公开(公告)号：US20080021206A1

公开(公告)日：2008-01-24

申请号：US10591291

申请日：2005-03-02

申请人： Mitsuo Sekine ,  Kohji Seio ,  Hisao Saneyoshi

发明人： Mitsuo Sekine ,  Kohji Seio ,  Hisao Saneyoshi

IPC分类号： C07H21/02 ,  C07H19/06

CPC分类号： C07H19/067 ,  C07H19/167 ,  Y02P20/55

摘要： The purpose of the present invention is to develop a system for efficiently constituting cyanoethyl ethers under mild conditions and probability of the ethers as functional groups for imparting specific functions and to make a contribution to the production of novel functional nucleic acids. The present invention relates to a nucleoside that is represented by the general formula (I) or a nucleotide derived therefrom: wherein X and Y may be the same as or different from each other, and are hydrogen, optionally substituted silyl group, 4-methoxytrityl group, 4,4′-dimethoxytrityl group or a group represented by the general formula (II): wherein R1 and R2 may be the same as or different from each other, representing an alkyl group having 1-7 carbon atoms such as diisopropyl, or they are united with each other to form a ring structure, R3 represents a protective group for a phosphoric acid such as 2-cyanoethyl; and B1 represents an optionally substituted pyrimidine or purine base.

摘要翻译： 本发明的目的是开发一种在温和条件下有效构成氰乙基醚的体系，以及作为赋予特定功能的醚作为官能团并有助于新型功能性核酸生产的可能性。 本发明涉及由通式（I）表示的核苷或由其衍生的核苷酸：其中X和Y可以相同或不同，为氢，任选取代的甲硅烷基，4-甲氧基三苯甲基 基团，4,4'-二甲氧基三苯甲基或由通式（II）表示的基团：其中R 1和R 2可以彼此相同或不同，表示具有1-7个碳原子的烷基，例如二异丙基， 或者它们相互结合形成环结构，R 3表示磷酸如2-氰基乙基的保护基; 而B1代表任选取代的嘧啶或嘌呤碱。

公开(公告)号：US20070172826A1

公开(公告)日：2007-07-26

申请号：US10590232

申请日：2005-02-23

申请人： Mitsuo Sekine ,  Kohji Seio ,  Masahiro Mizuta ,  Takeshi Terada

发明人： Mitsuo Sekine ,  Kohji Seio ,  Masahiro Mizuta ,  Takeshi Terada

IPC分类号： C12Q1/68 ,  C07F17/02

CPC分类号： C07D403/14 ,  C07F17/02 ,  C12Q1/6818

摘要： The purpose of the present invention is to provide a compound that specifically binds to the base sequence of a double-stranded nucleic acid molecule. The compound can reduce the electrochemical signal/noise ratio (S/N) in electrochemical detection, and as a result, the detection sensitivity (precision) will be greatly improved so as to enable the determination of an ultratrace amount of nucleic acid molecule. The present invention relates to a ferrocene compound represented by the following general formula (I): wherein “A” represents a divalent ferrocene-containing linker or ferrocene-1,1′-yl, R2 represents a hydrogen atom or alkyl; “n” and “m” represent any natural numbers; and “V” and “X” represent the pyrrole-imidazole-polyamide (PIPA); to a ligand consisting of said ferrocene compound for sequence-specific detection of double-stranded nucleic acid molecules; to a method for electrochemical detection of double-stranded nucleic acid molecules 8 with the use of said ligand; and to an apparatus or device for the electrochemical detection with the use of said ligand.

摘要翻译： 本发明的目的是提供特异性结合双链核酸分子的碱基序列的化合物。 该化合物可以降低电化学检测中的电化学信噪比（S / N），结果检测灵敏度（精度）将大大提高，从而能够测定超临界量的核酸分子。 本发明涉及由以下通式（I）表示的二茂铁化合物：其中“A”表示含二茂铁的连接体或二茂铁-1,1'-基，R 2表示 氢原子或烷基; “n”和“m”表示任何自然数; “V”和“X”表示吡咯 - 咪唑 - 聚酰胺（PIPA）。 涉及由所述二茂铁化合物组成的配体，用于双链核酸分子的序列特异性检测; 涉及使用所述配体进行电泳检测双链核酸分子8的方法; 以及使用所述配体进行电化学检测的装置或装置。

公开(公告)号：US20070133150A1

公开(公告)日：2007-06-14

申请号：US11561020

申请日：2006-11-17

申请人： Mitsuo Sekine ,  Yutaka Noguchi ,  Makoto Seo

发明人： Mitsuo Sekine ,  Yutaka Noguchi ,  Makoto Seo

IPC分类号： H02B1/00

CPC分类号： H01R9/2608 ,  H05K7/1465

摘要： A signal input/output device includes an array of signal input/output units. The signal input/output units have respective bases, each of which includes a connecting member. The connecting member has a second protruding end finger swingably supported on a base, and a first protruding end finger that engages with a stop of an adjacent base. The connecting member is positioned and fixed (secured) by a screw, which extends through a bottom plate and is threaded in a square nut. The connecting member, when secured in this manner, joins the adjacent bases to each other.

摘要翻译： 信号输入/输出装置包括信号输入/输出单元阵列。 信号输入/输出单元具有各自的基座，每个基座包括连接构件。 连接构件具有可摆动地支撑在基座上的第二突出端指，以及与相邻基座的止动件接合的第一突出端指。 连接构件通过螺钉定位和固定（固定），该螺钉延伸穿过底板并且拧入方形螺母。 当以这种方式固定时，连接构件将相邻的基座相互连接。

公开(公告)号：US4255803A

公开(公告)日：1981-03-10

申请号：US921508

申请日：1978-07-03

申请人： Mitsuo Sekine

发明人： Mitsuo Sekine

IPC分类号： G04G13/02 ,  G04B23/02

CPC分类号： G04G13/023

摘要： In an electronic timepiece equipped with an alarm function, an alarm time memory circuit is provided for setting in an alarm time by actuation of an external control member and another memory circuit is provided into which an advance warning time period can be set and stored. Means are provided for detecting coincidence between the current time and an advance warning time comprising the difference between the memorized alarm time and the advance warning time period, to provide an advance warning signal prior to the actual alarm time. Parallel computation is used to generate said advance warning time, and the advance warning time period can be continuously displayed.

摘要翻译： 在具有报警功能的电子钟表中，提供了一个报警时间存储电路，用于通过外部控制部件的动作来设定报警时间，并且提供另一个存储电路，可设定和存储提前警告时间段。 提供了用于检测当前时间与包括存储的闹钟时间和提前警告时间段之间的差的提前警告时间的装置，以在实际报警时间之前提供提前警告信号。 并行计算用于产生所述提前警告时间，并且可以连续地显示提前警告时间段。