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1.发明申请公开(公告)号：US20100071087A1
公开(公告)日：2010-03-18
申请号：US12622891
申请日：2009-11-20
CPC分类号： C12N15/8261 , C12N15/8245 , Y02A40/146
摘要： The invention discloses a transgenic method for enhancing sink strength in female reproductive organs. It involves the temporal and spatial expression of genes affecting the starch and sucrose metabolic pathways in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase yield potential over a range of environmental conditions.
摘要翻译： 本发明公开了一种提高女性生殖器官的吸收强度的转基因方法。 它涉及影响植物器官或植物组织中淀粉和蔗糖代谢途径的基因的时间和空间表达。 本发明还包括用于生产具有可遗传表型的作物植物的表达构建体和方法，其可用于在一系列环境条件下增加产量潜力的育种程序中。
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2.发明授权Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize D9 gene and methods of use 失效对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子及其使用方法公开(公告)号：US07557266B2
公开(公告)日：2009-07-07
申请号：US11736615
申请日：2007-04-18
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译： 本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。 本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。
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公开(公告)号：US07544857B2
公开(公告)日：2009-06-09
申请号：US11589656
申请日：2006-10-30
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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公开(公告)号：US07098380B2
公开(公告)日：2006-08-29
申请号：US10260046
申请日：2002-09-27
CPC分类号： C12N9/1059 , C12N15/8246
摘要： The present invention provides compositions and methods for manipulation of plant polysaccharides and plant polysaccharide synthases. Compositions include novel nucleotide sequences encoding polysaccharide synthases polypeptides, and biologically active variants thereof. Further provided are methods for polysaccharide manipulation using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. Methods for enhancing digestibility of plants, improving gum extractability from plants, and altering plant growth are also provided.
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公开(公告)号：US06921815B2
公开(公告)日：2005-07-26
申请号：US10109488
申请日：2002-03-28
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
IPC分类号： C07K14/415 , C12N9/06 , C12N15/82 , C12N15/00 , C12N15/09
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8223 , C12N15/8295
摘要： The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。 该组合物是启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US06570067B1
公开(公告)日：2003-05-27
申请号：US09363891
申请日：1999-07-30
IPC分类号： C12N1582
CPC分类号： C12N15/8207
摘要： The invention provides a method for producing stably transformed dicotyledonous plants, via the introduction of a foreign DNA, containing an expression vector carrying a gene of interest, into dicotyledonous cells via one or more microparticle bombardments to produce transformed dicotyledonous cells; followed by the regeneration of a stably transformed fertile dicotyledonous plant from the transformed dicotyledonous cells.
摘要翻译： 本发明提供一种通过一种或多种微粒轰击通过将含有携带目的基因的表达载体的外来DNA引入双子叶植物培养物来产生稳定转化的双子叶植物以产生转化的双子叶植物的方法。 随后从转化的双子叶植物细胞中再生稳定转化的可育双子叶植物。
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7.发明授权Transgenic methods and compositions for producing parthenocarpic fruits and vegetables 失效用于生产单性果实水果和蔬菜的转基因方法和组合物
公开(公告)号：US5877400A
公开(公告)日：1999-03-02
申请号：US641479
申请日：1996-05-01
CPC分类号： C12N15/8249 , C12N15/8287 , C12N15/8297
摘要： The invention discloses a transgenic method for producing parthenocarpic fruits or fruits with reduced seed number. It involves the temporal expression of a plant hormone or precursor or other such gene so that gibberellin or other similar hormonal activity involved in initiating fruit set activity is potentiated. The gene is operably linked to a regulatory promoter so that expression is timed prior to pollen development or fertilization. Expression of the hormone causes fruit development in the absence of fertilization. The method also results in a fruit that has diminished or very little seed. The invention also includes transgenic constructs, vectors, and methods for production of the parthenocarpic plants.
摘要翻译： 本发明公开了一种用于生产具有降低种子数的单性果实或果实的转基因方法。 它涉及植物激素或前体或其他此类基因的时间表达，使得涉及起始果实活性的赤霉素或其它相似的激素活性被增强。 该基因与调节性启动子可操作地连接，使得在花粉发育或受精前表达是定时的。 激素的表达在没有受精的情况下导致果实发育。 这种方法也会导致果实的种子减少或很少。 本发明还包括用于生产单性结实植物的转基因构建体，载体和方法。
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公开(公告)号：US20100325752A1
公开(公告)日：2010-12-23
申请号：US12819888
申请日：2010-06-21
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物和方法包括表达盒和转化植物，并提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US07612254B2
公开(公告)日：2009-11-03
申请号：US11494950
申请日：2006-07-28
CPC分类号： C12N9/1059 , C12N15/8246
摘要： The present invention provides compositions and methods for manipulation of plant polysaccharides and plant polysaccharide synthases. Compositions include novel nucleotide sequences encoding polysaccharide synthases polypeptides, and biologically active variants thereof. Further provided are methods for polysaccharide manipulation using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译： 本发明提供了用于操作植物多糖和植物多糖合酶的组合物和方法。 组合物包括编码多糖合酶多肽的新型核苷酸序列及其生物活性变体。 进一步提供使用本文公开的序列的多糖操作的方法。 一种方法包括稳定地并入植物细胞的基因组中，本发明的核苷酸序列可操作地连接到异源启动子并再生表达核苷酸序列的稳定转化的植物。
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公开(公告)号：US20080172760A1
公开(公告)日：2008-07-17
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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