公开(公告)号：US5773697A

公开(公告)日：1998-06-30

申请号：US636283

申请日：1996-04-23

申请人： Dwight T. Tomes ,  Bin Huang ,  Paul D. Miller

发明人： Dwight T. Tomes ,  Bin Huang ,  Paul D. Miller

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  A01H5/00

CPC分类号： C12N15/8294 ,  C12N15/8287

摘要： The invention discloses a transgenic method for producing fruit with diminished or very little seeds or fruits with reduced seed number. It involves the temporal expression of a cytotoxic gene or combination of genes targeted toward arresting seed development at a time sufficiently after pollination that fruit development and maturation is normal while early enough in seed development that seed maturation per se is minimized. The invention also includes transgenic constructs, vectors, and methods for production of the fruit with diminished or very little seed bearing plants.

摘要翻译： 本发明公开了一种转基因方法，用于生产具有减少的种子数目减少或极少的种子或果实的水果。 它涉及细胞毒性基因的时间表达或在授粉后一段时间足以阻止种子发育的基因的组合的时间表达，即果实发育和成熟是正常的，而种子发育中的早期种子成熟本身最小化。 本发明还包括转基因构建体，载体和用于生产具有减少或极少种子植物的水果的方法。

公开(公告)号：US08026411B2

公开(公告)日：2011-09-27

申请号：US12622891

申请日：2009-11-20

申请人： Dwight T. Tomes ,  Christopher Zinselmeier ,  Jeffrey E. Habben

发明人： Dwight T. Tomes ,  Christopher Zinselmeier ,  Jeffrey E. Habben

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  C12N15/8245 ,  Y02A40/146

摘要： The invention discloses a transgenic method for enhancing sink strength in female reproductive organs. It involves the temporal and spatial expression of genes affecting the starch and sucrose metabolic pathways in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase yield potential over a range of environmental conditions.

摘要翻译： 本发明公开了一种提高女性生殖器官的吸收强度的转基因方法。 它涉及影响植物器官或植物组织中淀粉和蔗糖代谢途径的基因的时间和空间表达。 本发明还包括用于生产具有可遗传表型的作物植物的表达构建体和方法，其可用于在一系列环境条件下增加产量潜力的育种程序中。

公开(公告)号：US20080131896A1

公开(公告)日：2008-06-05

申请号：US11941106

申请日：2007-11-16

申请人： Jerome P. Ranch ,  Wallace A. Marsh ,  Terry EuClaire Meyer ,  Dwight T. Tomes ,  Zuo-Yu Zhao ,  Gan-Yuan Zhong ,  Jimei Wang ,  Jeffery A. Mullen ,  Christopher J. Scelonge ,  Glenn S. Cole ,  John F. Soper

发明人： Jerome P. Ranch ,  Wallace A. Marsh ,  Terry EuClaire Meyer ,  Dwight T. Tomes ,  Zuo-Yu Zhao ,  Gan-Yuan Zhong ,  Jimei Wang ,  Jeffery A. Mullen ,  Christopher J. Scelonge ,  Glenn S. Cole ,  John F. Soper

IPC分类号： C12Q1/68

CPC分类号： C12N15/8202 ,  A01H1/04 ,  A01H5/10 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8207 ,  C12N15/8209 ,  C12Q1/6855 ,  C12Q1/6895 ,  C12Q2600/156

摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.

摘要翻译： 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。

公开(公告)号：US07148401B2

公开(公告)日：2006-12-12

申请号：US10931077

申请日：2004-08-31

申请人： Dilbag Multani ,  Xiaomu Niu ,  Dwight T. Tomes ,  Haiyin Wang ,  Deping Xu

发明人： Dilbag Multani ,  Xiaomu Niu ,  Dwight T. Tomes ,  Haiyin Wang ,  Deping Xu

IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8223

摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.

摘要翻译： 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。