公开(公告)号：US07943753B2

公开(公告)日：2011-05-17

申请号：US12620840

申请日：2009-11-18

申请人： Emil M. Orozco, Jr. ,  Rebecca E. Cahoon ,  Wesley B. Bruce ,  Yong Tao ,  Zude Weng

发明人： Emil M. Orozco, Jr. ,  Rebecca E. Cahoon ,  Wesley B. Bruce ,  Yong Tao ,  Zude Weng

IPC分类号： C12N15/29

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US20100077505A1

公开(公告)日：2010-03-25

申请号：US12620840

申请日：2009-11-18

申请人： Emil M. Orozco, JR. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

发明人： Emil M. Orozco, JR. ,  Zude Weng ,  Wesley B. Bruce ,  Rebecca E. Cahoon ,  Yong Tao

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/10

CPC分类号： C07K14/415 ,  C12N15/8294

摘要： This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

摘要翻译： 本发明涉及编码生长素转运蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或大部分生长素转运蛋白的嵌合基因，其中嵌合基因的表达导致转化宿主中生长素转运蛋白水平的变化 细胞。 本发明还涉及使用生长素转运蛋白调节根发育的方法，以及发现具有潜在除草活性的化合物。

公开(公告)号：US06617498B1

公开(公告)日：2003-09-09

申请号：US09655024

申请日：2000-09-05

申请人： Wesley B. Bruce ,  Carl W. Garnaat ,  Steven W. Ritchie

发明人： Wesley B. Bruce ,  Carl W. Garnaat ,  Steven W. Ritchie

IPC分类号： A01H500

CPC分类号： C12N15/8237 ,  C12N15/8238 ,  C12N15/8279

摘要： The present invention provides compositions and methods for regulating the expression of nucleotide sequence of interest in a plant. Compositions include a novel nucleotide sequence comprising an inducible promoter for the 5C9 gene, which encodes a maize patatin-like polypeptide. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the inducible promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in an inducible manner.

摘要翻译： 本发明提供用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物包括一个新的核苷酸序列，其包含5C9基因的诱导型启动子，其编码玉米patatin样多肽。 提供了使用本文公开的启动子序列在植物中表达目的核苷酸序列的方法。 该方法包括将可操作地连接到本发明的诱导型启动子的核苷酸序列稳定地并入植物细胞的基因组中，并以可诱导的方式再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US06518483B1

公开(公告)日：2003-02-11

申请号：US09444905

申请日：1999-11-22

申请人： Wesley B. Bruce ,  Lynne E. Sims

发明人： Wesley B. Bruce ,  Lynne E. Sims

IPC分类号： A01H500

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8227

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码Knox1，Kn1样同源框基因和从其分离的序列的基因的根优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US6072050A

公开(公告)日：2000-06-06

申请号：US28819

申请日：1998-02-24

申请人： Benjamin A. Bowen ,  Wesley B. Bruce ,  Guihua Lu ,  Lynne E. Sims ,  Laura A. Tagliani

发明人： Benjamin A. Bowen ,  Wesley B. Bruce ,  Guihua Lu ,  Lynne E. Sims ,  Laura A. Tagliani

IPC分类号： C12N9/02 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H4/00 ,  C12N15/00

CPC分类号： C12N9/0008 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8279

摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.

公开(公告)号：US5196329A

公开(公告)日：1993-03-23

申请号：US587517

申请日：1990-09-24

申请人： William B. Gurley ,  Wesley B. Bruce

发明人： William B. Gurley ,  Wesley B. Bruce

IPC分类号： C12N15/67 ,  C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/67

摘要： A DNA sequence element, which activates or enhances expression of genes in plants has been identified and characterized. In particular, DNA segments isolated from the upstream region of the T-DNA 780 gene are capable of activating or increasing transcription levels of plant-expressible genes in recombinant DNA-containing plant tissue. The 780 gene transcription activator is useful in general for increasing the level of expression of a gene in a plant. In particular, such activators are useful in the construction of plant-expression complexes which contain a plant-expressible gene placed under the regulatory control of the activator. Such expression complexes can be introduced into plant tissue where the inserted gene is expressed.

摘要翻译： 已经鉴定并表征了一种激活或增强植物中基因表达的DNA序列元件。 特别地，从T-DNA 780基因的上游区域分离的DNA片段能够激活或增加重组含DNA植物组织中植物可表达基因的转录水平。 780基因转录激活因子通常用于增加植物中基因表达水平。 特别地，这种激活剂可用于构建植物表达复合物，其中含有置于活化剂调控下的植物可表达基因。 可以将这样的表达复合物引入植入组织中，其中插入的基因被表达。