公开(公告)号：US06017434A

公开(公告)日：2000-01-25

申请号：US438231

申请日：1995-05-09

申请人： John W. Simpson ,  Jonathan Marc Rothberg ,  Gregory T. Went

发明人： John W. Simpson ,  Jonathan Marc Rothberg ,  Gregory T. Went

IPC分类号： B01J19/00 ,  G01N27/447 ,  G01N27/26

CPC分类号： B01J19/0093 ,  G01N27/44708 ,  G01N27/44782 ,  G01N27/44791 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00873 ,  B01J2219/00995 ,  G01N27/44721 ,  Y10S436/80 ,  Y10T436/143333

摘要： This invention is an integrated instrument for the high-capacity electrophoretic analysis of biopolymer samples. It comprises a specialized high-voltage, electrophoretic module in which the migration lanes are formed between a bottom plate and a plurality of etched grooves in a top plate, the module permitting concurrent separation of 80 or more separate samples. In thermal contact with the bottom plate is a thermal control module incorporating a plurality of Peltier heat transfer devices for the control of temperature and gradients in the electrophoretic medium. Fragments are detected by a transmission imaging spectrograph which simultaneously spatially focuses and spectrally resolves the detection region of all the migration lanes. The spectrograph comprises a transmission dispersion element and a CCD array to detect signals. Signal analysis comprises the steps of noise filtering, comparison in a configuration space with signal prototypes, and selection of the best prototype. Optionally post-processing is done by a Monte-Carlo simulated annealing algorithm to improve results. Optionally, an array of micro-reactors can be integrated into the instrument for the generation of sequencing reaction fragments directly from crude DNA samples.

公开(公告)号：US5698977A

公开(公告)日：1997-12-16

申请号：US490441

申请日：1995-06-05

申请人： John W. Simpson ,  James P. Fulton ,  Russell A. Wincheski ,  Ronald G. Todhunter ,  Min Namkung ,  Shridhar C. Nath

发明人： John W. Simpson ,  James P. Fulton ,  Russell A. Wincheski ,  Ronald G. Todhunter ,  Min Namkung ,  Shridhar C. Nath

IPC分类号： G01N27/90 ,  G01N3/32 ,  G01N19/08 ,  G01R33/12

CPC分类号： G01N27/9033 ,  G01N27/902

摘要： Flux-focusing electromagnetic sensor using a ferromagnetic flux-focusing lens simplifies inspections and increases detectability of fatigue cracks and material loss in high conductivity material. A ferrous shield isolates a high-turn pick-up coil from an excitation coil. Use of the magnetic shield produces a null voltage output across the receiving coil in presence of an unflawed sample. Redistribution of the current flow in the sample caused by the presence of flaws, eliminates the shielding condition and a large output voltage is produced, yielding a clear unambiguous flaw signal. Maximum sensor output is obtained when positioned symmetrically above the crack. By obtaining position of maximum sensor output, it is possible to track the fault and locate the area surrounding its tip. Accuracy of tip location is enhanced by two unique features of the sensor; a very high signal-to-noise ratio of the probe's output resulting in an extremely smooth signal peak across the fault, and a rapidly decaying sensor output outside a small area surrounding the crack tip enabling the search region to be clearly defined. Under low frequency operation, material thinning due to corrosion causes incomplete shielding of the pick-up coil. Low frequency output voltage of the probe is therefore a direct indicator of thickness of the test sample. Fatigue testing a conductive material is accomplished by applying load to the material, applying current to the sensor, scanning the material with the sensor, monitoring the sensor output signal, adjusting material load based on the sensor output signal of the sensor, and adjusting position of the sensor based on its output signal.

摘要翻译： 使用铁磁通量聚焦透镜的磁通聚焦电磁传感器简化了检查，增加了高导电性材料中疲劳裂纹和材料损失的可检测性。 铁屏蔽将高转弯拾取线圈与励磁线圈隔离。 使用磁屏蔽在存在未裂纹的样品的情况下，在接收线圈上产生零电压输出。 由于存在缺陷导致样品中的电流重新分布，消除了屏蔽状况，产生了大的输出电压，产生了明确的明确的缺陷信号。 当对称地位于裂纹上方时获得最大传感器输出。 通过获得最大传感器输出的位置，可以跟踪故障并定位其尖端周围的区域。 传感器的两个独特功能增强了尖端位置的精度; 探头输出的非常高的信噪比导致故障之间的非常平滑的信号峰值，以及在裂纹尖端周围的小区域外的迅速衰减的传感器输出，使得能够清楚地定义搜索区域。 在低频操作下，由于腐蚀引起的材料变薄会引起拾音线圈的不完全屏蔽。 因此，探头的低频输出电压是测试样品厚度的直接指示。 通过对材料施加负载，向传感器施加电流，用传感器扫描材料，监测传感器输出信号，基于传感器的传感器输出信号调整材料载荷，以及调整位置 该传感器基于其输出信号。