPC: C12M1/34 ,  B32B38/16 ,  C12M1/00

CPC classification number: C12M41/00 ,  C12M23/14 ,  C12M23/28 ,  C12M27/16 ,  G01N21/359 ,  G01N21/552 ,  G01N21/65 ,  G01N21/783 ,  G01N21/80 ,  G01N2021/7786

Abstract: A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

Abstract translation: 一种用于监测适用于聚合物生物过程容器或与下游设备的生物过程的复合传感器组件，包括：i）包含具有中空管状部分和底板部分的高表面张力热塑性塑料的端口， 板在其壁的孔处易于密封到生物反应器容器; ii）包括电和/或光学测量部件的通常不透明的聚合物监测传感器组件。 传感器组件装配在端口的中空管状部分的孔内，并且粘合地保持在其中。

公开(公告)号：US20110060463A1

公开(公告)日：2011-03-10

申请号：US12151254

申请日：2008-05-05

Applicant: Mark Selker ,  Barbara Paldus

Inventor： Mark Selker ,  Barbara Paldus

IPC: G06F19/00

CPC classification number: G05B23/0221 ,  C12M41/48

Abstract: A bioreactor monitoring and/or control system utilizing only non-dedicated user input and information display devices, a digital controller and software, said system comprising:i) at least one diagnostic probe for measuring operating conditions in a bioreactor; ii) signal conditioning and communication electronics which supply operating current and/or voltage to said probe and which convert the diagnostic signal into a format accessible by iii) a digital controller which directly receives the format converted signal and transmits it to iv) software which enables the display device to show the converted signal, and also to instruct the controller to implement changes in the operating conditions in the reactor, and v) a software-based virtual transmitter which substantially replicates the keyboard, display, menu-tree and response of a physical sensor transmitter.

Abstract translation: 一种仅使用非专用用户输入和信息显示装置的生物反应器监测和/或控制系统，数字控制器和软件，所述系统包括：i）至少一个诊断探针，用于测量生物反应器中的操作条件; ii）信号调节和通信电子设备，其向所述探针提供工作电流和/或电压，并且将诊断信号转换成可由以下格式访问的格式：iii）数字控制器，其直接接收格式转换的信号并将其发送到iv）使能 所述显示装置显示转换的信号，并且还指示控制器实现反应堆中的操作条件的变化，以及v）基本上复制键盘，显示，菜单树和响应的基于软件的虚拟发射器 物理传感器发射器。

公开(公告)号：US20080274541A1

公开(公告)日：2008-11-06

申请号：US12008447

申请日：2008-01-10

Applicant: Mark Selker ,  Barbara Paldus

Inventor： Mark Selker ,  Barbara Paldus

IPC: C12M1/00

CPC classification number: B01F11/0025 ,  B01F3/04248 ,  B01F11/0014 ,  B01F11/0017 ,  B01F15/00831 ,  B01F15/0085 ,  B01F15/00876 ,  B01F15/00883 ,  C12M23/02 ,  C12M23/28 ,  C12M27/10 ,  C12M27/16 ,  C12M27/20

Abstract: A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.

Abstract translation: 公开了一种圆柱形或环形聚合物生物反应器，其提供增强的生长培养基的混合和通气，同时提供降低的机械剪切力。

公开(公告)号：US20080170224A1

公开(公告)日：2008-07-17

申请号：US11724818

申请日：2007-03-16

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

IPC: G01J3/30

CPC classification number: G01N21/6428 ,  G01N2021/6441

Abstract: Apparatus and method for the stimulation and collection of fluorescent signals, said apparatus comprising: i) a frequency modulated optical source which emits excitation light of a wavelength which will stimulate a target fluorophore to emit a fluorescent signal when illuminated by said excitation light: ii) a first optical filter interposed between said optical source and said target fluorophore, iii) a second optical filter interposed between said fluorophore and a photo-detector positioned to receive said emitted fluorescent signal, and iv) a data processor which calculates and records the phase delay between said excitation light and said fluorescent signal wherein the beam path of at least one of the excitation light and the fluorescent signal is transmitted substantially through free space.

Abstract translation: 用于刺激和收集荧光信号的装置和方法，所述装置包括：i）调频光源，其发射波长的激发光，其将在被所述激发光照射时刺激目标荧光团发射荧光信号：ii） 插入在所述光源和所述目标荧光团之间的第一滤光器，iii）置于所述荧光团和位于接收所述发射的荧光信号的光检测器之间的第二滤光器，以及iv）数据处理器，其计算并记录相位延迟 在所述激发光和所述荧光信号之间，其中激发光和荧光信号中的至少一个的光束路径基本上透过自由空间传播。

公开(公告)号：US20080114550A1

公开(公告)日：2008-05-15

申请号：US11702861

申请日：2007-02-06

Applicant: Barbara Paldus ,  Mark Selker

Inventor： Barbara Paldus ,  Mark Selker

IPC: G01N33/48

CPC classification number: C12M41/36

Abstract: A method for converting non-linear optical loss readings in a bioreactor process vessel into process parameter units by applying a curve fitting algorithm to the fitting function represented by the empirical equation: yol = A + B ( 1 -  - x PU C ) + D · x PU wherein xPU is in the process units (PU), yol is optical loss in the chosen units, A is the offset, D is the absorption coefficient, B is the effective scattering coefficient, and C is the scattering constant. The preferred fitting algorithm is Levenberg-Marquardt.

Abstract translation: 通过对由经验公式表示的拟合函数应用曲线拟合算法，将生物反应器处理容器中的非线性光学损耗读数转换为过程参数单元的方法：